Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/89554
標題: 利用分子標誌輔助選拔育成抗白葉枯病之水稻新品種
Development of new varieties with bacterial blight resistance genes by using marker-assisted selection
作者: Che-Fu Chang
張哲輔
關鍵字: rice;bacterial blight;marker-assisted selection;水稻;白葉枯病;分子標誌輔助選拔
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摘要: 
水稻白葉枯病是由白葉枯病菌Xanthomonas oryzae pv. oryzae (Xoo)所感染的系統性病害,白葉枯病可導致水稻產量減少20 %至50 %,目前大多使用農藥防治,但對環境造成影響,且防治效果有限。前人研究顯示,在植株內堆疊多個不同抗性機制的抗性基因能抵抗更多生理小種,達到廣幅抗性。台南11號 (TN11) 具有高產、抗倒伏、抗褐飛蝨等優良特性,是目前台灣種植面積最廣的品種,但其對白葉枯病不具有抗性。本研究以國際水稻研究所 (International Rice Research Institute, IRRI) 育成帶有五個抗白葉枯病基因 (Xa4+xa5+Xa7+xa13+Xa21) 的秈稻品種IRBB66作為抗病基因提供親 (donor parent),與稉稻TN11進行雜交和回交,導入白葉枯病抗性基因,再以分子標誌輔助選拔法 (marker- assisted selection,MAS) 選出帶有五個抗性基因的植株後,自交純化,並進行外表型選拔,希望選出抗白葉枯病且農藝性狀良好之植株,以育成優良抗病品種。本試驗使用BC1及BC2兩個回交族群,不同回交世代在外表性狀上會有更多的選擇,從BC1F2與BC2F2世代始,以與抗病基因連鎖之分子標誌篩選帶有四個 (xa5+Xa7+xa13+Xa21) 或五個抗病基因的植株,於植株最高分蘗期接種病原菌進行抗病檢定,透過農藝性狀篩選後,選拔優良植株於下一期作進行抗病基因和外表性狀純化,並繼續篩選。至2014Ⅱ期作,選育出的18個抗病品系已達BC1F5及BC2F6世代,種植至苗栗改良場、霧峰農試所與北溝農場並接種Xoof2、Xf89-b及Xe2三種病原菌,檢定結果呈抗性反應,且植株外觀、米質及產量表現良好,抗褐飛蝨又抗倒伏,未來可推廣成為抗白葉枯病的新品種,亦可做為良好的雜交親本。本試驗亦利用禾本科網站中,比較圖譜的資訊進行圖譜交叉比對,並經由親本間多型性檢定,搜尋到位在抗病基因上和與抗病基因連鎖距離更近之SSR分子標誌,不僅可節省成本及時間的耗費,更有利於育種效率的提升。
URI: http://hdl.handle.net/11455/89554
其他識別: U0005-0202201512193600
Rights: 同意授權瀏覽/列印電子全文服務,2017-02-05起公開。
Appears in Collections:農藝學系

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