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|標題:||Response of Sucrose-Starvation Induced Antioxidant Defense System in Rice Suspension Cells
|關鍵字:||sucrose starvation;rice suspension cells (Tainung 67, Oryza sativa L.);antioxidative defense system;oxidative stress;aquaporin;缺糖;水稻台農67號 (Oryza sativa L. cv. Tainung 67) 懸浮細胞;抗氧化防禦系統;氧化逆境;水通道蛋白||引用:||Anderson, M.D., T.K. Prasad and C.R. Stewart. 1995. Changes in isozyme profiles of catalase, peroxidase, and glutathione reductase during acclimation to chilling in mesocotyls of maize seedlings. Plant Physiol. 109: 1247-1257. Arora, A., R.K. Sairam and G.C. Sriuastava. 2002. Oxidative stress and antioxidative system in plants. Curr. Sci. 82: 1227-1238. Asada, K. 2006. Production and scavenging of reactive oxygen species in chloroplasts and their functions. Plant Physiol. 141: 391-396. Bajji, M., J.M. Kinet and S. Lutts. 2001. The use of the electrolyte leakage method for assessing cell membrane stability as a water stress tolerance test in durum wheat. Plant Growth Regul. 00: 1-10. Beauchamp, C. and I. Fridovich. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44: 276-287. Becana, M., J.F. Moran and I. Iturbe-Ormaetxe. 1998. Iron-dependent oxygen free radical generation in plants subjected to environmental stress: toxicity and antioxidant protection. Plant Soil 201: 137-147. Bhattacharjee, S. 2005. Reactive oxygen species and oxidative burst: roles in stress, senescence and signal transduction in plant. Curr. Sci. 89: 1113-1121. Bienert, G.P., A.L.B. Moller, K.A. Kristiansen, A. Schulz, I.M. Moller, J.K. Schjoerring and T.P. Jahn. 2007. Speci?c aquaporins facilitate the diffusion of hydrogen peroxide across membranes. J. Biol. Chem. 282: 1183-1192. Bienert, G.P. and F. Chaumont. 2014. Aquaporin-facilitated transmembrane diffusion of hydrogen peroxide. Biochim. Biophys. Acta. 1840: 1596-1604. Boehm, R. 2007. Bioproduction of therapeutic proteins in the 21st century and the role of plants and plant cells as production platforms. Ann. NY. Acad. Sci. 1102: 121-134. Brouquisse, R., F. James, A. Pradet and P. Raymond. 1992. Asparagine metabolism and nitrogen distribution during protein degradation in sugar-starved maize root tips. Planta 188: 384-395. Chen, M.H., L.F. Liu, Y.R. Chen, H.K. Wu and S.M. Yu. 1994. Expression of α-amylases, carbohydrate metabolism, and autophagy in cultured rice cells is coordinately regulated by sugar nutrient. Plant J. 6: 625-636. Chung, B.C., S.Y. Lee, S.A. Oh, T.H. Rhew, H.G. Nam and C.H. Lee. 1997. The promoter activity of sen1, a senescence-associated gene of Arabidopsis, is repressed by sugars. J. Plant Physiol. 151: 339-345. Contento, A.L. and D.C. Bassham. 2010. Increase in catalase-3 activity as a response to use of alternative catabolic substrates during sucrose starvation. Plant Physiol. Biochem. 48: 232-238. Contento, A.L., S.J. Kim and D.C. Bassham. 2004. Transcriptome profiling of the response of Arabidopsis suspension culture cells to Suc starvation. Am. Soc. Plant Biol. 135: 2330 -2347. Cou?e, I., J. Murielle, J.P. Carde, R. Brouquisse, P. Raymond and A. Pradet. 1992. Effects of glucose starvation on mitochondrial subpopulations in the meristematic and submeristematic regions of maize root. Plant Physiol. 100: 1891-1900. Dynowski, M., G. Schaaf, D. Loque, O. Moran and U. Ludewig. 2008. Plant plasma membrane water channels conduct the signaling molecule H2O2. Biochem. J. 414: 53-61. Elstner, E.F. 1991. Mechanism of oxygen activation in different compartments of plant cells. In Active Oxygen/Oxidative Stress and Plant Metabolism (ed Pell E.J. and K.L Steffen). Am. Soci. Plant Physiol. Rockville, MD, USA. 13-25. Foyer, C., M. Lelandais, C. Galap and K.J. Kunert. 1991. Effects of elevated cytosolic glutathione reductase activity on the cellular glutathione pool and photosynthesis in leaves under normal and stress conditions. Plant Physiol. 97: 863-872. Foyer, C.H. and G. Noctor. 2003. Redox sensing and signalling associated with reactive oxygen in chloroplasts, peroxisomes and mitochondria. Physiol. Plant 119: 355-364. Foster, J.G. and J.L. Hess. 1980. Responses of superoxide dismutase and glutathione reductase activities in cotton leaf tissue exposed to an atmosphere enriched in oxygen. Plant Physiol. 66: 482-487. Fridovich, I. 1995. Superoxide radical and superoxide dismutases. Annu. Rev. Biochem. 64: 97-112. Gill, S.S. and N. Tuteja. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol. Biochem. 909-930. Heath, R.L. and L. Packer. 1968. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 125: 189-198. Hellwig, S., J. Drossard, R.M. Twyman and R. Fischer. 2004. Plant cell cultures for the production of recombinant proteins. Nat. Biotechnol. 22: 1415-1422. Hooijmaijers, C., J.Y. Rhee, K.J. Kwak, G.C. Chung, T. Horie, M. Katsuhara and H. Kang. 2012. Hydrogen peroxide permeability of plasma membrane aquaporins of Arabidopsis thaliana. J. Plant Res. 125: 147-153. Huang, L.F., Y.K. Liu, C.A. Lu, S.L. Hsieh and S.M. Yu. 2005. Production of human serum albumin by sugar starvation induced promoter and rice cell culture. Transgenic Res. 14: 569-581. Huang, T.K. and K.A. McDonald. 2012. Bioreactor systems for in vitro production of foreign proteins using plant cell cultures. Biotechnol. Adv. 30: 398-409. Huang, J., T.D. Sutliff, L. Wu, S. Nandi, K. Benge, M. Terashima, A.H. Ralston, W. Drohan, N. Huang and R.L. Rodriguez. 2001. Expression and purification of functional human alpha-1-Antitrypsin from cultured plant cells. Biotechnol. Prog. 17: 126-133. James, E. and J.M. Lee. 2001. The production of foreign proteins from genetically modified plant cells. Adv. Biochem. Eng./Biotechnol. 72: 127-156. Jana, S. and M.A. Choudhuri. 1981. Glycolate metabolism of three submerged aquatic angiosperm during aging. Aquat. Bot. 12: 45-354. Johar, S., A.C. Cave, A. Narayanapanicker, D.J. Grieve and A.M. Shah. 2006. Aldosterone mediates angiotensin II-induced interstitial cardiac ?brosis via a Nox2-containing NADPH oxidase. The FASEB J. 20: 1546-1548. Karg, S.R. and P.T. Kallio. 2009. The production of biopharmaceuticals in plant systems. Biotechnol. Adv. 27: 879-894. Karpinski, S., C. Escobar, B. Karpinska, G. Creissen and P.M. Mullineaux. 1997. Photosynthetic electron transport regulates the expression of cytosolic ascorbate peroxidase genes in Arabidopsis during excess light stress. Plant Cell 9: 627-640. Katsuhara, M., S. Sasano, T. Horie, T. Matsumoto, J. Rhee and M. Shibasaka. 2014. Functional and molecular characteristics of rice and barley NIP aquaporins transporting water, hydrogen peroxide and arsenite. Plant Biotechnol. DOI: 10.5511/plantbiotechnology.14.0421a Keunen, E., T. Remans, S. Bohler, J. Vangronsveld and A. Cuypers. 2011. Metal-induced oxidative stress and plant mitochondria. Int. J. Mol. Sci. 12: 6894-6918. Leader, B., Q.J. Baca and D.E. Golan. 2008. Protein therapeutics: a summary and pharmacological classi?cation. Nat. Rev. Drug Discov. 7: 21-39. Li., Z. G., M. Gong, H. Xie, L. Yang and J. Li. 2012. Hydrogen sul?de donor sodium hydrosul?de-induced heat tolerance in tobacco (Nicotiana tabacum L.) suspension cultured cells and involvement of Ca2+ and calmodulin. Plant Sci. 195-186: 185-189. Liu, Y., H. Jiang, Z. Zhao and L. An. 2011. Abscisic acid is involved in brassinosteroids-induced chilling tolerance in the suspension cultured cells from Chorispora bungeana. J. Plant Physiol. 168: 853-862. Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 7: 405-410. Mittler, R. and B.A. Zilinskas. 1993. Detection of ascorbate peroxidase activity in native gels by inhibition of the ascorbate-dependent reduction of nitroblue tetrazolium. Anal. Biochem. 212: 540-546. Morkunas, I., M. Garnczarska, W. Bednarski, W. Ratajczak and S. Waplak. 2003. Metabolic and ultrastructural responses of lupine embryo axes to sugar starvation. J. Plant Physiol. 160: 311-319. Morkunas, I., S. Borek, M. Formela and L. Ratajczak. 2012. Plant responses to sugar starvation. In: Chang CF, editor. Carbohydrates-comprehensive studies on glycobiology and glycotechnology. New York NY: InTech: 409-438. Morkunas, I. and W. Bednarski. 2008. Fusarium oxysporum induced oxidative stress and antioxidative defenses of yellow lupine embryo axes with different level of sugars. J. Plant Physiol. 165: 262-277. Munn?-Bosch, S. and L. Alegre. 2002. Plant aging increases oxidative stress in chloroplasts. Planta 214: 608-615. Mustafa, M.G. 1990. Biochemical basis of ozone toxicity. Free Radical Bio. Med. 9: 245-265. Nakano, Y. and K. Asada. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 22: 867-880. Noctor, G., S. Veljovic-Jovanovic, S. Driscoll, L. Novitskaya and C.H. Foyer. 2002. Drought and oxidative load in the leaves of C3 plants: a predominant role for photorespiration? Ann. Bot.-London 89: 841-850. Park, C.I., S.J. Lee, S.H. Kang, H.S. Jung, D.I. Kim and S.M. Lim. 2010. Fed-batch cultivation of transgenic rice cells for the production of hCTLA4Ig using concentrated amino acids. Process Biochem. 45: 67-74. Parrott, D., L. Yang, L. Shama and A.M. Fischer. 2005. Senescence is accelerated, and several proteases are induced by carbon 'feast' conditions in barley (Hordeum vulgare L.) leaves. Planta 222: 989-1000. Plaxton, W.C. 1996. The organization and regulation of plant glycolysis. Annu. Rev. Plant Physiol. Plant Mol. Biol. 47: 185-214. Pourtau, N., R. Jennings, E. Pelzer, J. Pallas and A. Wingler. 2006. Effect of sugar-induced senescence on gene expression and implications for the regulation of senescence in Arabidopsis. Planta 224: 556-568. Reinhound, P. J., E. W. M. Schrijnemakers, F. Van Iren and J. W. Kijne. 1995. Vitrification and a heat-shoot treatment improve cryopreservation of tobacco cell suspension compared to two-step freezing. Plant Cell Tissue Organ Cult. 42:261-267. Renu, K.C. 2011. Chloroplasts and mitochondrial have multiple heat tolerant isozymes of SOD and APX in leaf and inflorescence in Chenopodium album. India Biochem. Biophys. Res. Commun. 412: 522-525. Rose, T.L., L. Bonneau, C. Der, D. Marty-Mazars and F. Marty. 2006. Starvation-induced expression of autophagy-related genes in Arabidopsis. Biol. Cell 98: 53-67. Rybicki, E.P. 2010. Plant-made vaccines for humans and animals. Plant Biotechnol. J. 8: 620-637. Streat?eld, S.J. 2007. Approaches to achieve high-level heterologous protein production in plants. Plant Biotechnol. J. 5: 2-15. Sagi, M. and R. Fluhr. 2006. Production of reactive oxygen species by plant NADPH oxidases. Plant Physiol. 141: 336-340. Sheu, J.J., T.S. Yu, W.F. Tong and S.M. Yu. 1996. Carbohydrate starvation stimulates di?erential expression of rice alpha-amylase genes that is modulated through complicated transcriptional and posttranscriptional processes. J. Biol. Chem. 271: 26998–27004. Shih, S.M.H. and P.M. Doran. 2009. Foreign protein production using plant cell and organ cultures: advantages and limitations. Biotechnol. Adv. 27: 1036-1042. ?lesak, I, M. Libik, B. Karpinska, S. Karpinski and Z. Miszalski. 2007. The role of hydrogen peroxide in regulation of plant metabolism and cellular signalling in response to environmental stresses. Acta Biochim. Pol. 54: 39-50. Sousa-Lopes, A., F. Antunes, L. Cyrne and H.S. Marinho. 2004. Decreased cellular permeability to H2O2 protects Saccharomyces cerevisiae cells in stationary phase against oxidative stress. FEBS Lett. 578: 152-156. Su, W.W. and K.T. Lee. 2007. Plant cell and hairy-root cultures-process characteristics, products, and application. In: Yang ST (ed) Bioprocessing for value-added products from renewable resources. Elsevier Science, Amsterdam, pp 263-292. Temple, M.D., G.C. Perrone and I.W. Dawes. 2005. Complex cellular responses to reactive oxygen species. Trends Cell Biol. 15: 319-326. Terashima, M., Y. Murai, M. Kawamura, S. Nakanishi, T. Stoltz, L. Chen, W. Drohan, R.L. Rodriguez and S. Katoh. 1999. Production of functional human alpha(1)-antitrypsin by plant cell culture. Appl. Microbiol. Biotechnol. 52: 516-523. Tyerman, S.D., C.M. Niemietz, and H. Bramley. 2002. Plant aquaporins: multifunctional water and solute channels with expanding roles. Plant Cell Environ. 25: 173-194. Wang, H.J., A.R. Wan, C.M. Hsu, K.W. Lee, S.M. Yu and G.Y. Jauh. 2007. Transcriptomic adaptations in rice suspension cells under sucrose starvation. Plant Mol. Biol. 63: 441-463. Weather, P.J., M.J. Towler and J. Xu. 2010. Bench to batch: advances in plant cell culture for producing useful products. Appl. Microbiol. Biotechnol. 85: 1339-1351. Wise, R.R. and A.W. Naylor. 1987. Chilling-enhanced photooxidation. Evidence for role of singlet oxygen and endogenous antioxidants. Plant Physiol. 83: 278-282. Wong, H.L., R. Pinontoan, K. Hayashi, R. Tabata, T. Yaeno, K. Hasegawa, C. Kojima, H. Yoshioka, K. Iba, T. Kawasaki and K. Shimamoto. 2007. Regulation of rice NADPH oxidase by binding of Rac GTPase to Its N-terminal extension. The Plant Cell 19: 4022-4034. Woodnutt, G., B. Violand and M. North. 2008. Advances in protein therapeutics. Curr. Opin. Drug Discov. Dev. 11: 754-761. Wu, B. and E. Beitz. 2007. Aquaporins with selectivity for unconventional permeants. Cell Mol. Life Sci. 64: 2413-2421. Xu, J., X. Ge and M.C. Dolan. 2011. Towards high-yield production of pharmaceutical proteins with plant cell suspension cultures. Biotechnol. Adv. 29: 278-299. Xu, J.F., L. Tan, D.T.A. Lamport, A.M. Showalter and M.J. Kieliszewski. 2008. The O-Hyp glycosylation code in tobacco and Arabidopsis and a proposed role of Hyp-glycans in secretion. Phytochemistry 69: 1631-1640. Yakimova, E.T., V.M. Kapchina-Toteva, L.J. Laarhoven, F.M. Harren and E.J. Woltering. 2006. Involvement of ethylene and lipid signaling in cadmium-induced programmed cell death in tomato suspension cells. Plant Physiol. Biochem. 44: 581-589. Yu, S.M. 1999. Cellular and genetic responses of plants to sugar starvation. Plant Physiol. 121: 687-693. Yu, S.M., Y.H. Kuo, G. Sheu, Y.J. Sheu and L.F. Liu. 1991. Metabolic derepression of alpha-amylase gene expression in suspension-cultured cells of rice. J. Biol. Chem. 266: 21131–21137.||摘要:||
本論文主要探討缺糖對水稻台農67號 (Oryza sativa L. cv. Tainung 67) 懸浮細胞抗氧化防禦系統變化之影響。缺糖明顯抑制懸浮細胞鮮重和乾重，且顯著降低細胞內蛋白質含量與細胞活力。雖然在細胞內無MDA與H2O2增加的現象，但細胞外 (培養基) 發現有大量H2O2累積，而H2O2累積可能會造成氧化逆境的發生。抗氧化酵素中，SOD與CAT活性顯著受缺糖誘導而提升，另外，NADPH oxidase活性也有增加，顯示當NADPH oxidase產生O2·-時，SOD會將其轉換為H2O2，而部分的H2O2會被CAT代謝成水。此外，近年來研究指出H2O2可經由水通道蛋白進出細胞，在水稻懸浮細胞缺糖處理時，亦有發現部分水通道蛋白在缺糖6小時表現量有提升的現象，這些結果說明缺糖會導致懸浮細胞產生氧化逆境，雖然細胞內SOD與CAT活性提升可減緩氧化逆境，但H2O2仍可能藉由水通道蛋白運輸至細胞外，進而造成細胞氧化傷害。
This study focuses on the effect of sugar starvation on the response of antioxidative defense system in rice suspension cells (Tainung 67, Oryza sativa L.). Sugar starvation decreased fresh weight and dry weight of suspension cells, as well as protein content and cell viability. No significant changes of MDA and H2O2 were detected in the cells, however, notable accumulation of H2O2 was found in medium. Activity of SOD and CAT was increased under sugar starvation in suspension cells. In addition, activity of NADPH oxidase was increased indicating that O2·- generated by NADPH oxidase would be converted into H2O2 by SOD, and levels of H2O2 would be converted into H2O by CAT in the cells. It has been known that H2O2 could get in or out of cells via aquaporin (AQP). Under the treatment of sugar starvation, content of some AQP increased after 6 hours. These results proved that sugar starvation would lead to a decrease of activity in both protein content and cell viability of suspension cell from rice. Besides sugar starvation would lead to oxidative stress, H2O2 could be transferred out of the cell via AQP and resulted in oxidative damage to cells, though increase in activity of SOD and CAT can relief oxidative stress.
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