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標題: 紫錐菊花藥誘導植株之檢定與利用
Detection and application of anther culture-derived plants of Echinacea purpurea (L.) Moench
作者: Shin-Jie Huang
關鍵字: anther culture;Echinacea purpurea L.;self-incompatibility;ISSR;花藥培養;紫錐菊;自交不親和性;ISSR
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紫錐菊 (Echinacea spp.) 為北美原生草本多年生植物,其中一種紫花紫錐菊 (Echinacea purpurea (L.) Moench) 是目前廣受培育的花卉、藥用植物,在商業生產上主要以種子繁殖為主,由於紫錐菊具有自交不親合性,(self-incompatibility)且為雄蕊先熟型,主要透過昆蟲進行授粉,形成異質結合體 ,個體之間農藝性狀與成分含量差異甚大,在商業生產上不易掌控品質。本研究利用花藥培養誘導產生植株,利用根尖染色體檢查及流式細胞儀檢定其倍數性,植株開花後進行自交以檢定其自交不親合性指數(index self-incompatibility,ISI)。同時利用簡單虛列重複區間(inter simple sequence repeats, ISSR)分子標誌分別檢定親本、誘導株、誘導株自交與雜交後裔,亦進行後裔其他植株性狀比較,以確認花藥培養誘導株是具有自交不親合性的同質雙單倍體。倍數性檢定顯示花藥培養誘導株為二倍體,形態特徵的表現顯示,自交後代的莖葉形狀不完全相同,利用ISSR分子標誌及後裔檢定法檢定親本、誘導株、誘導株自交與雜交後裔證明花藥培養誘導株並非雙單倍體,以自交不親合性指數評估,花藥誘導株有2棵為具完全自交不親合性、2棵為具有部分自交不親合性及4棵為具有自交親和性。另外在第二批花藥培養產生誘導株以ISSR檢測顯示具有極大潛力為同質體,並且同時檢測其自交不親和性,其中有四株具有自交不親和性。未來將進一步調查其農藝性狀,以期能獲得據有高度自交不親和性的雙單倍體植株。

Echinacea (Echinacea spp.), commonly known as coneflower, is a North American native genus of perennials plants. One species, E. purpurea or the purple coneflower, is a very popular ornamental and medicinal plant, and has been subject to intensive breeding efforts. Commercial production of Echinacea mainly propagated by seed, however, Echinacea with the characteristics of self-incompatibility and protandrous, mainly through insects as pollinator. For cross-pollinated plants, it might form a heterogeneous group, propagated by seed will lead to agronomic traits and composition variation, hard to control quality.
In the present study, anther-derived plants of E. purpurea were evaluated by chromosome number examination of root-tip, flow cytometry, morphological character discrimination and inter simple sequence repeat (ISSR) markers. Results of chromosome number and chromosomal DNA content confirmed that all of anther-derived plantlets were diploid. Morphological traits showed that they were not identical in selfing progeny. ISSR analysis of parents, anther-derived plant, self and cross-pollinated offspring demonstrate that first lot of anther-derived plantlets were not a homogeneous doubled haploid. However, some of second lot of anther-derived plantlets could be potentially homogeneous doubled haploid by ISSR analysis. To evaluate the degree of self-incompatibility by index of self-incompatibility among first lot of selfing and crossing materials, results indicated that two plantlets and four plantlets belongs to completely self-incompatible and mostly self-incompatible, respectively.
其他識別: U0005-2811201416195976
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