Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/90099
標題: Identification of a Chlorophyll Salvage Pathway and a Novel Chlorophyllase in Arabidopsis
阿拉伯芥葉綠素回收再利用途徑與一個新穎葉綠素酶的發現和探討
作者: 林耀斌
Yao-Pin Lin
關鍵字: 葉綠素回收再利用;葉綠素降解;葉綠素合成酶;葉綠素酶;chlorophyll turnover;chlorophyll salvage;chlorophyll synthase;chlorophyllase
引用: Allakhverdiev S, Kreslavski V, Klimov V, Los D, Carpentier R, Mohanty P (2008) Heat stress: an overview of molecular responses in photosynthesis. Photosynthesis Research 98: 541-550 Allakhverdiev SI, Nishiyama Y, Miyairi S, Yamamoto H, Inagaki N, Kanesaki Y, Murata N (2002) Salt Stress Inhibits the Repair of Photodamaged Photosystem II by Suppressing the Transcription and Translation of psbAGenes in Synechocystis. Plant Physiology 130: 1443-1453 Alonso JM, Stepanova AN, Leisse TJ, et a (2003) Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301: 653-657 Arkus KAJ, Cahoon EB, Jez JM (2005) Mechanistic analysis of wheat chlorophyllase. Archives of Biochemistry and Biophysics 438: 146-155 Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta Vulgaris. Plant Physiol 24: 1-15 Aro E-M, Suorsa M, Rokka A, Allahverdiyeva Y, Paakkarinen V, Saleem A, Battchikova N, Rintamaki E (2005) Dynamics of photosystem II: a proteomic approach to thylakoid protein complexes. Journal of Experimental Botany 56: 347-356 Aro E-M, Virgin I, Andersson B (1993) Photoinhibition of Photosystem II. Inactivation, protein damage and turnover. Biochimica et Biophysica Acta (BBA) - Bioenergetics 1143: 113-134 Azoulay Shemer T, Harpaz-Saad S, Belausov E, Lovat N, Krokhin O, Spicer V, Standing KG, Goldschmidt EE, Eyal Y (2008) Citrus Chlorophyllase Dynamics at Ethylene-Induced Fruit Color-Break: A Study of Chlorophyllase Expression, Posttranslational Processing Kinetics, and in Situ Intracellular Localization. Plant Physiology 148: 108-118 Buchert AM, Civello PM, Martinez GA (2011) Chlorophyllase versus pheophytinase as candidates for chlorophyll dephytilation during senescence of broccoli. Journal of Plant Physiology 168: 337-343 Banaś AK, Łabuz J, Sztatelman O, Gabryś H, Fiedor L (2011) Expression of Enzymes Involved in Chlorophyll Catabolism in Arabidopsis Is Light Controlled. Plant Physiology 157: 1497-1504 Beisel KG, Jahnke S, Hofmann D, Koppchen S, Schurr U, Matsubara S (2010) Continuous Turnover of Carotenes and Chlorophyll a in Mature Leaves of Arabidopsis Revealed by 14CO2 Pulse-Chase Labeling. Plant Physiology 152: 2188-2199 Berg JM, Tymoczko JL, L LS (2002) Light Absorption by Chlorophyll Induces Electron Transfer, Ed 5th Vol 19.2. W H Freeman, New York Betterle N, Ballottari M, Zorzan S, de Bianchi S, Cazzaniga S, Dall'Osto L, Morosinotto T, Bassi R (2009) Light-induced Dissociation of an Antenna Hetero-oligomer Is Needed for Non-photochemical Quenching Induction. Journal of Biological Chemistry 284: 15255-15266 Brzezowski P, Grimm B (2013) Chlorophyll Metabolism. In eLS. John Wiley & Sons, Ltd Charng Y-y, Liu H-c, Liu N-y, Chi W-t, Wang C-n, Chang S-h, Wang T-t (2007) A Heat-Inducible Transcription Factor, HsfA2, Is Required for Extension of Acquired Thermotolerance in Arabidopsis. Plant Physiology 143: 251-262 Charng Y-y, Liu H-c, Liu N-y, Hsu F-c, Ko S-s (2006) Arabidopsis Hsa32, a Novel Heat Shock Protein, Is Essential for Acquired Thermotolerance during Long Recovery after Acclimation. Plant Physiology 140: 1297-1305 Chidgey JW, Linhartova M, Komenda J, Jackson PJ, Dickman MJ, Canniffe DP, Konik P, Pilny J, Hunter CN, Sobotka R (2014) A cyanobacterial chlorophyll synthase-HliD complex associates with the Ycf39 protein and the YidC/Alb3 insertase. Plant Cell 26: 1267-1279 Chu C-C, Li H-m (2011) Determining the location of an Arabidopsis chloroplast protein using in vitro import followed by fractionation and alkaline extraction. In RP Jarvis, ed, Methods in Mol. Bio. - Chloroplast Res. in Arabidopsis, Vol 774. Humana Press, pp 339-350 Crocker W (1913) Chloroplasts and Chlorophyll. Botanical Gazette 56: 164-166 D'haeseleer P, Liang S, Somogyi R (2000) Genetic network inference: from co-expression clustering to reverse engineering. Bioinformatics 16: 707-726 Dodson G, Wlodawer A (1998) Catalytic triads and their relatives. Trends in Biochemical Sciences 23: 347-352 Eckhardt U, Grimm B, Hortensteiner S (2004) Recent advances in chlorophyll biosynthesis and breakdown in higher plants. Plant Molecular Biology 56: 1-14 Edelman M, Mattoo A (2008) D1-protein dynamics in photosystem II: the lingering enigma. Photosynthesis Research 98: 609-620 Emanuelsson O, Nielsen H, Von Heijne G (1999) ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites. Protein Science 8: 978-984 Espineda CE, Linford AS, Devine D, Brusslan JA (1999) The AtCAO gene, encoding chlorophyll a oxygenase, is required for chlorophyll b synthesis in Arabidopsis thaliana. Proceedings of the National Academy of Sciences of the United States of America 96: 10507-10511 Falbel TG, Staehelin LA (1994) Characterization of a family of chlorophyll-deficient wheat (Triticum) and barley (Hordeum vulgare) mutants with defects in the magnesium-insertion step of chlorophyll biosynthesis. Plant Physiol. 104: 639-648 Feierabend J, Dehne S (1996) Fate of the porphyrin cofactors during the light-dependent turnover of catalase and of the photosystem II reaction-center protein D1 in mature rye leaves. Planta 198: 413-422 Ferro M, Brugiere S, Salvi D, Seigneurin-Berny D, Court M, Moyet L, Ramus C, Miras S, Mellal M, Le Gall S, Kieffer-Jaquinod S, Bruley C, Garin J, Joyard J, Masselon C, Rolland N (2010) AT_CHLORO, a Comprehensive Chloroplast Proteome Database with Subplastidial Localization and Curated Information on Envelope Proteins. Molecular & Cellular Proteomics 9: 1063-1084 Garrido JL, Rodr'ıguez F, Campan˜a E, Zapata M (2003) Rapid separation of chlorophylls a and b and their demetallated and dephytylated derivatives using a monolithic silica C18 column and a pyridine-containing mobile phase. Journal of Chromatography A 994: 85-92 Gaubier P, Wu HJ, Laudie M, Delseny M, Grellet F (1995) A chlorophyll synthetase gene from Arabidopsis thaliana. Molecular and General Genetics 249: 58-64 Gombos Z, Wada H, Murata N (1994) The recovery of photosynthesis from low-temperature photoinhibition is accelerated by the unsaturation of membrane lipids: a mechanism of chilling tolerance. Proceedings of the National Academy of Sciences 91: 8787-8791 Griffiths WT (1978) Reconstitution of chlorophyllide formation by isolated etioplast membranes. Biochemical Journal 174: 681-692 Guyer L, Hofstetter SS, Christ B, Lira BS, Rossi M, Hortensteiner S (2014) Different Mechanisms Are Responsible for Chlorophyll Dephytylation during Fruit Ripening and Leaf Senescence in Tomato. Plant Physiology 166: 44-56 Hortensteiner S (2006) Chlorophyll Degradation During Senescence. Annual Review of Plant Biology 57: 55-77 Hortensteiner S (2013) The pathway of chlorophyll degradation: catabolites, enzymes and pathway regulation. In Plastid Development in Leaves during Growth and Senescence, Vol 36. Springer Netherlands, pp 363-392 Harpaz-Saad S, Azoulay T, Arazi T, Ben-Yaakov E, Mett A, Shiboleth YM, Hortensteiner S, Gidoni D, Gal-On A, Goldschmidt EE, Eyal Y (2007) Chlorophyllase Is a Rate-Limiting Enzyme in Chlorophyll Catabolism and Is Posttranslationally Regulated. Plant Cell 19: 1007-1022 Hayashi K, Hashimoto N, Daigen M, Ashikawa I (2004) Development of PCR-based SNP markers for rice blast resistance genes at the Piz locus. Theoretical and Applied Genetics 108: 1212-1220 Helliwell C, Waterhouse P (2003) Constructs and methods for high-throughput gene silencing in plants. Methods 30: 289-295 Hemsley A, Arnheim N, Toney MD, Cortopassi G, Galas DJ (1989) A simple method for site-directed mutagenesis using the polymerase chain reaction. Nucleic Acids Research 17: 6545-6551 Hirashima M, Tanaka R, Tanaka A (2009) Light-Independent Cell Death Induced by Accumulation of Pheophorbide. Plant and Cell Physiology 50: 719-729 Holden M (1961) The breakdown of chlorophyll by chlorophyllase. Biochemical Journal 78: 359-364 Horie Y, Ito H, Kusaba M, Tanaka R, Tanaka A (2009) Participation of Chlorophyll b Reductase in the Initial Step of the Degradation of Light-harvesting Chlorophyll a/b-Protein Complexes in Arabidopsis. Journal of Biological Chemistry 284: 17449-17456 Hu C, Lin S-y, Chi W-t, Charng Y-y (2012) Recent Gene Duplication and Subfunctionalization Produced a Mitochondrial GrpE, the Nucleotide Exchange Factor of the Hsp70 Complex, Specialized in Thermotolerance to Chronic Heat Stress in Arabidopsis. Plant Physiology 158: 747-758 Hu X, Tanaka A, Tanaka R (2013) Simple extraction methods that prevent the artifactual conversion of chlorophyll to chlorophyllide during pigment isolation from leaf samples. Plant Methods 9: 19-31 Hukmani P, Tripathy BC (1992) Spectrofluorometric estimation of intermediatesof chlorophyll biosynthesis: Protoporphyrin IX, Mg-protoporphyrin, and protochlorophyllide. Analytical Biochemistry 206: 125-130 Ischebeck T, Zbierzak AM, Kanwischer M, Dormann P (2006) A Salvage Pathway for Phytol Metabolism in Arabidopsis. Journal of Biological Chemistry 281: 2470-2477 Jarvi S, Suorsa M, Paakkarinen V, Aro EM (2011) Optimized native gel systems for separation of thylakoid protein complexes: novel super- and mega-complexes. Biochemical Journal 439: 207-214 Jacob-Wilk D, Holland D, Goldschmidt EE, Riov J, Eyal Y (1999) Chlorophyll breakdown by chlorophyllase: isolation and functional expression of the Chlase1 gene from ethylene-treated Citrus fruit and its regulation during development. The Plant Journal 20: 653-661 Jander G (2006) Gene identification and cloning by molecular marker mapping. In Arabidopsis Protocols, pp 115-126 Kariola T, Brader G, Li J, Palva ET (2005) Chlorophyllase 1, a damage control enzyme, affects the balance between defense pathways in plants. Plant Cell 17: 282-294 Kim S, Schlicke H, Van Ree K, Karvonen K, Subramaniam A, Richter A, Grimm B, Braam J (2013) Arabidopsis chlorophyll biosynthesis: an essential balance between the methylerythritol phosphate and tetrapyrrole pathways. Plant Cell 25: 4984-4993 Komenda J, Sobotka R, Nixon PJ (2012) Assembling and maintaining the Photosystem II complex in chloroplasts and cyanobacteria. Current Opinion in Plant Biology 15: 245-251 Kopečna J, Komenda J, Bučinska L, Sobotka R (2012) Long-term acclimation of the cyanobacterium Synechocystis sp. PCC 6803 to high light Is accompanied by an enhanced production of chlorophyll that Is preferentially channeled to trimeric photosystem I. Plant Physiology 160: 2239-2250 Krieger-Liszkay A (2005) Singlet oxygen production in photosynthesis. Journal of Experimental Botany 56: 337-346 Lin Y-P, Lee T-y, Tanaka A, Charng Y-y (2014) Analysis of an Arabidopsis heat-sensitive mutant reveals that chlorophyll synthase is involved in reutilization of chlorophyllide during chlorophyll turnover. The Plant Journal 80: 14-26 Liu H-c, Charng Y-y (2013) Common and Distinct Functions of Arabidopsis Class A1 and A2 Heat Shock Factors in Diverse Abiotic Stress Responses and Development. Plant Physiology 163: 276-290 Liu H-C, Liao H-T, Charng Y-Y (2011) The role of class A1 heat shock factors (HSFA1s) in response to heat and other stresses in Arabidopsis. Plant, Cell & Environment 34: 738-751 Mene-Saffrane L, DellaPenna D (2010) Biosynthesis, regulation and functions of tocochromanols in plants. Plant Physiology and Biochemistry 48: 301-309 Malkin S, Armond PA, Mooney HA, Fork DC (1981) Photosystem II Photosynthetic Unit Sizes from Fluorescence Induction in Leaves: CORRELATION TO PHOTOSYNTHETIC CAPACITY. Plant Physiology 67: 570-579 Marutani Y, Yamauchi Y, Kimura Y, Mizutani M, Sugimoto Y (2012) Damage to photosystem II due to heat stress without light-driven electron flow: involvement of enhanced introduction of reducing power into thylakoid membranes. Planta 236: 753-761 Masuda T, Tanaka A, Melis A (2003) Chlorophyll antenna size adjustments by irradiance in Dunaliella salina involve coordinate regulation of chlorophyll a oxygenase (CAO) and Lhcb gene expression. Plant Molecular Biology 51: 757-771 Matile P, Hortensteiner S, Thomas H (1999) Chlorophyll degradation. Annual Review of Plant Physiology and Plant Molecular Biology 50: 67-95 McFeeters RF (1975) Substrate Specificity of Chlorophyllase. Plant Physiology 55: 377-381 McFeeters RF, Chichester CO, Whitaker JR (1971) Purification and Properties of Chlorophyllase from Ailanthus altissima (Tree-of-Heaven). Plant Physiology 47: 609-618 Melis A (1984) Light regulation of photosynthetic membrane structure, organization, and function. Journal of Cellular Biochemistry 24: 271-285 Meskauskiene R, Nater M, Goslings D, Kessler F, op den Camp R, Apel K (2001) FLU: A negative regulator of chlorophyll biosynthesis in Arabidopsisthaliana. Proceedings of the National Academy of Sciences of the United States of America 98: 12826-12831 Minagawa J, Takahashi Y (2004) Structure, function and assembly of Photosystem II and its light-harvesting proteins. Photosynthesis Research 82: 241-263 Morita R, Sato Y, Masuda Y, Nishimura M, Kusaba M (2009) Defect in non-yellow coloring 3, an α/β hydrolase-fold family protein, causes a stay-green phenotype during leaf senescence in rice. The Plant Journal 59: 940-952 Mur LAJ, Aubry S, Mondhe M, Kingston-Smith A, Gallagher J, Timms-Taravella E, James C, Papp I, Hortensteiner S, Thomas H, Ougham H (2010) Accumulation of chlorophyll catabolites photosensitizes the hypersensitive response elicited by Pseudomonas syringae in Arabidopsis. New Phytologist 188: 161-174 Murata N, Takahashi S, Nishiyama Y, Allakhverdiev SI (2007) Photoinhibition of photosystem II under environmental stress. Biochimica et Biophysica Acta (BBA) - Bioenergetics 1767: 414-421 Nakagawara E, Sakuraba Y, Yamasato A, Tanaka R, Tanaka A (2007) Clp protease controls chlorophyll b synthesis by regulating the level of chlorophyllide a oxygenase. The Plant Journal 49: 800-809 Nath K, Jajoo A, Poudyal RS, Timilsina R, Park YS, Aro E-M, Nam HG, Lee CH (2013) Towards a critical understanding of the photosystem II repair mechanism and its regulation during stress conditions. FEBS Letters 587: 3372-3381 Nelson N, Yocum CF (2006) Structure and function of photosystems I and II. Annual Review of Plant Biology 57: 521-565 Nishiyama Y, Allakhverdiev SI, Yamamoto H, Hayashi H, Murata N (2004) Singlet Oxygen Inhibits the Repair of Photosystem II by Suppressing the Translation Elongation of the D1 Protein in Synechocystis sp. PCC 6803+. Biochemistry 43: 11321-11330 Nixon PJ, Michoux F, Yu J, Boehm M, Komenda J (2010) Recent advances in understanding the assembly and repair of photosystem II. Annals of Botany 106: 1-16 Obayashi T, Kinoshita K, Nakai K, Shibaoka M, Hayashi S, Saeki M, Shibata D, Saito K, Ohta H (2007) ATTED-II: a database of co-expressed genes and cis elements for identifying co-regulated gene groups in Arabidopsis. Nucleic Acids Research 35: D863-D869 Oosawa N, Masuda T, Awai K, Fusada N, Shimada H, Ohta H, Takamiya K-i (2000) Identification and light-induced expression of a novel gene of NADPH-protochlorophyllide oxidoreductase isoform in Arabidopsis thaliana. FEBS Letters 474: 133-136 op den Camp RGL, Przybyla D, Ochsenbein C, Laloi C, Kim C, Danon A, Wagner D, Hideg E, Gobel C, Feussner I, Nater M, Apel K (2003) Rapid Induction of Distinct Stress Responses after the Release of Singlet Oxygen in Arabidopsis. Plant Cell 15: 2320-2332 Oster U, Rudiger W (1997) The G4 gene of Arabidopsis thaliana encodes a chlorophyll synthase of etiolated plants. Botanica Acta 110: 420-423 Oster U, Tanaka R, Tanaka A, Rudiger W (2000) Cloning and functional expression of the gene encoding the key enzyme for chlorophyll b biosynthesis (CAO) from Arabidopsis thaliana. The Plant Journal 21: 305-310 Pružinska A, Tanner G, Aubry S, Anders I, Moser S, Muller T, Ongania K-H, Krautler B, Youn J-Y, Liljegren SJ, Hortensteiner S (2005) Chlorophyll Breakdown in Senescent Arabidopsis Leaves. Characterization of Chlorophyll Catabolites and of Chlorophyll Catabolic Enzymes Involved in the Degreening Reaction. Plant Physiology 139: 52-63 Ragas X, Jimenez-Banzo A, Sanchez-Garcia D, Batllori X, Nonell S (2009) Singlet oxygen photosensitisation by the fluorescent probe Singlet Oxygen Sensor GreenR. Chemical Communications 20: 2920-2922 Raskin VI, Fleminger D, Marder JB (1995) Integration and turnover of photosystem II pigment. In Photosynthesis: from light to biosphere. Kluwer, pp 945-948 Riper DM, Owens TG, Falkowski PG (1979) Chlorophyll Turnover in Skeletonema costatum, a Marine Plankton Diatom. Plant Physiology 64: 49-54 Rise M, Cojocaru M, Gottlieb HE, Goldschmidt EE (1989) Accumulation of α-Tocopherol in Senescing Organs as Related to Chlorophyll Degradation. Plant Physiology 89: 1028-1030 Rossini L, Cribb L, Martin DJ, Langdale JA (2001) The maize Golden2 gene defines a novel class of transcriptional regulators in plants. Plant Cell 13: 1231-1244 Sakuraba Y, Schelbert S, Park S-Y, Han S-H, Lee B-D, Andres CB, Kessler F, Hortensteiner S, Paek N-C (2012) STAY-GREEN and Chlorophyll Catabolic Enzymes Interact at Light-Harvesting Complex II for Chlorophyll Detoxification during Leaf Senescence in Arabidopsis. Plant Cell 24: 507-518 Sato Y, Morita R, Katsuma S, Nishimura M, Tanaka A, Kusaba M (2009) Two short-chain dehydrogenase/reductases, NON-YELLOW COLORING 1 and NYC1-LIKE, are required for chlorophyll b and light-harvesting complex II degradation during senescence in rice. The Plant Journal 57: 120-131 Schelbert S, Aubry S, Burla B, Agne B, Kessler F, Krupinska K, Hortensteiner S (2009) Pheophytin pheophorbide hydrolase (pheophytinase) is involved in chlorophyll breakdown during leaf senescence in Arabidopsis. Plant Cell 21: 767-785 Schenk N, Schelbert S, Kanwischer M, Goldschmidt EE, Dormann P, Hortensteiner S (2007) The chlorophyllases AtCLH1 and AtCLH2 are not essential for senescence-related chlorophyll breakdown in Arabidopsis thaliana. FEBS Letters 581: 5517-5525 Shalygo N, Czarnecki O, Peter E, Grimm B (2009) Expression of chlorophyll synthase is also involved in feedback-control of chlorophyll biosynthesis. Plant Molecular Biology 71: 425-436 Shimada Y, Tanaka A, Tanaka Y, Takabe T, Takabe T, Tsuji H (1990) Formation of Chlorophyll-Protein Complexes during Greening 1. Distribution of Newly Synthesized Chlorophyll among Apoproteins. Plant and Cell Physiology 31: 639-647 Sun Q, Zybailov B, Majeran W, Friso G, Olinares PDB, van Wijk KJ (2009) PPDB, the Plant Proteomics Database at Cornell. Nucleic Acids Research 37: D969-D974 Takabayashi A, Kadoya R, Kuwano M, Kurihara K, Ito H, Tanaka R, Tanaka A (2013) Protein co-migration database (PCoM -DB) for Arabidopsis thylakoids and Synechocystis cells. SpringerPlus 2: 1-10 Takamiya K-i, Tsuchiya T, Ohta H (2000) Degradation pathway(s) of chlorophyll: what has gene cloning revealed? Trends in Plant Science 5: 426-431 Tanaka A, Tanaka Y, Takabe T, Tsuji H (1995) Calcium-induced accumulation of apoproteins of the light-harvesting chlorophyll ab-protein complex in cucumber cotyledons in the dark. Plant Science 105: 189-194 Tanaka R, Ito H, Tanaka A (2010) Regulation and Functions of the Chlorophyll Cycle. In CA Rebeiz, C Benning, HJ Bohnert, H Daniell, JK Hoober, HK Lichtenthaler, AR Portis, BC Tripathy, eds, The Chloroplast, Vol 31. Springer Netherlands, pp 55-78 Tanaka R, Kobayashi K, Masuda T (2011) Tetrapyrrole Metabolism in Arabidopsis thaliana. The Arabidopsis Book 94: e0145 Tanaka R, Koshino Y, Sawa S, Ishiguro S, Okada K, Tanaka A (2001) Overexpression of chlorophyllide a oxygenase (CAO) enlarges the antenna size of photosystem II in Arabidopsis thaliana. The Plant Journal 26: 365-373 Tanaka R, Rothbart M, Oka S, Takabayashi A, Takahashi K, Shibata M, Myouga F, Motohashi R, Shinozaki K, Grimm B, Tanaka A (2010) LIL3, a light-harvesting-like protein, plays an essential role in chlorophyll and tocopherol biosynthesis. Proceedings of the National Academy of Sciences 107: 16721-16725 Tanaka R, Tanaka A (2011) Chlorophyll cycle regulates the construction and destruction of the light-harvesting complexes. Biochimica et Biophysica Acta (BBA) - Bioenergetics 1807: 968-976 Tripathy BC, Pattanayak GK (2012) Chlorophyll Biosynthesis in Higher Plants. In Photosynthesis, Vol 34. Springer Netherlands, pp 63-94 Tsuchiya T, Ohta H, Okawa K, Iwamatsu A, Shimada H, Masuda T, Takamiya K-i (1999) Cloning of chlorophyllase, the key enzyme in chlorophyll degradation: Finding of a lipase motif and the induction by methyl jasmonate. Porc. Natl. Acad. Sci. USA 96: 15362-15367 Tsuchiya T, Ohta H, Okawa K, Iwamatsu A, Shimada H, Masuda T, Takamiya K-i (1999) Cloning of chlorophyllase, the key enzyme in chlorophyll degradation: Finding of a lipase motif and the induction by methyl jasmonate. Proceedings of the National Academy of Sciences 96: 15362-15367 Tsuchiya T, Suzuki T, Yamada T, Shimada H, Masuda T, Ohta H, Takamiya K-i (2003) Chlorophyllase as a Serine Hydrolase: Identification of a Putative Catalytic Triad. Plant and Cell Physiology 44: 96-101 Valentin HE, Lincoln K, Moshiri F, Jensen PK, Qi Q, Venkatesh TV, Karunanandaa B, Baszis SR, Norris SR, Savidge B, Gruys KJ, Last RL (2006) The Arabidopsis vitamin E pathway gene5-1 Mutant Reveals a Critical Role for Phytol Kinase in Seed Tocopherol Biosynthesis. Plant Cell 18: 212-224 Vavilin D, Vermaas W (2007) Continuous chlorophyll degradation accompanied by chlorophyllide and phytol reutilization for chlorophyll synthesis in Synechocystis sp. PCC 6803. Biochimica et Biophysica Acta (BBA) - Bioenergetics 1767: 920-929 Warren MJ, Smith AG, Rudiger W (2009) Regulation of the late steps of chlorophyll biosynthesis. In Tetrapyrroles. Springer New York, pp 263-273 Waters MT, Wang P, Korkaric M, Capper RG, Saunders NJ, Langdale JA (2009) GLK transcription factors coordinate expression of the photosynthetic apparatus in Arabidopsis. Plant Cell 21: 1109-1128 Willstatter R, Stoll A (1913) Untersuchungen uber Chlorophyll. Verlag Julius Springer, Berlin Winter D, Vinegar B, Nahal H, Ammar R, Wilson GV, Provart NJ (2007) An 'Electronic Fluorescent Pictograph' browser for exploring and analyzing large-scale biological data sets. PLoS ONE 2: e718 Wolken JJ, Schwertz FA (1953) CHLOROPHYLL MONOLAYERS IN CHLOROPLASTS. The Journal of General Physiology 37: 111-120 Wu T-y, Juan Y-t, Hsu Y-h, Wu S-h, Liao H-t, Fung RWM, Charng Y-y (2013) Interplay between Heat Shock Proteins, HSP101 and HSA32, Prolongs Heat Acclimation Memory Posttranscriptionally in Arabidopsis. Plant Physiol. 161: 2075-2084 Wu Z, Zhang X, He B, Diao L, Sheng S, Wang J, Guo X, Su N, Wang L, Jiang L, Wang C, Zhai H, Wan J (2007) A Chlorophyll-Deficient Rice Mutant with Impaired Chlorophyllide Esterification in Chlorophyll Biosynthesis. Plant Physiology 145: 29-40 Yamasato A, Nagata N, Tanaka R, Tanakaa A (2005) The N-Terminal Domain of Chlorophyllide a Oxygenase Confers Protein Instability in Response to Chlorophyll b Accumulation in Arabidopsis Plant Cell 17: 1585–1597 Yang M, Wardzala E, Johal G, Gray J (2004) The Wound-Inducible Lls1 Gene from Maize is an Orthologue of the Arabidopsis Acd1 Gene, and the LLS1 Protein is Present in Non-Photosynthetic Tissues. Plant Molecular Biology 54: 175-191 Yang W, Cahoon RE, Hunter SC, Zhang C, Han J, Borgschulte T, Cahoon EB (2011) Vitamin E biosynthesis: functional characterization of the monocot homogentisate geranylgeranyl transferase. The Plant Journal 65: 206-217 Zhang W, Liu T, Ren G, Hortensteiner S, Zhou Y, Cahoon EB, Zhang C (2014) Chlorophyll degradation: the tocopherol biosynthesis related phytol hydrolase in Arabidopsis seeds is still missing. Plant Physiology Zouni A, Witt H-T, Kern J, Fromme P, Krauss N, Saenger W, Orth P (2001) Crystal structure of photosystem II from Synechococcus elongatus at 3.8 A resolution. Nature 409: 739-743
摘要: 
Chlorophylls, which reflect green color after absorbing visible light, are the most abundant pigments in nature. They can interact with chlorophyll (Chl)-binding proteins to form photosystems for the progression of photosynthesis, the process by which sunlight is captured for the production of biochemical energy that supports most life on earth. Most chlorophyll intermediates, enzymes, and reactions included in chlorophyll anabolism and catabolism have been well-studied and characterized, as has the entire pathway. But the details about chlorophyll turnover and the chlorophyllase(s) necessary for the initiation of this metabolism in plants during the developmental stages and in response to environmental stimuli are not clear. In this study, two heat sensitive mutants that encode a mutated chlorophyll synthase and a putative chlorophyllase, respectively, in Arabidopsis were isolated using a forward genetic approach. Genetic and biochemical assays of the isolated chlorophyll synthase mutant in which salvage is prohibited, led to the proposal of a chlorophyll salvage model to illustrate chlorophyll a turnover and the recycling of chlorophyllide a during photosystem II repair under heat stress. The second mutant showed in vitro specificity in chlorophyll a and b dephytylation, but not pheophytin. This activity was also present in the wild-type form with lower catalytic ability and, therefore, this enzyme was functionally assigned to be a novel chlorophyllase distinct from the previous isolated chlorophyllase. The role of this novel chlorophyllase in the chlorophyll metabolism pathway, including in the above mentioned chlorophyll salvage, is dissected further with epistatic analysis.
URI: http://hdl.handle.net/11455/90099
Rights: 同意授權瀏覽/列印電子全文服務,2017-02-06起公開。
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