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|標題:||Low density supercritical fluids precipitation of 9-cis and all trans-β-carotenes enriched particulates from Dunaliella salina||關鍵字:||9-cis-β-Carotene, trans-β-carotene;Supercritical anti-solvent Pulverization;Elution chromatography;Microalgae||Project:||Journal of Chromatography A, Volume 1299, Page(s) 1-9.||摘要:||
In this study, supercritical anti-solvent (SAS) pulverization coupled with reverse phase elution chromatography was employed to isolate 9-cis and trans-β-carotenes from Dunaliella salina. Total concentration of 9-cis (134.7 mg/g) and trans-β-carotene (204.2 mg/g) was increased from 338.9 mg/g of the ultrasonic extract to 859.7 mg/g (338.9 for 9-cis and 520.8 for trans) of the elution fraction. The SAS pulverization of the collected fraction further produced submicron-sized particulates containing 932.1 mg/g (355.6 for 9-cis and 576.5 for trans) of total β-carotenes with a recovery of 86.3% (83.9% for cis and 87.8% for trans). Effects of two SAS operational conditions on the purity, recovery of total β-carotenes, mean size and morphology of the precipitates were obtained from an experimentally designed method. Generation of micronized particulates enriched with 9-cis and trans-β-carotenes by low-density SAS was proved to be feasible and environmental benign.
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