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標題: 聖約翰草有效成分於不同栽培條件下之含量 與其微波輔助萃取方法之研究
Study on the contents of active ingredients in St..John's wort(Hypericum perforatum L .)under .different cultivation conditions and their .extraction with microwave-assisted method
作者: Yi-Chin Chou
關鍵字: no;無
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聖約翰草具有多種有效成分,為治療憂鬱症及其他疾病的重要藥用植物來源。為了解聖約翰草累積有效成分之最佳條件,以3 種等級之溫度、光照及營養液栽培聖約翰草,利用高效液相色層分析儀測定植株2 個月大時hyperforin、hypericin、melatonin、quercetin、rutin 等成分之含量變化,發現hyperforin、melatonin、rutin 含量受到溫度與光照顯著之影響,然而施用營養液的處理則未有明顯之影響,推測與營養液之施用濃度和種類有關。根據反應曲面法,可分別找出往後栽培聖約翰草最適化試驗條件的方向。
微波輔助萃取法的試驗中發現在550 W、60 分鐘;300 W、80 分鐘;750 W、40分鐘;1000 W、10 分鐘100 W、40 分鐘處理下可分別從聖約翰草葉片中得到高含量之hyperforin、hypericin、melatonin、quercetin、rutin。以上述方法分析莖葉花中活性成分之含量,發現物質多累積於葉、花,其中又以花朵為主。在溶劑萃取效果的比較中,則以甲醇、乙醇之萃取效果較佳。此外,從開花中聖約翰草的莖葉中可分離出3 株內生真菌,分別為S-3-A、L-3-B及L-3-C,以培養基PDB、YMB 作液態培養,約50 天後發現其可能具產生hyperforin及 melatonin 等二次代謝物之潛力,顯示此藥草可能利用此種共生模式來增加特定活性物質的累積,甚至作為抑菌、防禦病原體之用途。

St. John's wort has been a vary important medicinal plant to alleviate depression and other disorders owing to its abundance of several active ingredients. In order to investigate the optimal conditions for accumulation of active ingredients of St. John's wort, the variation of
hyperforin, hypericin, melatonin, quercetin and rutin contents of two month-old plants cultivated under 3 levels of temperature, light and nutrient solution have been analysed by HPLC. It reveals that the contents of hyperforin, melatonin and rutin of St. John's wort has been influenced significantly by temperature and light, whereas with no signifacant effects by nutrient solution. It should be related with applied levels and kinds of nutrient. Based on Response Surface Methodology, the best-fit future direction of optimal conditions for cultivation of St. John's wort can be derived, respectively.
In the experiments of microwave-assisted extraction, the highest amounts of hyperforin ,hypericin, melatonin, quercetin, and rutin can be obtained from leaves of St. John's wort under 550 W×60 min ; 300 W× 80 min ; 750 W×40 min ; 1000W×10 min and 100 W×40 min, respectively. The amounts of active ingredients of stems, leaves and flowers of St. John's wort have been analysed by afore-mentioned method. Besides rutin, the other active compounds occur mainly in leaf and flower tissues of St. John's wort, especially in flowers. Among different
solvents, methanol and ethanol have shown to be better with microwave-assisted extraction of active ingredients of St. John's wort.
In addition, three endophytic fungi designated as S-3-A, L-3-B and L-3-C have been isolated from stem and leaf parts of St. John's wort in bloom. The isolated endophytes were grown in liquid culture with PDB and YMB media for about 50 days. It has been found that these endophytes might posses potential to produce hyperforin and melatonin and other secondary metabolites. It exhibits that this medicinal herb can use this proposed mode of co-existence with endophytes to increase accumulation of certain active ingrediants for
antibacterial and pathogen defense purposes.
其他識別: U0005-0207201511195200
Rights: 同意授權瀏覽/列印電子全文服務,2015-07-16起公開。
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