Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/89552
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dc.contributor胡澤寬zh_TW
dc.contributor.authorYa-Jyun Shenen_US
dc.contributor.author沈雅鈞zh_TW
dc.contributor.other農藝學系所zh_TW
dc.date2015zh_TW
dc.date.accessioned2015-12-07T08:45:38Z-
dc.identifierU0005-1701201515525200zh_TW
dc.identifier.citation羅聖賢、林正宏、王俊達。2003。以RAPDs分子標誌鑑定臺灣原生蝴蝶蘭親緣關係之研究。台東區農業改良場研究彙報14: 19-38。 羅聖賢、林正宏。2003。臺灣原生蝴蝶蘭在形態和遺傳上特性的分類歸屬。台東區農業改良場研究彙報14:1-18。 Agarwal, M., N. Shrivastava and H. Padh. 2008. Advance in molecular marker techniques and their applications in plant sciences. Plant Cell rep. 27:617-631. Aneja, B., R. Y. Neelam, C. Veena and R. C. Yadav. 2012. Sequence-related amplified polymorphism (SRAP) molecular marker system and its applications in crop improvement. Mol. Breed. 30:1635-1648. Arends, J. C. 1970. Cytological observation on genome homology in eight interspecific hybrids of Phalaenopsis. Genetica 41:88-100. Bray, J. R. and J. T. Curtis. 1957. An ordination of upland forest communities of southern Wisconsin. Ecol. Monogr. 27:325-349. Cai, X., Z. Feng, X. Zhang, W. Xu, B. Hou and X. Ding. 2011. Genetic diversity and population structure of an endangered Orchid (Dendrobium loddigesii Rolfe) from China revealed by SRAP markers. Sci. Hortic. 129:877-881. Chen, W. H., Y. L. Kao, C. Y. Tang, C. C. Tsai and T. Y. Lin. 2013. Estimating nuclear DNA content within 50 species of the genus Phalaenopsis Blume (Orchidaceae). Sci. Hortic. 161:70-75. Chiou W. L. and T. Y. Chiang. 2013. Plant systematics. SMC Publishing, Taipai,Taiwan. 274-296 pp. Chritenson, E. A. 2001. Phalaenopsis a monograph. Timber Press, Portland, Oregon. USA. Chuang, H. T., T. M. Shen, Y. F. Yen, C. P. Liu and S. H. Cheng. 2004. Phylogenetic Analysis of Five Phalaenopsis Species Native to Taiwan or the Philippines by Using RAPD and ISSR Molecular Markers. J. Chinese Soc. Hort. Sci. 50: 175-186. Dice, L. R. 1945. Measures of the amount of ecologic association between species. Eecology. 26:297-302. Ding, G., D. Zhang, X. Ding, Q. Zhou, W. Zhang and X. Li. 2008. Genetic variation and conservation of the endangered Chinese endemic herb Dendrobium officinale based on SRAP analysis. Plant Syst. Evol. 276:149-156. Dressler, R. L. 1993. Phylogeny and classification of the orchid family. Timber Press,Portland Oregon. 206-207pp. Feng, S., H. Zhao, J. J. Lu, J. J. Liu, B. Shen and H. Z. Wang. 2013. Preliminary genetic linkage maps of Chinese herb Dendrobium nobile and D. moniliforme. J. Genetics. 92:205-212. Fu, Y. M., W. H. Chen, W. T. Tsai, Y. S. Lin, M. S. Chyou and Y. H. Chen. 1997. Phylogenetic studies of taxonomy and evolution among wild species of Phalaenopsis by random amplified polymorphic DNA marker. Rept. Taiwan Sugar Res. Inst. 157:27-42. Goh, M. W. K., P. P. Kumar, S. H. Lim and H. T. W. Tan. 2005. Random amplified polymorphic DNA analysis of the moth orchids, Phalaenopsis (Epidendroideae: Orchidaceae). Euphytica 141:11-22. Huang, T. 2014. Guide to orchid in class Ⅱ. TOGA, Tainan, Taiwan. 204-268pp. Ibrahim, R. I. H. 2011. A modified CTAB protocol for DNA extraction from young flower petals of some medicinal plant species. Geneconserve. 10:165-182. Jian, B., H. Xu, S. T. Zhou and H. Z. Wang. 2012. Genetic diversity of 47 Cymbidium ensifolium varirties assessed by SRAP markers. J. Plant Genet. Resour. 13:376-380. Jiang, C., Y. Wang, Y. Sun. 2007. Application advance of molecular marker techniques of SSR and ISSR. Chin. Tob. Sci. 28:1-5. Kao, Y. Y., S. B. Chang, T. Y. Lin, C. H. Hsien, Y. H. Chen, W. H. Chen and C. C. Chen. 2001. Differential accumulation of heterochromatin as a cause for karyotype variation in Phalaenopsis Orchids. Ann. Bot. 87:387-395. Kumar, P., V. K. Gupta, A. K. Misra, D. R. Modi and B. K. Pandey. 2009. Potential of molecular markers in plant biotechnology. Plant Omics. 2:141-162. Li, G. and C. F. Quiros. 2001. Sequence-related amplified polymorphism (SRAP) , a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor. Appl. Genet. 103:455-461. Lin, S., H. C. Lee, W. H. Chen, C. C. Chen, Y. Y. Kao, Y. M. Fu, Y. H. Chen and T. Y. Lin. 2001. Nuclear DNA contents of Phalaenopsis sp. and Doritis pulcherrima. J. Amer. Soc. Hort. Sci. 126:195-199. Lin, X. C, Y. F. Lou, Y. Z. Zhang, X. L. Yuan, J. C. He and W. Fang. 2011. Identification of genetic diversity among cultivars of Phyllostachys violascens using ISSR, SRAP and AFLP markers. Bot. Rev. 77:223-232. Lin, C. C., Y. H. Chen, W. H. Chen, C. C. Chen and Y. Y. Kao. 2005. Genomic organization and relationships of Phalaenopsis orchids inferred from genomic in situ hybridization. Bot. Bull. Acad. Sin. 46:339-345. Liu, C. X., Q. Y. He and W. W. Jin. 2010. The paring, synapsis and recombination of meiosis in plant. Hereditas (Bejing) 32: 1223-1231. Liu, L. W., L. P. Zhao, Y. Q. Gong, M. X. Wang, L. M. Chen, J. L. Yang, Y. Wang, F. M. Yu and L. Z. Wang. 2008. DNA fingerprinting and genetic diversity analysis of late-bolting radish cultivars with RAPD, ISSR and SRAP markers. Sci. Hortic. 116:240-247. Liu, Z. J., S. C. Chen and Z. Z. Ru. 2005. Phalaenopsis malipoensis, a new species of Orchidaceae from China. Acta Botanica Yunnanica 27:37-38. Lu, J. J., S. Wang, H. Y. Zhao, J. J. Liu and H. Z. Wang. 2012. Genetic linkage map of EST-SSR and SRAP markers in the endangered Chinese endemic herb Dendrobium (Orchidaceae). Genet. Mol. Res. 11:4654-4667. Massaki, C. 2002 Chiba Massaki Phalaenopsis Species Publishing Society, Saitama-kenn, Japan. Niknejad, A., M. A. Kadir, S. B. Kadzimin, N. A. P. Abdullah and K. Sorkheh. 2009. Molecular characterization and phylogenetic relationships among and within species of Phalaenopsis (Epidendroideae: Orchidaceae) based on RAPD analysis. Afr. J. Biotechnol. 8:5225-5240. Pan, Z. J., C. C. Cheng, W. C. Tsai, M. C. Chung, W. H. Chen, J. M. Hu and H. H. Chen. 2011. The duplicated B-class MADS-box genes display dualistic characters in orchid floral organ identity and growth. Plant Cell Physiol. 52:1515-1531. Park, P. H., Y. J. Prak, M. S. Kim, Y. R. Lee., P. M. Park, D. S. Lee and B. W. Yae. 2013. Analysis of generc diversity and identification of domestic bred Phalaenopsis varieties using SRAP and SSR marker. Kor. J. Hort. Technol. 31:337-343. Patwardhan, A., S. Ray and A. Roy. 2014. Molecular markers in phylogenetic studies-A review. J. Phylogen Evol. Biol. 2:131-139. Pejic, I., P. Ajmone-Marsan, M. Morgante, V. Kozumplick, P. Castiglioni, G. Taramino and M. Motto. 1998. Comparative analysis of genetic similarity among maize inbred lines detected by RFLPs, RAPDs, SSRs, and AFLPs. Theor. Appl. Genet. 97: 1248-1255. Pinheiro, L. R., A. R. C. Rabbani, A. V. C. da Silva, A. da Silva Lédo and L. E. C. Diniz. 2012. Genetic diversity and population structure in the Brazilian Cattleya labiate (Orchidaceae) using RAPD and ISSR markers. Plant Syst. Evol. 298:1815-1825. Pridgeon, A. M., P. J. Cribb, M. W. Chase and F. N. Rasmussen. 2014. Genera Orchidacearum Volume 6 Epidendroideae (Part 3). Oxford University Press. 233-241pp. Prevost, A. and M. J. Wilkinson. 1999. A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theor. Appl. Genet. 98:107-112. Reddy, M. P., N. Sarla and E. A. Siddiq. 2002. Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Eupytica 128:9-17. Roldán-Ruiz, I., J. Dendauw, E. Van Bockstaele, A. Depicker and M. De Loose. 2000. AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.). Mol. Breed. 6:125-134. Rindyastuti, R. 2013. Bioinformatics application in search of phylogenetic relationship on eleven species of Phalaenopsis Blume (Orchidaceae) based on gen internal transcribed spacer (ITS1 and ITS2), 5.8 DNA Ribosomal. Proceeding ICGRC. Robarts, D. W. H. and A. D. Wolfe. 2014. Sequence-related amplified polymorphism (SRAP) markers: a potential resource for studies in plant molecular biology. Appl. Plant Sci. 2:1400017. doi:10.3732/apps.1400017. Schwarzacher, T. 2003. Meiosis, recombination and chromosomes: a review of gene isolation and fluorescent in situ hybridization data in plants. J. Exp. Bot. 54:11-23. Semagn, K., Å. Bjørnstad and M. N. Ndjiondjop. 2006. An overview of molecular marker methods for plants. Afr. J. Biotechnol. 5: 2540-2568. Shao, Q. S., Q. S. Guo, Y. M. Deng and H. P. Guo.2010. A comparative analysis of genetic diversity in medicinal Chrysanthemum morifolium based on morphology, ISSR and SRAP markers. Biochem. Syst. Ecol. 38:1160-1169. Sweet, H. R. 1980. The Genus Phalaenopsis. Orchid Digest Crop., Pomona, USA. Stewart, S. and M. Griffiths. 1995. Manual of Orchids Timber Press, Protland Oregon. USA. 278-282pp. Taywiya, P. 2010. Analysis of Genetic Relationship and Marker of Genus Phalaenopsis and Hybrids by Molecular Techniques. PhD. Dissertation Chiang Mai Univ., Thailand. Tsai, C. C., S. C. Hung and C. H. Chou. 2009. Phylogenetics, biogeography, and evolutionary trends of the Phalaenopsis sumatrana complex inferred from nuclear and chloroplast DNA. Biochem. Syst. Ecol. 37:633-639. Tsai, C. C., C. R. Sheue, C. H. Chen and C. H. Chou. 2010a. Phylogenetics and biogeography of the Phalaenopsis violacea (Orchidaceae) species complex based on nuclear and plastid DNA. J. Plant Biol. 53:453-460. Tsai, C. C., Y. C. Chiang, S. C. Huang, C. H. Chen and C. H. Chou. 2010b. Molecular phylogeny of Phalaenopsis Blume (Orchidaceae) on the basis of plastid and nuclear DNA. Plant Syst. Evol. 288:77-98. Wang, L. L., L. P. Zhao, Y. Q. Gong, M. X. Wang, L. M. Chen, J. L. Yang, Y. Wang, F. M. Yu and L. Z. Wang. 2008. DNA fingerprinting and genetic diversity analysis of late-bolting radish cultivars with RAPD, ISSR and SRAP markers. Sci. Hortic. 16: 240-247. Wang, H. Z., Z. X. Wu, J. J. Lu, N. N. Shi, Y. Zhao, Z. T. Zhang and J. J. Liu. 2009. Molecular diversity and relationships among Cymbidium goeringii cultivars based on inter-simple sequence repeat (ISSR) markers. Genetica 136:391-399. Wang, Z., J. E. Wang, X. M. Wang, H. W. Gao, N. I. Dzyubenko and V. F. Chapurin. 2012. Assessment of genetic diversity in Galega officinalis L. using ISSR and SRAP markers. Genet. Resour. Crop Evol. 59:865-873. Wu, Z. G., X. X. Li, X. C. Lin, W. Jiang, Z. M. Tao, N. Mantri, C. Y. Fan and X. Q. Bao. 2014. Genetic diversity analysis of yams (Dioscorea spp.) cultivated in China using ISSR and SRAP markers. Genet. Resour. Crop Evol. 61:639-650. Xie, Q., N. S. Miao, X. M. Song, D. H. Huang, B. G. Li and P. Zhao. 2010. Genetic diversity analysis of Phalaenopsis by ISSR marker. Acta. Bot. Boreal. -Occident Sin. 30:1331-1336. Xin, Z., J. P. Velten, M. J. Oliver and J. J. Burke. 2003. High-throughput DNA extraction method suitable for PCR. BioTechniques 34:820-826. Xue, D., S. Feng, H. Zhao, H. Jiang, B. Shen, N. Shi, J. Lu, J. Liu and H. Wang. 2010. The linkage maps of Dendrobium species based on RAPD and SRAP markers. J. Genet. Genomics. 37:197-204. Yukawa, T., K. Kita, T. Handa, T. Hidayat and M. Ito. 2005. Molecular phylogenetics of Phalaenopsis (Orchidaceae) and allied genera: re-evaluation of generic concepts. Acta. Phytotax. Geobot. 56:141-161. Zietkiewicz, E., A. Rafalski and D. Labuda. 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20:176-183.zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/89552-
dc.description.abstract本研究為探討蝴蝶蘭屬物種之遺傳歧異度,利用形態、ISSR及SRAP等資訊作分群分類之分析,以形態、ISSR、SRAP或合併兩種分子標誌之分析結果對蝴蝶蘭屬遺傳歧異度評估與分群分類可得相近之結果。綜合三種分析方式與合併兩種分子標誌之分析結果可得幾個結論,其中Parishianae亞屬獨立為單獨一個類群有別於其他分類地位之物種,再者Polychilos亞屬內的Polychilos、Fuscatae、Amboinensis以及Zebrinae等四個節常被相互歸為同一類群,顯示亞屬內各節彼此都具有相近的親緣關係,而Phalaenopsis亞屬Phalaenopsis、Stauroglottis節兩者有較相近的遺傳距離,且不與Phalaenopsis亞屬Deliciosa、Esmeralda節歸為同一類群,顯示Phalaenopsis亞屬內各節親緣關係不為相近,最後Aphyllae亞屬常被歸到各類群中,與不同分類地位的物種都有相近的遺傳距離,顯示與各分類地位均有一定程度的親緣關係。由本研究之結果有助於研究蝴蝶蘭屬原生種之遺傳歧異度與親緣關係,並可得知ISSR和SRAP分子標誌應用在蝴蝶蘭遺傳研究之效率,未來將有助於種質保存與遺傳育種方面之利用。zh_TW
dc.description.abstractTo investigate the genetic diversity among genera Phalaenopsis species, morphology characters, ISSR and SRAP marker were performed in this study. The performance of morphology characters, ISSR and SRAP markers, single or in combination for genetic diversity assessment and classification were similar. In summary, samples from the subgenus Parishianae were separate clustered different from other taxonomic status, and samples form the four section Polychilos, Fuscatae, Amboinensis and Zebrinae of subgenus Polychilos were frequently clustered together in, this indicated that the genetic diversity among each section of this subgenus were similar, then samples from section Phalaenopsis and Stauroglottis of subgenus Phalaenopsis often had a similar genetic distance and different from section Deliciosa and Esmeralda of subgenus Phalaenopsis. Finally, samples from subgenus Aphyllae were frequently clustered together with other taxonomic status, showed closely relate. This study showed that both ISSR and SRAP markers could be an efficient tool for study genetic diversity and relationship of genus Phalaenopsis kinship native orchid species, this could contribute germplasm conservation and breeding native orchid species in the future.en_US
dc.description.tableofcontents章 節 中文摘要 i 英文摘要 ii 目 錄 1 表 目 錄 3 圖 目 錄 4 附 錄 5 第一章 緒 論 7 第二章 前人研究 8 一、 蝴蝶蘭屬植物分類學地位、地理分布及形態概述 8 二、 分子標誌在遺傳研究之應用 8 (一) 簡單序列區間重複(inter-simple sequence repeat, ISSR) 9 (二) 相關序列增幅多型性(sequence-related amplified polymorphism, SRAP) 10 (三) 不同分子標誌之遺傳歧異度分析能力 10 三、 蝴蝶蘭屬分群分類之研究 11 (一) 形態學研究 11 (二) 細胞遺傳學與DNA含量 12 (三) 分子標誌 12 第三章 材料方法 14 一、 試驗材料 14 二、 形態特徵調查 14 (一) 葉部特徵(characteristics of leaf) 15 (二) 花梗特徵(characteristics of inflorescence) 15 (三) 花部特徵(characteristics of flower) 15 (四) 上萼瓣特徵(characteristics of dorsal sepal) 15 (五) 下萼瓣特徵(characteristics of lateral sepal) 16 (六) 翼瓣特徵(characteristics of petal) 16 (七) 唇瓣特徵(characteristics of lip) 17 (八) 中央裂片特徵(characteristics of apical lobe) 17 (九) 側裂片特徵(characteristics of lateral lobe) 18 三、 DNA萃取作業 18 四、 分子標誌之反應條件 19 五、 膠體分析 19 六、 統計分析 20 第四章 結 果 21 一、 形態特徵調查分析結果 21 二、 DNA萃取作業 22 三、 ISSR分子標誌分析結果 22 四、 SRAP分子標誌分析結果 23 五、 合併ISSR與SRAP分子標誌資料之分析結果 24 第五章 討 論 25 一、 DNA萃取作業 25 二、 形態特徵之分析 26 三、 比較兩種分子標誌之分析能力 27 四、 綜合形態特徵、地理分布、DNA含量及分子證據結果 28 第六章 結 論 30 第七章 參考文獻 31zh_TW
dc.language.isozh_TWzh_TW
dc.rights同意授權瀏覽/列印電子全文服務,2018-02-02起公開。zh_TW
dc.subjectPhalaenopsisen_US
dc.subjectgenetic diversityen_US
dc.subjectmolecular markeren_US
dc.subjectphylogeneticen_US
dc.subjectISSRen_US
dc.subjectSRAPen_US
dc.subject蝴蝶蘭屬zh_TW
dc.subject遺傳歧異度zh_TW
dc.subject分子標誌zh_TW
dc.subject親緣關係zh_TW
dc.subject植物分類zh_TW
dc.subjectISSRzh_TW
dc.subjectSRAPzh_TW
dc.title以形態、ISSR及SRAP分子標誌分析蝴蝶蘭屬物種之遺傳歧異度zh_TW
dc.titleGenetic Diversity Analysis of Phalaenopsis Species Based on Morphologic Characters, ISSR and SRAP Markers.en_US
dc.typeThesis and Dissertationen_US
dc.date.paperformatopenaccess2018-02-02zh_TW
dc.date.openaccess2018-02-02-
item.languageiso639-1zh_TW-
item.openairetypeThesis and Dissertation-
item.cerifentitytypePublications-
item.grantfulltextrestricted-
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item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
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