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標題: 紅花石斛無菌播種與幼苗生長
Seed Germination In Vitro and Plantlet Culture In Green House of Dendrobium victoria-reginae Loher. var. miyakei (Schltr.) Liu & Su
作者: Fitri Yunita
關鍵字: 無菌播種;活性炭;幼苗生長;seed germination;activated charcoal;seedling growth
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Dendrobium is a major floral crop around the world as a cut flower, medicinal plants, breeding, or ornamental plants. The present study had been completed to observe the seed germination and plantlets growth in medium containing different concentration of organic nitrogen, organic additives, activated charcoal in in vitro, and seedling growth in the green house. Liquid culture is suitable for germination, it also germinated faster than solid medium. On the other experiment, ? MS solid medium got the highest percentage compared to another media. In this study proved that organic nitrogen, tryptone has the highest value of height compared to peptone and casein. In the organic additive, coconut water give the best result for plantlets growth due to it has a more complete nutritions. activated charcoal has an important role in tissue culture for plant growth. This can be seen in the result that the best concentration for plant growth plantlet culture of Dendrobium victoria-reginae Loher var. miyakei is 2 g L-1 activated charcoal. Seedling growth is an important factor after culture in in vitro. Their initial growth in in vitro being followed by transplanting into green house or field. Based on this study, 0.5 g L-1 Hyponex-2 give the best result for the stem growth of Dendrobium victoria-reginae var. miyakei in green house.

紅花石斛為產於臺灣蘭嶼的原生蘭科植物,本文建立其種子發芽的無菌培育條件,篩檢活性炭促進瓶內小苗發育的合適濃度,以及溫室育苗的施肥濃度。紅花石斛的種子在靜置液體培養基中發芽比固體培養基更快,在另一實驗中1/4 MS固體培養基得到了最高比例的種子發芽率。紅花石斛種子發芽後1 cm高幼苗,培養在含為2 g L-1活性炭16週後可增加小苗的株高、葉數及根長。紅花石斛的瓶苗的移植到溫室栽培後,每二週於葉面噴施50 ml 的0.5 g L-1 花寶2號液體肥料,有助於增加小苗的莖粗及葉片寬度。
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