Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/89502
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dc.contributor鄭雅銘zh_TW
dc.contributorYa-Ming Chengen_US
dc.contributor.author簡鈺倫zh_TW
dc.contributor.authorYu-Lun Chienen_US
dc.contributor.other農藝學系所zh_TW
dc.date2014zh_TW
dc.date.accessioned2015-12-07T08:42:24Z-
dc.identifierU0005-2702201412134200zh_TW
dc.identifier.citation林倩宇。2013。利用玉米B-10L易位染色體進行B染色體斷裂點及CL-repeat之相互定位。碩士論文。台中:國立中興大學農藝學系。 高國維。2013。實體定位與結構分析四個玉米B染色體專一性RAPD標誌。碩士論文。台中:國立中興大學農藝學系。 Alfenito, M.R. and J.A. Birchler. 1993. Molecular characterization of a maize B chromosome centric sequence. Genetics 135: 589-597. Beckett, J.B. 1978. B-A translocations in maize I. Use in locating genes by chromosome arms. J. Hered. 69: 27-36. Beckett, J.B. 1991. Cytogenetic, genetic and plant breeding applications of B-A translocations in maize. pp. 493-529 in Chromosome engineering in plants: Genetics, Breeding, Evolution. Part A, edited by P.K. Gupta and T. Tsuchiya. Elsevier, Amsterdam. Bouck, A.C., R. Peeler, M.L. Arnold and S.R. Wessler. 2005. Genetic mapping of species boundaries in Louisiana irises using IRRE retrotransposon display markers. Genetics 171: 1289-1303. Carlson, W.R. 1969. Factors affecting preferential fertilization in maize. Genetics 62: 543-554. Carlson, W.R. and T.S. Chou. 1981. B chromosome nondisjunction in corn: control by factors near the centromere. Genetics 97: 379-389. Carlson, W.R. and R.R. Roseman. 1992. A new property of the maize B chromosome. Genetics 131: 211-223. Cheng, Y.M. 2010. Evolution of the heterochromatic regions on maize B long arm based on the sequence structure of CL-repeat variants. Chromosome Res. 18: 605-619. Cheng, Y.M. and B.Y. Lin. 2003. Cloning and characterization of maize B chromosome sequences derived from microdissection. Genetics 164: 299-310. Cheng, Y.M. and B.Y. Lin. 2004. Molecular organization of large fragments in the maize B chromosome: indication of a novel repeat. Genetics 166: 1947-1961. Chilton, M.D. and B.J. McCarthy. 1973. DNA from maize with and without B chromosomes: a comparative study. Genetics 74: 605-614. Gourmet, C. and A.L. Rayburn. 1996. Identification of RAPD markers associated with the presence of B chromosomes in maize. Heredity 77: 240-244. Jones, N. and A. Houben. 2003. 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Regional control of nondisjunction of the B chromosome in maize. Genetics 90: 613-627. Lin, B.Y. 1979. Two new B-10 translocations involved in the control of nondisjunction of the B chromosome in maize. Genetics 92: 931-945. Lin, B.Y. and H.P. Chou. 1997. Physical mapping of four RAPDs in the B chromosome of maize. Theor. Appl. Genet. 94: 534-538. Longley, A.E. 1927. Supernumerary chromosomes in Zea mays. J. Agric. Res. 35: 769-784. Masonbrink, R.E. and J.A. Birchler. 2010. Sporophytic nondisjunction of the maize B chromosome at high copy numbers. J. Genet. Genom. 37: 79-84. McClintock, B. 1933. The association of non-homologous parts of chromosomes in the mid-prophase of meiosis in Zea mays.Z. Zellf. Mik. Anat. 19: 191-237. Peng, S.F., Y.P. Lin and B.Y. Lin. 2005. Characterization of AFLP sequences from regions of maize B chromosome defined by 12 B-10L translocations. Genetics 169: 375-388. Randolph, L.F. 1928. Types of supernumerary chromosomes in maize. Anat. Rec. 41:102. Randolph, L. 1941. Genetic characteristics of the B chromosomes in maize. Genetics 26:608-631. Roman, H. 1947. Mitotic nondisjunction in the case of interchanges involving the B-type chromosome in maize. Genetics 32: 391-409. Roman, H. 1948. Directed fertilization in maize. Proc. Natl. Acad. Sci. USA 34: 36-42. Saghai-Maroof, M.A., K.M, Soliman, R.A. Jorgensen and R.W. Allard. 1984. Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc. Natl. Acad. Sci. USA 81: 8014-8018. Stark, E.A., I. Connerton, S.T. Bennett, S.R. Barnes, J.S. Parker and J.W. Forster. 1996. Molecular analysis of the structure of the maize B-chromosome. Chromosome Res. 4: 15-23. Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. Lee, M. Hornes, A. Friters, J. Pot, J. Paleman and M. Kuiper. 1995. AFLP: A new technique for DNA fingerprinting. Nucleic Acids Res. 23: 4407-4414. Ward, E.J. 1973. Nondisjunction: localization of the controlling site in the maize B chromosome. Genetics 73: 387-391. Williams, J.G., A.R. Kubelik, K.J. Livak, J.A. Rafalski and S.V. Tingey. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18: 6531-6535.zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/89502-
dc.description.abstract為了獲得大量玉米B染色體專一性的分子標誌,利用B染色體專一性序列CL-repeat廣泛分佈在B染色體的特性,並參考鳶尾逆轉位子顯示技術,建立玉米B染色體CL-repeat顯示技術。總共獲得26個B染色體專一性標誌,並利用5個B-10L易位染色體進行標誌定位,再利用13個位於異染色質區的B-10L易位染色體進行相互定位,藉以確定易位染色體斷裂點的位置並精細定位各個CL-repeat顯示標誌。將26個標誌進行選殖與序列分析,依序列特性可分成CL-repeat序列標誌、B染色體中節序列標誌、同時帶有CL-repeat與B染色體中節序列標誌及未知序列標誌此4大類。接著將1個B染色體中節序列標誌、2個同時帶有CL-repeat與B染色體中節序列標誌及2個未知序列標誌轉換成SCAR (sequence characterized amplified region)標誌,並利用B-10L易位染色體進行定位,其中帶B染色體中節序列之3i-08-SCAR標誌出現在非預期的區域,將不同區域的標誌進行選殖與定序,並製成無根樹演化圖,以探討玉米B染色體中節序列在中節與遠端異染色質區的差異。另外,以限制酵素HindIII進行CL-repeat顯示技術,期望獲得更多不同的B染色體專一性的CL-repeat顯示標誌。zh_TW
dc.description.abstractFor isolating a large number of maize B-chromosome specific molecular markers, we used the B-chromosome specific sequence, CL-repeat which are widely distributed on the B-chromosome, and referred to the IRRE (iris retroelement) retrotransposon display technique to establish the CL-repeat display technique of maize B-chromosome. A total of 26 B-chromosome specific markers were obtained and mapped by five B-10L translocations. Additonal 13 B-10L translocations with breakpoints on the distal heterochromatic region were used to verify breakpoints of these translocations and to finely map each CL-repeat display marker. All of the 26 markers were cloned and sequenced. On the basis of sequence characteristics, those sequence could be divide into four categories: markers with CL-repeat sequence, markers with B-centromere sequence, markers with CL-repeat and B-centromere sequences, and markers with unknown sequence. Subsequently, one marker with B-centromere sequence, two markers with CL-repeat and B-centromere sequences, and two markers with unknown sequence were selected to convert into SCAR (sequence characterized amplified region) markers, and mapped by B-10L translocations. Among these five markers, the 3i-08-SCAR marker with B-centromere sequence were mapped to unexpected regions. Those unexpected SCAR markers were cloned and sequenced from different regions, and used to constructed an unrooted evolution tree for exploring the differentiation of maize B-centromere sequences between the B-centromere and the distal heterochromatin region. Furthermore, we used restriction enzyme HindIII to perform the CL-repeat display technique and hoped to get more different B-chromosome specific CL-repeat display markers.en_US
dc.description.tableofcontents目次 摘要 i Abstract ii 目次 iv 表目次 vi 圖目次 vii 第一章、前言 1 第二章、前人研究 2 一、B染色體概論 2 二、玉米B染色體的細胞學形態 2 三、玉米B染色體的遺傳特性 3 四、玉米B染色體專一性序列 5 五、玉米B-A易位染色體之研究 7 六、玉米B染色體的分子標誌 9 七、玉米B染色體的分子結構 10 八、顯示技術的建立與應用 12 第三章、材料與方法 14 一、玉米材料 14 二、根尖有絲分裂中期染色體數目計數 14 三、玉米基因組DNA的萃取 14 四、CL-repeat顯示技術 15 (一)限制酵素剪切 15 (二)銜接子的黏合 16 (三)聚合酶鏈鎖反應 16 (四)聚丙烯醯胺膠體電泳分析 16 (五)篩選玉米B染色體專一標誌 17 五、CL-repeat顯示標誌的擴充 18 六、選殖B染色體專一性標誌與序列分析 18 (一)B染色體專一性標誌回收與PCR再增幅 18 (二)黏合反應(ligation) 18 (三)勝任細胞的製備 19 (四)轉型 19 (五)菌株(colony)PCR 19 (六)限制酵素剪切菌落PCR產物 20 (七)質體DNA的萃取 20 (八)核酸定序 21 (九)序列分析 21 七、序列特異性增幅區間(sequence characterized amplified region, SCAR)標誌之轉換 22 (一)SCAR分子標誌引子設計 22 (二)SCAR-PCR 22 第四章、結果 23 一、建立CL-repeat顯示技術 23 二、篩選玉米B染色體專一性CL-repeat顯示標誌 24 三、利用B-10L易位染色體初步定位CL-repeat顯示標誌 24 四、利用CL-repeat顯示標誌定位斷裂點位於遠端異染色質區的B-10L易位染色體 25 五、B染色體專一性CL-repeat顯示標誌序列分析 26 六、CL-repeat 顯示技術的擴充 27 七、SCAR標誌之轉換 27 八、標誌3i-13與3i-08在B染色體不同位置之比較 29 第五章、討論 31 一、CL-repeat顯示技術的建立過程 31 二、玉米B染色體中節序列的變異 32 三、CL-repeat顯示標誌與易位染色體相互實體定位 34 四、CL-repeat顯示技術的未來應用 36 五、存在於BS-PE區域之CL-repeat顯示標誌的解釋 37 第六章、參考文獻 38 表目次 表一、CL-repeat 顯示技術使用的引子序列 44 表二、CL-repeat顯示標誌 45 表三、以5個B-10L易位染色體定位26個CL-repeat展示 標誌 46 表四、以13個B-10L易位染色體定位26個CL-repeat展示 標誌 47 表五、SCAR-PCR使用的引子序列 49 圖目次 圖一、玉米有絲分裂中期根尖染色體 50 圖二、13個B-10L易位染色體B染色體斷裂點的模式圖 51 圖三、CL-repeat顯示技術流程圖 52 圖四、利用HindIII進行CL-repeat顯示技術 53 圖五、篩選B染色體專一性CL-repeat顯示標誌 54 圖六、利用B-10L易位染色體進行初步定位CL-repeat顯示 標誌 55 圖七、以5個B-10L易位染色體定位26個CL-repeat顯示 標誌模式圖 56 圖八、利用位於遠端異染色質區的B-10L易位染色體定位 CL-repeat顯示標誌。 57 圖九、以13個B-10L易位染色體定位26個CL-repeat顯示 標誌模式圖 58 圖十、26個CL-repeat顯示標誌的序列分析 59 圖十一、利用HindIII於CL-repeat顯示技術 60 圖十二、利用B-10L易位染色體定位3i-13-SCAR標誌 61 圖十三、利用B-10L易位染色體定位5i-02-SCAR標誌 62 圖十四、利用B-10L易位染色體定位5i-03-SCAR標誌 63 圖十五、3i-02標誌SCAR引子篩選 64 圖十六、以6個B-10L易位染色體定位標誌3i-08-SCAR 65 圖十七、以6個B-10L易位染色體定位3i-08-SCAR標誌 模式圖 66 圖十八、不同B染色體區域的標誌3i-13-SCAR(玉米B染 色體中節序列)的核苷酸序列分析 67 圖十九、以30個來自玉米B染色體4個不同區域的玉米 B染色體中節序列建構之無根演化樹 69 圖二十、不同區域的標誌3i-08-SCAR的核苷酸序列分析 70 圖二十一、以8個來自玉米B染色體2個不同區域的標誌 3i-08-SCAR建構之無根演化樹 71 圖二十二、B-10L易位染色體在B染色體上斷裂點 72zh_TW
dc.language.isozh_TWzh_TW
dc.rights不同意授權瀏覽/列印電子全文服務zh_TW
dc.subjectmaizeen_US
dc.subjectB-chromosomeen_US
dc.subjectdisplay techniqueen_US
dc.subjectCL-repeaten_US
dc.subject玉米zh_TW
dc.subjectB染色體zh_TW
dc.subject顯示技術zh_TW
dc.subjectCL-repeatzh_TW
dc.titleEstablishment and application of CL-repeat display markers in maize B chromosomeen_US
dc.title玉米B染色體CL-repeat顯示標誌之建立與應用zh_TW
dc.typeThesis and Dissertationen_US
dc.date.paperformatopenaccess2017-03-17zh_TW
dc.date.openaccess10000-01-01-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeThesis and Dissertation-
item.cerifentitytypePublications-
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item.languageiso639-1zh_TW-
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