Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/89311
標題: Isolation and Identification of Major Antioxidative and Anti-Fatty Liver Phytocompounds from Leaf Extract of Rhododendron oldhamii Maxim.
金毛杜鵑葉部抽出物抗氧化及抗脂肪肝成分之分離與鑑定
作者: Ya-Ling Liu
劉雅玲
關鍵字: 金毛杜鵑;植化物;抗氧化;線上HPLC–DPPH;抗脂肪肝;R. oldhamii;phytochemical;antioxidant;online HPLC-DPPH;anti-fatty liver
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摘要: 
杜鵑屬植物為世界著名之觀賞植物,在民間亦作為藥用植物使用。然而,目
前對於金毛杜鵑之生物活性探討至今仍缺乏相關性研究。因此本試驗利用DPPH
自由基清除能力、超氧自由基清除能力試驗及還原力試驗等不同體外抗氧化評估
模式,配合總酚含量測定,並利用液相–液相將金毛杜鵑葉部甲醇抽出物劃分為:
正己烷可溶部、乙酸乙酯可溶部、正丁醇可溶部及水可溶部。而試驗結果發現,
各可溶部中以乙酸乙酯可溶部抗氧化效果最佳。而為進一步獲得乙酸乙酯可溶部
中具抗氧化活性潛能之化合物,本研究利用線上HPLC–DPPH 試驗對其進行快
速篩選,經分離及純化後共獲得(2R,3R)-Epicatechin(1)、(2R,3R)-Taxifolin(2)、
(2R,3R)-Astilbin(3)、Hyperoside(4)、Guaijaverin(5)以及Quercitrin(6)共
6 種主要具抗氧化潛力之化合物。而這些化合物中,又以化合物3 具有較高的含
量,於每克乙酸乙酯可溶部中其含量為130.8 mg。此外,DPPH 自由基清除能力
及超氧自由基清除能力係分別以化合物4 及6 表現最佳。另一方面,化合物4
及6 亦具有良好之抑制HepG2 細胞脂肪生成能力。而在C57BL/6 小鼠體內動物
模式中,補充金毛杜鵑EtOAc 可溶部能有效抑制脂肪肝形成,其作用機制包括
可減低膽固醇及脂肪合成相關基因SREBP-1 mRNA 表現,與增加脂肪代謝相關
基因CPT-1α、PPAR-α 及PPAR-γ mRNA 之表現。

Rhododendron is not only one of the most famous ornamental plants in the world,
but also it is a traditionally medicinal plant. However, the potential bioactivities of
Rhododendron oldhamii Maxim. have not yet been studied completely to date. In this
study, the methanolic crude extract of R. oldhamii were fractionated and divided into
n-hexane, EtOAc, BuOH, and water soluble fractions by liquid–liquid partition. In
addition, a number of in vitro assays including DPPH radical scavenging assay,
superoxide radical scavenging assay, reducing power assay, and total phenolic
contents were carried out to evaluate the antioxidant activities of n-hexane, EtOAc,
BuOH, and water soluble fractions. Among them, the EtOAc fraction exhibited the
best antioxidant activity. Base on bioactivity-guided isolation principle, the EtOAc
fraction was further monitored by an on-line HPLC-DPPH method for a rapid
screening of antioxidative phytochemicals. Six specific phytochemicals were isolated
and identified as (2R,3R)-epicatechin (1), (2R,3R)-taxifolin (2), (2R,3R)-astilbin (3),
hyperoside (4), guaijaverin (5), and quercitrin (6). Of these, compound 3 was found to
be the major bioactive compound, and it's contents were determined to be 130.8 mg
per gram of EtOAc fraction. Furthermore, compounds 4 and 6 exhibited the best
DPPH radical scavenging activity and superoxide radical scavenging activity,
respectively. On the other hand, compounds 4 and 6 also exhibited activity on
inhibiting HepG2 cell line lipogenesis. In C57BL/6 mice animal model, feeding of
EtOAc fraction from the mathanolic crude extract of R. oldhamii has an effective
anti-fatty liver capability, which also lowered cholesterol and fat synthesis related
iii
mRNA SREBP-1 expression, and increased metabolism related mRNA CPT-1α, PPAR-α and PPAR-γ expression.
URI: http://hdl.handle.net/11455/89311
其他識別: U0005-2108201515034300
Rights: 不同意授權瀏覽/列印電子全文服務
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