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dc.contributor.authorYa-Jyun Shenen_US
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dc.description.abstractTo investigate the genetic diversity among genera Phalaenopsis species, morphology characters, ISSR and SRAP marker were performed in this study. The performance of morphology characters, ISSR and SRAP markers, single or in combination for genetic diversity assessment and classification were similar. In summary, samples from the subgenus Parishianae were separate clustered different from other taxonomic status, and samples form the four section Polychilos, Fuscatae, Amboinensis and Zebrinae of subgenus Polychilos were frequently clustered together in, this indicated that the genetic diversity among each section of this subgenus were similar, then samples from section Phalaenopsis and Stauroglottis of subgenus Phalaenopsis often had a similar genetic distance and different from section Deliciosa and Esmeralda of subgenus Phalaenopsis. Finally, samples from subgenus Aphyllae were frequently clustered together with other taxonomic status, showed closely relate. This study showed that both ISSR and SRAP markers could be an efficient tool for study genetic diversity and relationship of genus Phalaenopsis kinship native orchid species, this could contribute germplasm conservation and breeding native orchid species in the future.en_US
dc.description.tableofcontents章 節 中文摘要 i 英文摘要 ii 目 錄 1 表 目 錄 3 圖 目 錄 4 附 錄 5 第一章 緒 論 7 第二章 前人研究 8 一、 蝴蝶蘭屬植物分類學地位、地理分布及形態概述 8 二、 分子標誌在遺傳研究之應用 8 (一) 簡單序列區間重複(inter-simple sequence repeat, ISSR) 9 (二) 相關序列增幅多型性(sequence-related amplified polymorphism, SRAP) 10 (三) 不同分子標誌之遺傳歧異度分析能力 10 三、 蝴蝶蘭屬分群分類之研究 11 (一) 形態學研究 11 (二) 細胞遺傳學與DNA含量 12 (三) 分子標誌 12 第三章 材料方法 14 一、 試驗材料 14 二、 形態特徵調查 14 (一) 葉部特徵(characteristics of leaf) 15 (二) 花梗特徵(characteristics of inflorescence) 15 (三) 花部特徵(characteristics of flower) 15 (四) 上萼瓣特徵(characteristics of dorsal sepal) 15 (五) 下萼瓣特徵(characteristics of lateral sepal) 16 (六) 翼瓣特徵(characteristics of petal) 16 (七) 唇瓣特徵(characteristics of lip) 17 (八) 中央裂片特徵(characteristics of apical lobe) 17 (九) 側裂片特徵(characteristics of lateral lobe) 18 三、 DNA萃取作業 18 四、 分子標誌之反應條件 19 五、 膠體分析 19 六、 統計分析 20 第四章 結 果 21 一、 形態特徵調查分析結果 21 二、 DNA萃取作業 22 三、 ISSR分子標誌分析結果 22 四、 SRAP分子標誌分析結果 23 五、 合併ISSR與SRAP分子標誌資料之分析結果 24 第五章 討 論 25 一、 DNA萃取作業 25 二、 形態特徵之分析 26 三、 比較兩種分子標誌之分析能力 27 四、 綜合形態特徵、地理分布、DNA含量及分子證據結果 28 第六章 結 論 30 第七章 參考文獻 31zh_TW
dc.subjectgenetic diversityen_US
dc.subjectmolecular markeren_US
dc.titleGenetic Diversity Analysis of Phalaenopsis Species Based on Morphologic Characters, ISSR and SRAP Markers.en_US
dc.typeThesis and Dissertationen_US
item.openairetypeThesis and Dissertation-
item.fulltextwith fulltext-
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