Please use this identifier to cite or link to this item:
標題: 水稻徒長病菌之生物特性:殘存能力及致病之進程
Characterization of Fusarium fujikuroi, with reference to the survival and the course of disease occurrence
作者: Chieh-Yu Lin
關鍵字: 水稻徒長病菌;水稻徒長病;土壤殘存;選擇性培養基;營養缺陷突變株;激勃素產量;Fusarium fujikuroi;bakanae disease;survival in soil;selective medium;nit mutant;gibberellin production
引用: 宇國勝、孫守恭。1977。稻苗徒長病傳染潛勢及發病潛伏期之研究。植保會刊19:245-250。 竹原利明, 國安克人. (1994). nit 変異菌株を用いたフザリウム病の発生生態の解明: I. Fusarium oxysporum の各分化型の nit 変異菌株の作成. 日本植物病理學會報, 60(6), 699-704. 伊藤誠哉, 木村某弥 (1931) 北海道農試研報27: 1-94. 余淑美。1980。不同寄主來源Fusarium moniliforme之生物學比較研究。碩士論文。國立中興大學。 林慶元、施錫彬、洪士程、徐保雄、陳治官、黃益田、劉清和、劉達修、蔣永正、蔣慕琰、鄭清煥、羅幹成。2007。水稻保護(下冊)。256-261。台北:行政院農業委員會動植物防疫檢疫局。 孫守恭。1978。稻苗徒長病菌(Gibberella fujikuroi)之生態及生殖。303-317。邱人璋編。水稻病蟲害:生態學與流行學。台北:中國農村復興聯合委員會。 陳又嘉。2014。臺灣水稻徒長病菌族群之遺傳組成、致病力及藥劑感受性分析。碩士論文。國立台灣大學。 許晴情。2013。水稻徒長病菌:開發鑑別性培養基、建立病害評估平台及探討土壤接種源之角色。碩士論文。國立中興大學。 張義璋。1973。稻苗徒長病菌有性世代及生態之研究。碩士論文。國立中興大學。 張義璋、孫守恭。1975。稻苗徒長病病原因之有性世代。中華農業研究24(1&2):11-19。 張義璋。2004。台灣水稻重要病害之生態及防治要領。農業試驗所特刊第111號,水稻健康管理研討會專集:75-101。 黑澤英一。1934。稻馬鹿苗病の病徵及病原菌就て。Transaction of the Natural History society of Fomasa. 18: 97. 黃介泓。2007。質譜術於豆類中激勃素及其代謝物之偵測。碩士論文。國立中興大學。 Abildgren, M. P., Lund, F., Thrane, U., & Elmholt, S. (1987). Czapek‐Dox agar containing iprodione and dicloran as a selective medium for the isolation of Fusarium species. Letters in Applied Microbiology, 5(4), 83-86. Andrews, S. and Pitt, J.I. (1986). Selective medium for the isolation of Fusarium species and dematiaceous hypomycetes from cereals. Applied and Environmental Microbiology, 51, 1235-1238. Anke, T. (1997). Fungal biotechnology. 193-199. Chapman & Hall. Bayman, P., & Cotty, P. J. (1991). Vegetative compatibility and genetic diversity in the Aspergillus flavus population of a single field. Canadian Journal of Botany, 69(8), 1707-1711. Bragulat, M. R., Martinez, E., Castella, G., & Caban, F. J. (2004). Selective efficacy of culture media recommended for isolation and enumeration of Fusarium spp. Journal of Food Protection®, 67(1), 207-211. Castellá, G., Bragulat, M.R., Rubiales, M.V. and Cabañes, F.J. (1997). Malachite green agar, a new selective medium for Fusarium spp. Mycopathologia, 137, 173-178. Coombe, B. G., D. Cohen', L. G. Paleg (1967). Barley Endosperm Bioassay for Gibberellins. I. Parameters of the Response System. Plant Physiology ,42(1), 105-112. Correll, J. C., Klittich, C. J. R. and Leslie, J. F. (1987) Nitrate nonutilizing mutants of Fusarium oxysporum and their use in vegetative compatibility tests. Phytopathology, 77, 1640- 1646 Gao, L., Sun, M. H., Liu, X. Z., & Che, Y. S. (2007). Effects of carbon concentration and carbon to nitrogen ratio on the growth and sporulation of several biocontrol fungi. Mycological Research, 111(1), 87-92. Hadar, E. S. T. H. E. R., Katan, J., & Katan, T. (1989). The use of nitrate-nonutilizing mutants and a selective medium for studies of pathogenic strains of Fusarium oxysporum. Plant Disease ,73, 800-803. Horn, B. W., & Dorner, J. W. (2009). Effect of nontoxigenic Aspergillus flavus and A. parasiticus on aflatoxin contamination of wounded peanut seeds inoculated with agricultural soil containing natural fungal populations. Biocontrol Science and Technology, 19(3), 249-262. Ikeda, A., Ueguchi-Tanaka, M., Sonoda, Y., Kitano, H., Koshioka, M., Futsuhara, Y., Matsuoka, M & Yamaguchi, J. (2001). slender rice, a constitutive gibberellin response mutant, is caused by a null mutation of the SLR1 gene, an ortholog of the height-regulating gene GAI/RGA/RHT/D8. The Plant Cell Online, 13(5), 999-1010. Ji, S. H., M. A. Gururani, J. W. Lee, B.-O. Ahn, S.-C. Chun (2013). Isolation and characterisation of a dwarf rice mutant exhibiting defective gibberellins biosynthesis. Plant Biology, 16(2), 428-439. Klittich, C. J. R., & Leslie, J. F. (1987). Nitrate nonutilizing mutants of Fusarium oxysporum and their use in vegetative compatibility tests. Phytopathology, 77, 1640-1646. Klittich, C., & Leslie, J. F. (1988). Nitrate reduction mutants of Fusarium moniliforme (gibberella fujikuroi). Genetics, 118(3), 417-423. Komada, H. (1975). Development of a selective medium for quantitative isolation of Fusarium oxysporum from natural soils. Review of Plant Protection Research, 8, 114-124. Kvas, M., Marasas, W. F. O., Wingfield, B. D., Wingfield, M. J., & Steenkamp, E. T. (2009). Diversity and evolution of Fusarium species in the Gibberella fujikuroi complex. Fungal Diversity, 34, 1-21. Liddell, C. M. & Burgess, L. W. (1985). Survival of Fusarium moniliforme at controlled temperature and relative humidity. Transactions of the British Mycological Society, 84(1), 121-130. Leslie & Summerell (2006), The Fusarium Laboratory Manual. 7-9, 31-42, 172-173. Blackwell Publishing. Lo, S. F., Yang, S. Y., Chen, K. T., Hsing, Y. I., Zeevaart, J. A., Chen, L. J., & Yu, S. M. (2008). A novel class of gibberellin 2-oxidases control semidwarfism, tillering, and root development in rice. The Plant Cell Online, 20(10), 2603-2618. Luo, Y., & TeBeest, D. O. (1999). Effect of Temperature and Dew Period on Infection of Northern Jointvetch by Wild-Type and Mutant Strains of Colletotrichum gloeosporioides f. sp. aeschynomene . Biological Control, 14(1), 1-6. Manzo, S. K., & Claflin, L. E. (1984). Survival of Fusarium moniliforme hyphae and conidia in grain Sorghum stalks. Plant Disease, 68(10), 866-867. Mauro, A., Battilani, P., Callicott, K. A., Giorni, P., Pietri, A., & Cotty, P. J. (2013). Structure of an Aspergillus flavus population from maize kernels in northern Italy. International Journal of Food Microbiology, 162(1), 1-7. McComb, A. J., D. J. Carr (1958). Evidence from a Dwarf Pea Bioassay for Naturally Occurring Gibberellins in the Growing Plant (Abstract). Nature, 181, 1548-1549. Nash, S.M. and Snyder, W.C. (1962). Quantitative estimations by plate counts of propagules of the bean root rot Fusarium spp. in field soils. Phytopathology, 52, 567-72. Nelson, P.E., Toussoun, T.A. and Marasas, W.F.O. (1983). Fusarium species: an illustrated manual for identification. Pennsylvania State University Press, University Park. Nishijima, T., Katsura, N. (1989). A modified micro-drop bioassay using dwarf rice for detection of femtomol quantities of gibberellins. Plant and Cell Physiology, 30(5), 623-627. Nyvall, R. F., & Kommedahl, T. (1966). Thickened hyphae a survival mechanism in Fusarium moniliforme. (Abstract) Phytopathology, 56, 893. Nyvall, R. F., & Kommedahl, T. (1968). Individual thickened hyphae as survival structures of Fusarium moniliforme in corn. Phytopathology, 58(1), 1704-1707. Ou, S. H. (1985). Rice diseases. IRRI. Paparu, P., Macleod, A., Dubois, T., Coyne, D., & Viljoen, A. (2009). Efficacy of chemical and fluorescent protein markers in studying plant colonization by endophytic non-pathogenic Fusarium oxysporum isolates. BioControl, 54(5), 709-722. Pavlista, A. D., D. K. Santra, D. D. Baltensperger. (2013). Bioassay of Winter Wheat for Gibberellic Acid Sensitivity. American Journal of Plant Sciences, 4, 2015-2022. Paleg, L. G. (1960). Physiological Effects of Gibberellic Acid. II. On Starch Hydrolyzing Enzymes of Barley Endosperm. Plant Physiology, 35(6), 902-906. Rekah, Y., Shtienberg, D., & Katan, J. (1999). Spatial distribution and temporal development of Fusarium crown and root rot of tomato and pathogen dissemination in field soil. Phytopathology, 89(9), 831-839. Ride, J. P., & Drysdale, R. B. (1972). A rapid method for the chemical estimation of filamentous fungi in plant tissue. Physiological Plant Pathology, 2(1), 7-15. Takehara, T., and Kuniyasu, K. (1997). Use of nitrate nonutilizing mutants in ecological studies of Fusarium diseases. IV. Seed transmission of Fusarium oxysporum f. sp. lycopersici race 2. (In Japanese, with English summary.) Bulletin of the National Agriculture Research Center (Japan), 26, 1-13. Takehara, T., Kuniyasu, K., Mori, M., & Hagiwara, H. (2003). Use of a nitrate-nonutilizing mutant and selective media to examine population dynamics of Fusarium oxysporum f. sp. spinaciae in soil. Phytopathology, 93(9), 1173-1181. Tio, M., Burgess, L. W., Nelson, P. E., & Toussoun, T. A. (1977). Techniques for the isolation, culture and preservation of the Fusaria. Australasian Plant Pathology, 6(1), 11-13. Van Wyk, P.S., Scholtz, D.J. and Los, O. (1986). A selective medium for the isolation of Fusarium spp. from soil debris. Phytophylactica, 18, 67-69. Whyte, P., Luckwill, L. C. (1966). A sensitive bioassay for gibberellins based on retardation of leaf senescence in Rumex obtusifolius (L.) (Abstract). Nature, 210, 1360. Zhang, H., Shen, W. B., & Xu, L. L. (2003). Effects of nitric oxide on the germination of wheat seeds and its reactive oxygen species metabolisms under osmotic stress. Acta Botanica Sinica, 45(8), 901-905.
Fusarium fujikuroi是造成水稻徒長病的病原菌,常造成水稻產量嚴重損失;一般認為稻種為最主要的感染來源,造成苗期的徒長,但是在田間有的徒長病徵是在分糵期或抽穗期出現,是否這些分糵期或抽穗期才出現的病徵來自受到感染的種子,或實際上為田間帶菌的土壤所造成。本研究希望能探討土壤接種源是否與分糵及抽穗期之病徵有關,以及種子受到感染後,不同的生育期是否有不同的病徵表現。本研究為了探討徒長病菌在土壤中的殘存能力,及是否足以造成田間的植株發病,首先改良出適合偵測土壤內徒長病菌之選擇性培養基,並設計標定之突變菌株,適合專一地追蹤人為接種之徒長病菌在自然環境下的變化,因此,本研究得以長時間追蹤在不同土壤成分及環境條件下徒長病菌之殘存與消長。實驗結果發現,土壤接種源確實能在田間造成病害,但比率極低;種子接種源造成秧苗100%發病後,同樣的植株病徵可能在不同時期消失,或在病徵消失一段時間後再度呈現,或自苗期始持續呈現徒長的病徵。而徒長病菌於土壤中殘存六個月後仍然保有高濃度的接種源,足以造成病害。最後,本研究希望能了解不同徒長病菌株在激勃素產量之相對能力,以不同菌株之培養液接種矮化品系之水稻後,作為生物分析激勃素產量的方法,結果發現此方法確實具可行性。

Fusarium fujikuroi is the causal agent of bakanae disease, usually resulting in severe yield loss. It has long been considered that the infection of seed is the major cause of this disease. Symptoms occur not only on seedling stage, but also on tillering and heading stages. It is very likely that the symptoms of tillering and heading stage are from soil inoculum. The aim of this study is to investigate the correlation between soil inoculums and disease occurrence on tillering and heading stages, and to realize the disease process after seeds were infected. To evaluate the ability of survival of pathogens, modified selective medium was established, and marked nit mutants were developed. The results suggest that the soil inoculum do have the ability to cause disease, although the incidence is low. Inoculated seeds lead to 100% diseased seedlings, among which some may appear healthy in later stage (some of them may appear diseased again), some may stay diseased continuously. With regard to the production of gibberellins of different isolates, a bioassay method was designed. A dwarf mutant line of rice was used to quantify the production of gibberellins.
Rights: 同意授權瀏覽/列印電子全文服務,2017-08-30起公開。
Appears in Collections:植物病理學系

Files in This Item:
File SizeFormat Existing users please Login
nchu-104-7101035114-1.pdf3.24 MBAdobe PDFThis file is only available in the university internal network    Request a copy
Show full item record

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.