Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/89206
標題: 紅花石蒜雙鱗片繁殖及多倍體誘導
Twin scaling and polyploidy induction breeding of Lycoris radiata (L'Her.) Herb.
作者: Yi-Wei Lin
林奕幃
關鍵字: 紅花石蒜;雙鱗片;植物生長調節劑;椰子水;營養分析;多倍體誘導;twin-scales;plant growth regulator;coconut water;nutrient analysis;polyploidy induction breeding
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摘要: 
The present study establishes the fundamentals of the twin-scale propagation of Lycoris radiata and elaborates on the scale development stage, which is divided into the initial stage (scale prototype), bulblet development, rapid bulblet growth, rooting, and leaf development. After 12 weeks, the highest reproduction rate of 88.33% was observed at 25°C for 40 twin-scaled in addition to more roots and a longer root length. The survival rate was higher than 85% at both 20°C and 25°C.
The 200 and 500 mg/L NAA treatment yielded short and thick twin-scale roots but no bulblets, and more than 90% of the twin scales were moldy. The bulblets were subjected to different concentrations of BA at 8 weeks, and the highest survival rate of 69% was observed for the 200 mg/L treatment; this treatment also yielded the highest reproduction rate. Longer soaking time led to lower survival and reproduction rates. Furthermore, immersing the bulblets in different concentrations of coconut water and culturing them for 8 weeks yielded high survival and reproduction rates of 50% and 100% coconut water, and the reproduction rate was not affected by the time of immersion. Because of the high survival rate of twin-scaled, all survived scales that were transplanted to pots induced bulblets formation.
The content of mineral elements and the total soluble sugar of twin-scaled bulblets increased at the initial 2–4 weeks, suddenly dropped at 4–6 weeks, and were stable at 6–8 weeks. Starch was gradually assimilated.
Twin scales browning was observed after 4 days of shaking following colchicine treatment. After 12 weeks of culturing, most of bulblets were observed after they were subjected to control, 200, and 500 mg/L treatments and shaking for 1 day. The bulblets subjected to 1000 mg/L of colchicine treatment and shaking for 4 days also exhibited browning. Moreover, the DNA content revealed a twofold increase for one plant grown from the 500 mg/L of colchicine treatment and shaking for 1 day, as detected using flow cytometry, 500 ppm 1 day select 2, and found the longest stomata with 500 ppm 1 day 4. If mutagenic plants differ from normal plants, they should be observed for a long term.

本研究探討建立紅花石蒜雙鱗片扦插繁殖體系的基本條件與鱗片發育階段的資料,雙鱗片發育為小鱗莖的過程可分為鱗片原形期、小球發育期、小球快速生長期、小球發根期及小球出葉期,可與雙鱗片的營養期變化分別對應為初期、養分上升期、快速消耗期、平穩期。以插穗40片的處理有最高的小鱗莖形成率,達88.3%;在20℃及25℃下,存活率皆達85%以上;在25℃有最高的小鱗莖形成率,達52.5%,且小鱗莖性狀最佳,直徑3.2 mm,長度21.0 mm。培養八週後,NAA 200 及500 mg/L之處理未能誘導小鱗莖發育,只有短小且粗大之鱗片根,且約90%以上的鱗片發霉死亡;浸泡不同濃度的BA培養八週後,以200 mg/L處理之雙鱗片存活率最高,介於62%-69%,且小鱗莖形成率最高,介於57%-78%,其中每個濃度處理間,其浸泡時間越長,存活率越低,小鱗莖形成率亦越低。浸泡不同濃度椰子水培養八週後,50%及100%之處理鱗片存活率高達95%以上,小鱗莖形成率亦是以50%及100%之處理較高,50%之處理介於61%-73%,100%之處理介於64%-79%,浸泡時間不影響小鱗莖形成率。由於2014年的鱗片存活率高,移植時也將尚有活力之鱗片一同移植至培養介質中,經過一個生長季後,發現所有椰子水處理之鱗片都誘導出小鱗莖。
經秋水仙素震盪處理後,鱗片卷曲且薄膜脫離,震盪4天後的雙鱗片有褐化現象,扦插培養12週後,震盪1天之0 (對照組)、200 、500 mg/L處理長出的小鱗莖較多;震盪2天之0、200 、500 mg/L處理長出的小鱗莖則較少,且褐化嚴重,約60-70%;震盪4天與1000 mg/L的處理,則於培養時,鱗片皆褐化,未有任何組織長出。經流式細胞儀檢測發現500 mg/L的處理一天有兩株有DNA含量倍增的情形,分別為500 ppm 1day select 2及500 ppm 1day select 3,於500 ppm 1 day 4發現其氣孔長度最長62.34 μm。至於誘變株是否能有不同於其它正常植株的表現(如:開花性狀),尚須長期觀察。
URI: http://hdl.handle.net/11455/89206
其他識別: U0005-2207201513132900
Rights: 同意授權瀏覽/列印電子全文服務,2015-07-23起公開。
Appears in Collections:園藝學系

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