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標題: A label-free impedimetric DNA sensing chip integrated with AC electroosmotic stirring
關鍵字: Label-free;Electrochemical impedance spectroscopy;AC electroosmosis;DNA sensor
Project: Biosensors and Bioelectronics, Volume 43, Page(s) 348-354.
AC electroosmosis (ACEO) flow and label-free electrochemical impedance spectroscopy are employed to increase the hybridization rate and specifically detect target DNA (tDNA) concentrations. A low-ionic-strength solution, 6.1 μS/cm 1 mM Tris (pH 9.3), was used to produce ACEO and proved the feasibility of hybridization. Adequate voltage parameters for the simultaneous ACEO driving and DNA hybridization in the 1 mM Tris solution were 1.5 Vpp and 200 Hz. Moreover, an electrode set with a 1:4 ring width-to-disk diameter ratio exhibited a larger ACEO velocity above the disk electrode surface to improve collecting efficiency. The ACEO-integrated DNA sensing chips could reach 90% saturation hybridization within 117 s. The linear range and detection limit of the sensors was 10 aM–10 pM and 10 aM, respectively. The label-free impedimetric DNA sensing chips with integrated ACEO stirring can perform rapid hybridization and highly-sensitive detections to specifically measure tDNA concentrations.
DOI: 10.1016/j.bios.2012.12.041
Appears in Collections:生物產業機電工程學系

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