Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/89515
標題: 台灣細本葡萄(Vitisthunbergii Sieb.& Zucc.)花序、結實習性及果實有效成分之研究
Studies on grape inflorescence, fruiting habit, berry character and activity component in fruit of Vitis thunbergii Sieb. & Zucc. in Taiwan
作者: Jiun-Yi Lin
呂俊逸
關鍵字: Vitis thunbergii Sieb.& Zucc.;activity component;berry character;細本葡萄;農藝性狀;有效成分
引用: 牛立新、賀普超。1996。我國野生葡萄屬植物系統分類研究。園藝學報 23 (3):209-212。 甘偉松。1980。台灣藥用植物誌(3)。國立中國醫藥研究所。pp.497。台北。 呂遠平、姚開、李慶。2003。植物單寧降解俊的研究。食品科學24(3):50-53。 向陽、張彤、張?、馬龍。2003。高效液相色譜法測定葡萄皮和葡萄籽中白藜蘆醇的含量。衛生研究32(5):490-492。 沈育杰、趙淑蘭、楊義明、李曉紅、宋潤剛、路文鵬。2006。我國山葡萄種質資源研究與利用現狀。特產研究 3:53-55。 余興、高麗萍、夏濤、胡潁蕙、檀花蓉。2007。葡萄果實生長發育過程中白藜蘆醇及其糖苷的HPLC測定。武漢植物學研究 25(1):84-88。 邱年永、張光雄。1995。原色台灣藥用植物圖鑑(4)。南天書局。pp.147-148。台北。 林俊良、梁文俐、楊玲玲、顏焜熒。1993。山葡萄之成分研究(1)大本山葡萄。鄭氏藥學基金會研究彙刊 1:47-51。 林義恭、劉新裕、賴瑞聲、胡敏夫、高瑞隆、徐源田。2002。台灣原生細本山葡萄的選種與栽培(I)細本山葡萄的外表形態變異。中華農業研究 51(2):24-31。 林義恭。2005。台灣農家要覽增修版定三版。行政院農業委員會。特用作物pp179-182。 苑可武、孟憲惠、徐文豪。1997。銀杏葉中黃酮含量的季節性變化。中草藥 28(4):211-212。 高年法、姜麗、張健、張軍。2005。HPLC法測定葡萄酒中白藜蘆醇的基礎性研究。釀酒 32(1):75-77。 夏開元、戎衛華。2002。葡萄中的功效成分-白藜蘆醇、白藜蘆醇苷和原花青素。食品科學 23(8):356-359。 許再文。1999。台灣產葡萄科植物的分類研究。國立成功大學生物學研究所碩士論文。台南。 陳雷、韓雅珊。1999。葡萄不同品種和組織白藜蘆醇含量的差異。園藝學報 26(2):118-119。 陳迪偉、余建美、胡惠瑜、陳文彥。2001。蛇葡萄之研究及應用。台灣省菸酒公賣局煙類試驗所研究工作年報 pp.25-30。 陳鴻章。2005。國產水果制酒之潛力與機會。農業世界雜誌 262:22-31。 陳韋睿。2007。台灣細本葡萄(Vitis thunbergii Sieb. & Zucc.)莖葉組織解剖特徵之種內變異性。國立中興大學農藝學系碩士論文。台中。 賀普超。1999。葡萄學。中國農業出版社。 管玉民、王健、尤慧蓮、林曉。2000。氣候、季節、樹齡對銀杏葉總黃酮 含量的影響。中成藥 22(5):368-370。 張福平。2004。奧嬰地區野生葡萄植物資源及其開發利用。中國野生植物資源 23(3):11-13。 張立平、林伯年、沈德緒、董繼新。1997。葡萄屬植物核醣體基因的RFLP分析。園藝學報 24(4):385-387。 張薇、胡章勇、王輝憲、熊興耀、李霞。2006。葡萄籽中單寧的提取。作物科學 3:253-255。 劉闖萍、王軍、沈育杰。2007。山葡萄資源性狀評價。東北林業大學學報 35(4):79-81。 蔡佳宏、王子慶、吳明昌。2001。細葉山葡萄及香菇草抗致突變性之研究。科學農業 49(7,8):186-191. 蔡國良。2004。台灣細本山葡萄(Vitis thunbergii Sieb. et Zucc.)之遺傳多樣性。國立中興大學農藝學系碩士論文。台中。 錢大瑋、鞠建明、朱玲英、段金廒、王宇環、張紹君、郭巧生。2002。不同樹齡銀杏葉在不同季節中總黃酮和總內酯的含量變化。中草藥 33(11):1025-1027。 謝文聰、譚思濰、陳介甫、蔡輝彥。1998。山葡萄粗抽取物及其活性成分之鎮痛抗炎作用研究。中國醫藥學院雜誌 7(3):81-87。 應紹舜。1995。台灣高等植物彩色圖誌。第五卷。pp. 571。台北。 顏裕齊。2008台灣細本葡萄(Vitis thunbergii Sieb. & Zucc.)收集系有效成份變異性之研究。國立中興大學農藝學系碩士論文。台中。 蕭書佩。2005。細本葡萄(Vitis thunbergii Sieb. & Zucc.)雌蕊的發育及cytokinins對雄花性別轉換效果之研究。國立中興大學農藝學系碩士論文。台中。 Asou, H., K. koshizuka, T. Kyo, N. Kakata, N. Kamada and H. P. Koeffier. 2002. Resveratrol, a natural product derived from grapes, is a new inducer of differentiation in human myeloid leukemias. Int. J. Hematol. 75(5):528-533. Aviram, M. and B. Fuhrman. 2002. Wine flavonoids protect against LDL oxidation and atherosclerosis. Ann. N. Y. Acad. Sci. 957:146-161. Bagchi, D., A. Garg, R. L.Krohn, M. Bagchi, M. X. Tran, S. J.Stohs, 1997. Oxygen free radical scavenging abilities of vitamins C and E, and a grape seed proanthocyanidin extract in vitro. Res. Comm. Mol. Path. Pharmacol. 95: 179-189. Bagchi, D., M. Bagchi, S. J. Stohs, D. K. Das, S. D. Ray, C. A. Kuszynski, S. S. Joshi, and H. G. Pruess. 2000. Free radicals and grape seed proanthocyanidin extract: importance in human health and disease prevention. Toxicology 148:187-197. Belguendouz, L., L. Fremont, and A. Linard. 1997. Resveratrol inhibits metal ion-dependent and independent peroxidation of porcine low-density lipoproteins. Biochem. Pharmacol. 53:1347-1355. Bertelli, A. A. E., L. Giovannini, D.Giannessi, M. Migliori, W. Bernini. M. Fregoni and A. Bertelli. 1995. Antiplatelet activity of synthetic and natural resvertrol in red wine. Int. J. Tiss. Reac. 17(1):1-3. Burda, S. and W. Oleszek. 2001. Antioxidant and antiradical activities of flavonoids. J. Agric. Food Chem. 49:2774-2779. Chen, R. S., P. L. Wu, and R. Y. Y. Chiou. 2002. Peanut roots as a source of resveratrol. J. Agric. Food Chem. 50:1665-1667. Chung, I. M., M. R. Park, J. C. Chun, and S. J. Yun. 2003. Resveratrol accumulation and resveratrol synthase gene expression in response to abiotic stresses and hormones in peanut plants. Plant Sci. 164:103-109. Crippen, D. D. and J. C. Morrison. 1986. The effects of sun exposure on the phenolic content of Cabernet Sauvignon berries during development. Am. J. Enol. Vitic. 37:243-247. Deschner, E. E., J. Ruperto, G. Wong, and H. L. Newmark. 1991. Quercetin and rutin as inhibitors of azoxymethanol-induced colonic neoplasia. Carcinogenesis 12(7):1193-1196. Ding, C., E. Chen, and R. C. Lindsay. 2007. Natural accumulation of terpene trilactones in Ginkgo biloba leaves: variations by gender, age and season. Eur. Food Res. Technol. 224:615-621. Dubber, M. J. and I. Kanfer. 2004. High-performance liquid chromatographic determination of selected flavonols in Ginkgo biloba solid oral dosage forms. J. Pharm. Pharmaceut. Sci. 7(3):303-309. Frankel, E., A. Waterhouse, and J. Kinsella. 1993. Inhibition of human LDL oxidation by resveratrol. Lancet 341:1103-1104. Frankel, E. N., A. L. Waterhouse, and P. L. Teissedre. 1995. Principal phenolic phytochemicals in selected California wines and their antioxidant activity in inhibiting oxidation of human low-density lipoproteins. J. Agric. Food Chem. 43:890-894. Frankel, E. N., C. A. Bosanek, A. S. Meyer, K. Silliman, and L. L. Kirk. 1998. Commercial grape juices inhibit the in Vitro oxidation of human low-density lipoproteins. J. Agric. Food Chem. 46:834-838. Goto-Yamamoto, N., R. Mochioka, L. Bonian, K. Hashizume, N. Umeda, and S. Horiuchi. 1998. RFLP and RAPD analysis of wild and cultivated grapes (Vitis spp.). J. Japan. Soc. Hort. Sci. 67(4):483-490. Goto-Yamamoto, N. 2000. Phenetic clustering of grapes (Vitis spp.) by AFLP analysis. Breed. Sci. 50:53-57. Hertog, M. G. L., E. J. M. Feskens, P. C. H. Hollman, M. B. Katan, and D. Kromhout. 1993. Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen elderly study. Lancet 342:1007-1011. Hsieh, C. F., T. C. Huang, Z. Y. Li, H. C. Lo, H. Ohashi, C. F. Shen, J. C. Wang, K. C. Yang, C. M. Hu, and H. Y. Yang. 1993. Flora of Taiwan volume three, second edition. pp.709-710. Taipei. Huang, Y. L., W. J. Tsai, C. C. Shen, and C. C. Chen. 2005. Resveratrol derivatives from the roots of Vitis thunbergii. J. Nat. Prod. 68:217-220. Jang, M., L. Cai, G. O. Udean, K. V. Slowing, C. F. Thomas, C. W. W. Beecher, H. H. S. Fong, N. R. Farnsworth, A. D. Kinghorn, R. G. Mehta, R. C. Moon and J. M. Pezzuto. 1997. Cancer chemopreventive activity of resveratrol, a natural product derived from grapes. Science 275:218-220. Jang, M. and J. M. Pezzuto. 1999. Cancer chemopreventive activity of resveratrol. Drugs Exptl. Clin. Res. 25(2):65-77. Knekt, P., J. Kumpulainen, R. Jarvinen, H. Rissanen, M. Heliovaara, A. Reunnanen, and T. Hakulinen. 2002. Flavonoid intake and risk of chronic diseases. Am. J. Clin. Nutr. 76:560-568. Kondo K., R. Uchida, S. Tokutake and T. Maitani. 1999. Polymeric grape-seed procyanidins, but not monomeric catechins and oligomeric procyanidins, impair degranulation and membrane ruffling in RBL-2H3 cells, Bioorg. Med. Chem. 14 641–649. Lee, J. J., K. M. Crosby, L. M. Pike, K. S. Yoo, and D. I. Leskovar. 2005. Impact of genetic and environmental variation on development of flavonoids and carotenoids in pepper (Capsicum spp.). Sci. Hortic. 106:341-352. Li, H. F., S. A. Chen and S. N. Wu. 2000. Evidence for the stimulatory effect of resveratrol on Ca2+-activated K+ current in vascular endothelial cells. Cardiovasc Res 45:1035-1045. Lin, J. K. and S. H. Tsai. 1999. Chemoprevention of cancer and cardiovascular disease by resveratrol. Proc. Natl. Sci. Conc. 23:99-106. Liu, L. and A. Castonguay. 1991. Inhibition of the metabolism and genotoxicity of 4- (methylnitrosamino)-1- (3-pyridyl)-1-butanone (NNK) in rat hepatocytes by (+)-catechin. Carcinogenesis 12(7):1203-1208. Liu, J. C., F. L. Hsu, J. C. Tsai, P. Chan, J. Y. H. Liu, G. N. Thomas, B. Tomlinson, M. Y. Lo and J. Y. Lin. 2003. Antihypertensive effects of tannins isolated from traditional Chinese herbs as non-sprcific inhibitors of angiotensin converting enzyme. Life Sci. 73:1543-1555. Lobstein, A., L. Rietsch-Jako, M. Haag-Berrurier, and R. Anton. 1991. Seasonal variation of the flavonoid content from Ginkgo biloba leaves. Planta Med. 57:430-433. Lu, Y. and L. Y. Foo. 1999. The polyphenol constituents of grape pomace. Food Chem. 65:1-8. Luo, S. L., P. C. He, X. Q. Zheng, and P. Zhou. 2001. Genetic diversity in wild grapes native to China based on randomly amplified polymorphic DNA (RAPD) analysis. Acta Botanica Sinica 43(2):158-163. Merillon, J. M., B. Fauconneau, P. W. Teguo, L. Barrier, J. Vercauteren, and F. Huguet. 1997. Antioxidant activity of the stilbene astringin, newly extracted from Vitis vinifera cell cultures. Clin. Chem. 43(6):1092-1093. Pedrielli, P., G. F. Pedulli, and L. H. Skibsted. 2001. Antioxidant mechanism of flavonoids solvent effect on rate constant for chain-breaking reaction of quercetin and epicatechin in antioxidation of methyl linoleate. J. Agric. Food Chem. 49:3034-3040. Pekkarinen, S. S., I. M. Heinonen, and A. I. Hopia. 1999. Flavonoids quercetin, myricetin, kaemferol and (+)-catechin as antioxidant in methyl linoleate. J. Sci. Food Agric. 79:499-506. Pietta, P. G. 2000. Flavonoids as antioxidants. J. Nat. Prod. 63:1035-1042. Renaud, S. and M. Lorgeril. 1992. Wine, alcohol, platelets, and the French paradox for coronary heart disease. Lancet 339:1523-1526. Rice-Evans, C. A., N. J. Miller, and G. Paganga. 1996. Structure-antioxidant activity relationships of flavonoids and phenolic acid. Free Radic. Biol. Med. 20(7):933-956. Sanders, T. H., R. W. McMichael, and K. W. Hendrix. 2000. Occurrence of resveratrol in edible peanuts. J. Agric. Food Chem. 48:1243-1246. Santos-Buelga, C. and A. Scalbert. 2000. Proanthocyanidins and tannin-like compounds ature, occurrence, dietary intake and effects on nutrition and health. J. Sci. Food Agric. 80:1094-1117. Santos-Buelga, C., E. M. Francia-Aricha, and M. T. Escribano-Bailon. 1995. Comparative flavan-3-ol composition of seeds from different grape varieties. Food Chem. 53:197-201. Shyur, L. F., J. H. Tsung, J. H. Chen, C. Y. Chiu, and C. P. Lo. 2005. Antioxidant properties of extracts from medicinal plants popularly used in Taiwan. Int. J. Appl. Sci. Eng. 3(3):195-202. Soleas, G. J., L. Grass, P. D. Josephy, D. M. Goldberg, and E. P. Diamandis. 2006. A comparison of the anticarcinogenic properties of four red wine polyphenols. Clin. Biochem. 39:492-497. Subbaramaiah, K., W. J. Chung, P. Michaluart, N. Telang, T. Tanabe, H. Inoue, M. Jang, J.M. Pezzuto and A.J. Dannenberg. 1998. Resveratrol inhibits cyclooxygenase-2 transcription and activity in phorbol ester-treated human mammary epithelial cells, J. Biol. Chem. 273: 21875-21882. van Beek, T. A. and G. P. Lelyveld. 1992. Concentration of ginkgolides and bilobalide in Ginkgo biloba leaves in relation to the time of year. Planta Med. 58:413-416. Wang, H., G. Gao, and R. L. Prior. 1997. Oxygen radical absorbing capacity of anthocyanins. J. Agric. Food Chem. 45:304-309. Wang, K. H., Y. H. Lai, J. C. Chang, T. F. Ko, S. L. Shyu, and R. Y. Y. Chiou. 2005. Germination of peanut kernels to enchance resveratrol biosynthesis and prepare sprouts as a functional vegetable. J. Agric. Food Chem. 53:242-246. Wang, Z., Y. Huang, J. Zou, K. Cao, Y. Xu, and J. M. Wu. 2002. Effects of red wine and wine polyphenol resveratrol on platelet aggregation in vivo and in vitro. Int. J. Mol. Med. 9:77-79. Weber, G., F. Shen, N. Prajda, H. Yang, W. Li, A. Yen, B. Csokay, E. Olah, and K. Y. Look. 1997. Regulation of the signal transduction program by drugs. Adv. Enzyme Regul. 37:35-55. Yilmaz, Y. and R. T. Toledo. 2004. Major flavonoids in grape seeds and skins: antioxidant capacity of catechin, epicatechin, and gallic acid. J. Agric. Food Chem. 52:255-260.
摘要: 
本試驗以80個細本葡萄收集系為試驗材料,探討細本葡萄生育期間花序、結果習性與果實經濟性狀以及收集系果實中白藜蘆醇、白藜蘆醇苷、山柰酚以及類黃酮醣苷等有效成分含量之遺傳變異性,並建立其資料庫作為品種鑑定和育種選拔之參考。並且分析不同組織部位和果實發育階段中有效成分含量之差異,作為栽培利用之參考。本試驗調查細本葡萄4個花器性狀與8果實經濟性狀,結果顯示各性狀在收集系間均有明顯的變異。由各性狀間之相關關係結果顯示果實甜度與果皮呈色有顯著正相關關係,但與果翼數呈顯著負相關。變方分析結果得知,有效成分含量在收集系間均有顯著差異。在不同組織部位有效成分含量變方分析得知,除芸香苷外,均有顯著差異;種子中以白藜蘆醇和白藜蘆醇苷的含量較高,而果皮和果梗中以山柰酚及類黃酮糖苷含量較高。分析各有效成分之相關關係顯示,山柰酚之含量與金絲桃苷及異槲皮苷呈顯著之正相關。探討各有效成分與果實性狀之相關關係得知,白藜蘆醇苷與單果穗重呈顯著正相關,但與金絲桃苷、異槲皮苷及山柰酚呈顯著負相關。山柰酚與黃酮類糖苷含量以綠果期含量較高,白藜蘆醇及白藜蘆醇苷之含量則無明顯差異,可知綠果期果實,即可採收利用。本研究所建立之果實形態特徵資料庫可作為細本葡萄在種原利用、蒐集和品種鑑定上的參考。

80 Vitis thunbergii collections from Taiwan island were used in this study. Quantitative traits variation in inflorescence, berry characters, berry economic characters and amount of active compositions of berry, such as resveratrol, piceid, kaempferol and flavonoids, in reproduction development have been investigated;and the dataset of these quantitative traits have been constructed to conduct cultivars identification and selection breeding. In addition, the amounts of active compositions in different tissues and at different berry developmental stages have been analyzed and compared to guide cultivation and utilization. After investigating 4 flower organs and 8 berry economic characters, each character shows clear variation among collections. Sugar content of berry is positively correlated to color of berry whereas negatively to number of wings according to correlation coefficient analysis of 12 characters. The amounts of active compositions among collections have significant difference according to ANOVA result. Therefore, there is an opportunity to breed collections containing high amounts of active compositions. The amounts of active compositions among different tissues excluding rutin, have significant difference according to ANOVA result. Particularly, the highest amounts of active compositions in the seed are resveratrol and piceid, while in peel and pedicel are kaempferol and flavonoids. The amount of kaempferol is positively correlated to hyperin and isoquercitrin according to correlation coefficient analysis of active compositions. Single berry weight is positively correlated to amount of resveratrol whereas negatively to amount of hyperin, isoquercitrin and kaempferol according to correlation coefficient analysis of active compositions and berry characters. Consequently, the berry of collection contained more amount of resveratrol maybe have less amount of other compositions. Kaempferol and flavonoids are the highest amounts of active compositions in berry, especially at green stage. Furthermore, the amounts of resveratrol and piceid have no difference among different berry developmental stages. Consequently, the berry at green stage can be harvested and utilized. Finally, the berry conformation dataset of V. thunbergii in this study can be applied to cultivation, germplasm collection and cultivars identification.
URI: http://hdl.handle.net/11455/89515
其他識別: U0005-0907201510543000
Rights: 同意授權瀏覽/列印電子全文服務,2015-07-16起公開。
Appears in Collections:農藝學系

Files in This Item:
File SizeFormat Existing users please Login
nchu-97-79531104-1.pdf2.42 MBAdobe PDFThis file is only available in the university internal network    Request a copy
Show full item record
 

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.