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標題: Differential cytotoxic effects of gold nanoparticles in different mammalian cell lines
作者: Chueh, Pin Ju
Liang, Ruei-Yue
Lee, Yi-Hui
Zeng, Zih-Ming
Chuang, Show-Mei
關鍵字: AuNPs;Cell-impedance measurement;Cytotoxicity;Gold nanoparticles;In vitro assays;Animals;Apoptosis;Autophagy;Cell Proliferation;Cercopithecus aethiops;Colony-Forming Units Assay;Gold;Humans;Metal Nanoparticles;Mice;NIH 3T3 Cells;Oligonucleotide Array Sequence Analysis;Swine;Trypan Blue;Vero Cells;Toxicity Tests
Project: Journal of Hazardous Materials, Volume 264, Page(s) 303-312.
Gold nanoparticles (AuNPs) possess unique properties that have been exploited in several medical applications. However, a more comprehensive understanding of the environmental safety of AuNPs is imperative for use of these nanomaterials. Here, we describe the impacts of AuNPs in various mammalian cell models using an automatic and dye-free method for continuous monitoring of cell growth based on the measurement of cell impedance. Several well-established cytotoxicity assays were also used for comparison. AuNPs induced a concentration-dependent decrease in cell growth. This inhibitory effect was associated with apoptosis induction in Vero cells but not in MRC-5 or NIH3T3 cells. Interestingly, cDNA microarray analyses in MRC-5 cells supported the involvement of DNA damage and repair responses, cell-cycle regulation, and oxidative stress in AuNP-induced cytotoxicity and genotoxicity. Moreover, autophagy appeared to play a role in AuNPs-induced attenuation of cell growth in NIH3T3 cells. In this study, we present a comprehensive overview of AuNP-induced cytotoxicity in a variety of mammalian cell lines, comparing several cytotoxicity assays. Collectively, these assays offer convincing evidence of the cytotoxicity of AuNPs and support the value of a systematic approach for analyzing the toxicology of nanoparticles.
ISSN: 03043894
DOI: 10.1016/j.jhazmat.2013.11.031
Appears in Collections:生物醫學研究所

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