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標題: Immunomodulation of phloretin by impairing dendritic cell activation and function
作者: Lin, Chi-Chen
Chu, Ching-Liang
Ng, Chin-Sheng
Lin, Ching-Yen
Chen, Der-Yuan
Pan, I-Hong
Huang, Kao-Jean
關鍵字: Animals;Cells, Cultured;Cytokines;Dendritic Cells;Immunologic Factors;Mice;Mice, Inbred C57BL;Phloretin;T-Lymphocytes;Toll-Like Receptor 4
Project: Food & function, Volume 5, Issue 5, Page(s) 997-1006.
Dietary compounds in fruits and vegetables have been shown to exert many biological activities. In addition to antioxidant effects, a number of flavonoids are able to modulate inflammatory responses. Here, we demonstrated that phloretin (PT), a natural dihydrochalcone found in many fruits, suppressed the activation and function of mouse dendritic cells (DCs). Phloretin disturbed the multiple intracellular signaling pathways in DCs induced by the Toll-like receptor 4 (TLR4) agonist lipopolysaccharide (LPS), including ROS, MAPKs (ERK, JNK, p38 MAPK), and NF-κB, and thereby reducing the production of inflammatory cytokines and chemokines. Phloretin also effectively suppressed the activation of DCs treated with different dosages of LPS or various TLR agonists. The LPS-induced DC maturation was attenuated by phloretin because the expression levels of the MHC class II and the co-stimulatory molecules were down-regulated, which then inhibited the LPS-stimulating DCs and the subsequent naïve T cell activation in a mixed lymphocyte reaction. Moreover, in vivo administration of phloretin suppressed the phenotypic maturation of the LPS-challenged splenic DCs and decreased the IFN-γ production from the activated CD4 T cells. Thus, we suggest that phloretin may potentially be an immunomodulator by impairing the activation and function of DCs and phloretin-contained fruits may be helpful in the improvement of inflammation and autoimmune diseases.
ISSN: 2042-650X
DOI: 10.1039/c3fo60548e
Appears in Collections:生物醫學研究所

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