Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/85207
DC FieldValueLanguage
dc.creatorYing-Hong Linen_US
dc.creatorChing-Chung Suen_US
dc.creatorChih-Ping Chaoen_US
dc.creatorChi-Yu Chenen_US
dc.creatorChung-Jan Changen_US
dc.creatorJenn-Wen Huangen_US
dc.creatorPi-Fang Linda Changen_US
dc.date2013-02zh_TW
dc.date.accessioned2014-12-03T06:12:18Z-
dc.date.available2014-12-03T06:12:18Z-
dc.identifier.urihttp://hdl.handle.net/11455/85207-
dc.description.abstractThe Fusarium genus causes devastatingplant diseases worldwide, in which Fusarium oxysporumis the most serious crop pathogen. Disease monitoringis the basis of integrated pest management ofany disease. The lack of rapid, accurate, and reliabledevice to detect and identify plant pathogens is one ofthe main limitations in integrated disease management.This study describes an efficient and quantifiable diagnosismethod for the specific detection of F. oxysporumf. sp. cubense (Foc) race 4 in field-infected banana.With the optimized PCR parameters using the SCAR(sequence characterized amplified region) primersFocSc-1/FocSc-2 and a real-time PCR strategy, thedeveloped method showed high reproducibility andwas very sensitive to detect extremely low quantitiesof Foc genomic DNA (gDNA).We also found that FocgDNA in severely symptomatic banana pseudostemsand leaves were 6946-fold and 26.69-fold more than inthose of mild-symptomatic banana, respectively.en_US
dc.format.medium期刊論文zh_TW
dc.language.isoen_USzh_TW
dc.relationEuropean Journal of Plant Pathology, Volume 135, Issue 2, Page(s) 395-405.en_US
dc.relation.urihttp://link.springer.com/article/10.1007%2Fs10658-012-0096-0-
dc.subjectFusarium oxysporum fen_US
dc.subjectspen_US
dc.subjectcubenseen_US
dc.subjectFusarium wilten_US
dc.subjectMolecular detectionen_US
dc.subjectReliablediagnosisen_US
dc.subjectReal-time PCRen_US
dc.subjectSCARen_US
dc.titleA molecular diagnosis method using real-time PCRfor quantification and detection of Fusarium oxysporumf. sp. cubense race 4en_US
dc.identifier.doi10.1007/s10658-012-0096-0zh_TW
item.languageiso639-1en_US-
item.grantfulltextnone-
item.fulltextno fulltext-
Appears in Collections:植物病理學系
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