Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/85209
DC FieldValueLanguage
dc.creatorChiou-Chu Suen_US
dc.creatorChung Jan Changen_US
dc.creatorChe-Ming Changen_US
dc.creatorHsien-Tzung Shihen_US
dc.creatorKuo-Ching Tzengen_US
dc.creatorFuh-Jyh Janen_US
dc.creatorChin-Wen Kaoen_US
dc.creatorWen-Ling Dengen_US
dc.date2013-06-21zh_TW
dc.date.accessioned2014-12-03T06:12:19Z-
dc.date.available2014-12-03T06:12:19Z-
dc.identifier.urihttp://hdl.handle.net/11455/85209-
dc.description.abstractCharacteristic symptoms of Pierce's disease (PD) in grapevines (Vitis vinifera L.) were observed in 2002 in the major grape production fields of central Taiwan. Disease severity in vineyards varied, and all investigated grape cultivars were affected. Diseased tissues were collected from fields for subsequent isolation and characterization of the causal agent of the disease (Xylella fastidiosa). Koch's postulates were fulfilled by artificially inoculating two purified PD bacteria to grape cultivars Kyoho, Honey Red and Golden Muscat. The inoculated plants developed typical leaf-scorching symptoms, and similar disease severity developed in the three cultivars from which the bacterium was readily re-isolated, proving that the leaf scorch of grapevines in Taiwan is caused by the fastidious X. fastidiosa. This confirmed PD of grapevines is also the first report from the Asian Continent. Phylogenetic analyses were performed by comparing the 16S rRNA gene and 16S-23S rRNA internal transcribed spacer region (16S-23S ITS) of 12 PD strains from Taiwan with the sequences of 13 X. fastidiosa strains from different hosts and different geographical areas. Results showed that the PD strains of Taiwan were closely related to the American X. fastidiosa grape strains but not to the pear strains of Taiwan, suggesting that the X. fastidiosa grape and pear strains of Taiwan may have evolved independently from each other.en_US
dc.format.medium期刊論文zh_TW
dc.language.isoen_USzh_TW
dc.relationJournal of Phytopathology, Volume 161, Issue 6, Page(s) 389-396.en_US
dc.relation.urihttp://onlinelibrary.wiley.com/doi/10.1111/jph.12075/abstract-
dc.subjectXylella fastidiosaen_US
dc.subjectgrapevineen_US
dc.subjectleaf scorch diseaseen_US
dc.subject16S rRNAen_US
dc.subject16S-23S ITSen_US
dc.subjectTaiwanen_US
dc.titlePierce’s Disease of Grapevines in Taiwan: Isolation, Cultivation and Pathogenicityof Xylella fastidiosaen_US
dc.identifier.doi10.1111/jph.12075zh_TW
item.languageiso639-1en_US-
item.grantfulltextnone-
item.fulltextno fulltext-
Appears in Collections:植物病理學系
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