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標題: 建立免疫奈米金球之側層流檢測平臺以快速偵測牛奶中的仙人掌桿菌的汙染
Developing an immunogold nanoparticle-based lateral flow assay for the rapid detection of Bacillus cereus in milk
作者: 許萍芸
Ping-Yun Hsu
關鍵字: Polyclonal antibody;Bacillus cereus;immunogold nanoparticles;lateral flow assay;多株抗體;仙人掌桿菌;免疫奈米金球;側層流試片
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Since foodborne diseases and outbreaks increase considerably, food safety is always a widely held concern with respect to human health in the world. According to the suevey results in 2007 to 2012 from the Center for Disease Cotrol (CDC) of the Department of Health in Taiwan, Bacillus cereus was rated as a common pathoge in food poisoning, with approximately 17%patients were infected. Instead of conventional detecting methods for which require time, skilled technicians and special equipment, lateral flow assay (LFA) utilizing the specific binding between antigen and antibody is currently as an on-site detection tool applied in the realm of environmental, food and clinical analyses. Therefore, in this study, a rapid, simple, snesitive and specific LFA strip using polyclonal antibody we raised against 28.5-kDa cell surface protein of B. cereus and 30-nm immunogold nanoparticles had been successfully deveoped for the detection of B. cereus in milk. Whole assay could be completed within 20min. The visual detection limit (VDL) was determined as 10^3 CFU/mL with the dynamic range from 10^4 to 10^8 CFU/mL in both phosphate buffer (PB) system and milk samples, showing a high sensitivity to B.cereus. The specificity of LFA was also good, despite a little reactivity with Vibrio parahaemolyticus. The semi-quantitative results of the strip assay were in a good agreement with those of conventional spread plate method. In the precision analysis, the coefficients of variation (CV) of intra- and inter-assay were 9.6% and 12.8%, indicating hogh result reproducibility. Hence, the polyclonal antibody-based LFA platform we developed has a great potential as a user-friendly, rapid and sensitive on-site tool for detecting B. cereus in milk. within 20min. The visual detection limit (VDL) was determined as 10^3 CFU/mL with the dynamic range from 10^4 to 10^8 CFU/mL in both phosphate buffer (PB) system and milk samples, showing a high sensitivity to B.cereus. The specificity of LFA was also good, despite a little reactivity with Vibrio parahaemolyticus. The semi-quantitative results of the strip assay were in a good agreement with those of conventional spread plate method. In the precision analysis, the coefficients of variation (CV) of intra- and inter-assay were 9.6% and 12.8%, indicating hogh result reproducibility. Hence, the polyclonal antibody-based LFA platform we developed has a great potential as a user-friendly, rapid and sensitive on-site tool for detecting B. cereus in milk.
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