Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/90348
標題: 以ISSR分子標記檢測宜蘭月桃(Alpinia x ilanensis)為日本月桃(A. japonica)與普來氏月桃(A. pricei)雜交之假說
Testing a hypothetical hybrid, Alpinia x ilanensis, and its putative parents, A. japonica and A. pricei using Inter-Simple Sequence Repeats (ISSR) markers
作者: 祖帝愛
Diaiti Zure
關鍵字: NA;Alpina;hybridization;Inter-Simple Sequence Repeats (ISSR);species-specific markers;morphological traits
引用: Althoff, D. M., Gitzendanner, M. A., & Segraves, K. A. (2007). The utility of amplified fragment length polymorphisms in phylogenetics: a comparison of homology within and between genomes. Systematic biology, 56(3), 477-484. Arnold, M. L. (2004). Transfer and origin of adaptations through natural hybridization: were Anderson and Stebbins right?. The Plant Cell, 16(3), 562-570. Bachmann, K. (1994). Tansley Review No. 63. Molecular Markers in Plant ecology. New Phytologist , 403-418. Baker, J. G. (1894). Order CXLIX. Scitamineae. In J. D. Hooker [ed.], Flora of British India, vol. VI, Orchideae to Cyperaceae, 198–264. L. Reeve & Co., London, UK Baker, H.G., & Baker, I. (1979). Starch in angiosperm pollen grains and its evolutionary significance. American Journal of Botany 66: 591–600. Bardakci, F. (2001). Random amplified polymorphic DNA (RAPD) markers. Turk J Biol, 25(1), 2185-96. Carvalho, A., M. Matos, Lima-Brito, J., Guedes-Pinto, H., & Benito, C., (2005). DNA fingerprint of F1 interspecific hybrids from the Triticeae tribe using ISSRs. Euphytica 143, 93—99. Cato, S. A., & Richardson, T. E. (1996). Inter-and intraspecific polymorphism at chloroplast SSR loci and the inheritance of plastids in Pinus radiata D. Don. Theoretical and Applied Genetics, 93(4), 587-592. Dai, Ting-Xuan. (2012). The application of molecular markers in two spice and aromatic plants [Master Thesis]. Taichung, Taiwan: National Chung Hsing University. Davey, J. W., Hohenlohe, P. A., Etter, P. D., Boone, J. Q., Catchen, J. M., & Blaxter, M. L. (2011). Genome-wide genetic marker discovery and genotyping using next-generation sequencing. Nature Reviews Genetics, 12(7), 499-510. Ellstrand, N. C., Whitkus, R., & Rieseberg, L. H. (1996). Distribution of spontaneous plant hybrids. Proceedings of the National Academy of Sciences, 93(10), 5090-5093. Grant, V. (1981). Plant Speciation. Columbia University Press, New York. Gupta, P. K. & Varshney, R. K. (2000). The development and use of microsatellite markers forgenetic analysis and plant breeding with emphasis on bread wheat. Euphytica 113:163-185. Hammer, Ø., Harper, D. A. T., & Ryan, P. D. (2001). PAST-Palaeontological statistics. www. uv. es/~ pardomv/pe/2001_1/past/pastprog/past. pdf, acessado em, 25(07), 2009. Hegarty, M. J., & Hiscock, S. J. (2005). Hybrid speciation in plants: new insights from molecular studies. New Phytologist, 165(2), 411-423. Holttum, R. E. (1950). The zingiberaceae of the Malay Peninsula. Gard. Bull., Singapore, 13(1), 1-249. Jaccard, P. (1912). The distribution of the flora in the alpine zone. New phytologist, 11(2), 37-50. Kress, W. J., Liu, A. Z., Newman, M., & Li, Q. J. (2005). The molecular phylogeny of Alpinia (Zingiberaceae): a complex and polyphyletic genus of gingers. American Journal of Botany, 92(1), 167-178. Kress, W. J., Prince, L. M., & Williams, K. J. (2002). The phylogeny and a new classification of the gingers (Zingiberaceae): evidence from molecular data. American Journal of Botany, 89(10), 1682-1696. Kuo, Y.W. (2006). Taxonomy of the Genus Alpinia (Zingiberaceae) in Taiwan and the analyses of their rootstock essential oils [Master thesis]. Chiayi, Taiwan: Graduate institute of Forestry and Natural Resources, National Chiayi University. 127 p. Kuo, Y.-W., Lu, F.Y., Duh, M.H. & Deng, S. L. (2008). A new species, Alpinia nantoensis (Zingiberaceae) from Taiwan. Taiwan J. For. Sci. 23: 93-97. Lin, X. C., Lou, Y. F., Liu, J., Peng, J. S., Liao, G. L., & Fang, W. (2010). Crossbreeding of Phyllostachys species (Poaceae) and identification of their hybrids using ISSR markers. Genetics and molecular research, 9(3), 1398-1404. Linder, C. R., & Rieseberg, L. H. (2004). Reconstructing patterns of reticulate evolution in plants. American journal of botany, 91(10), 1700-1708. Liu, S.C, & Wang, J.C. (2009). New Natural Hybrid, Alpinia × ilanensis (Zingiberaceae) in Taiwan. Taiwania , 54 (2), 134-139. Liu, S. C., Lu, C. T., & Wang, J. C. (2009). Reticulate hybridization of Alpinia (Zingiberaceae) in Taiwan. Journal of plant research, 122(3), 305-316. Mallet, J. (2007). Hybrid speciation. Nature, 446(7133), 279-283. Mandal, A. K., & Gibson, G. L. (Eds.). (1998). Forest genetics and tree breeding. CBS Publishers & Distributors. Mao, X., Zhang, J., Zhang, S. & Rossiter, S. J. (2010). Historical male-mediated introgression in horseshoe bats revealed by multilocus DNA sequence data. Mol Ecol 19: 1352–1366. Meyer, W., Mitchell, T.G., Freedman, E.Z. & Vilgays, R. (1993). Hybridization probes for conventional DNA fingerprinting used as single primers in the polymerase chain reaction to distinguish strains of Cryptococcus neoformans. J Clin Microbiol 31: 2274–2280. Mian, M. A., Hopkins, A. A., & Zwonitzer, J. C. (2002). Determination of genetic diversity in tall fescue with AFLP markers. Crop Science, 42(3), 944-950. Moo, C.T. (1973). Taxonomic studies on Zingiberaceae plants of Taiwan [Master thesis]. Taipei, Taiwan: National Taiwan University. 183 p. Pavel and Vasile: PyElph - a software tool for gel images analysis and phylogenetics. BMC Bioinformatics 2012 13:9. Pinheiro, F., De Barros, F., Palma-Silva, C., Meyer, D., Fay, M. F., Suzuki, R. M., et al. (2010). Hybridization and introgression across different ploidy levels in the Neotropical orchids Epidendrum fulgens and E. puniceoluteum (Orchidaceae). Mol Ecol 19: 3981–3994. Reddy, M. P., Sarla, N., & Siddiq, E. A. (2002). Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica, 128(1), 9-17. Rieseberg, L. H. (1997). Hybrid origins of plant species. Annual review of Ecology and Systematics, 359-389. Rieseberg, L. H., Ellstrand, N. C., & Arnold, M. (1993). What can molecular and morphological markers tell us about plant hybridization?. Critical reviews in plant sciences, 12(3), 213-241. Rieseberg, L. H., Widmer, A., Arntz, A. M., & Burke, B. (2003). The genetic architecture necessary for transgressive segregation is common in both natural and domesticated populations. Philosophical Transactions of the Royal Society B: Biological Sciences, 358(1434), 1141-1147. Schlötterer, C. (2004). The evolution of molecular markers—just a matter of fashion?. Nature Reviews Genetics, 5(1), 63-69. Schumann, K. (1904). Zingiberaceae. Das Pflanzenreich, 4, 46. Searle, R. J., & Hedderson, T. A. J. (2000). A preliminary phylogeny of the Hedychieae tribe (Zingiberaceae) based on ITS sequences of the nuclear rRNA cistron. Monocots: systematics and evolution. Collingwood, Australia: CSIRO, 710-718. Simillion, C., Vandepoele., K., Van Montagu., M. C., Zabeau, M., & Van de Peer., Y. (2002). The hidden duplication past of Arabidopsis thaliana. Proceedings of the National Academy of Sciences, 99(21), 13627-13632. Smith, R. M. (1990). Alpinia (Zingiberaceae): a proposed new infrageneric classification. Edinburgh Journal of Botany, 47(01), 1-75. Soltis, E. D., & Soltis, P. S. (2000). Contributions of plant molecular systematics to studies of molecular evolution. Plant molecular biology, 42(1), 45-75. Su, Hsiang-Hao. (2013). Application of monocot SSR and ISSR markers on the genetic diversity analysis of Alpinia species [Master Thesis]. Taichung, Taiwan: National Chung Hsing University. Tautz, D. & M. Renz, (1984). Simple sequences are ubiquitous repetitive components of eukaryotic genomes. Nucl Acids Res 23:249–255. Twyford, A. D., & Ennos, R. A. (2012). Next-generation hybridization and introgression. Heredity, 108(3), 179-189. Ungerer, M. C., Baird, S. J., Pan, J., & Rieseberg, L. H. (1998). Rapid hybrid speciation in wild sunflowers. Proceedings of the National Academy of Sciences, 95(20), 11757-11762. Vos, P., Hogers, R., Bleeker, M., Reijans, M., Van de Lee, T., Hornes, M., ... & Zabeau, M. (1995). AFLP: a new technique for DNA fingerprinting. Nucleic acids research, 23(21), 4407-4414. Willyard, A., Cronn, R., & Liston, A. (2009). Reticulate evolution and incomplete lineage sorting among the ponderosa pines. Molecular Phylogenetics and Evolution, 52(2), 498-511. Wolfe, A. D., Xiang, Q. Y., & Kephart, S. R. (1998). Assessing hybridization in natural populations of Penstemon(Scrophulariaceae) using hypervariable intersimple sequence repeat(ISSR) bands. Molecular Ecology, 7(9), 1107-1125. Wood, T. H., Whitten, W. M., & Williams, N. H. (2000). Phylogeny of Hedychium and related genera (Zingiberaceae) based on ITS sequence data. Edinburgh Journal of Botany, 57(02), 261-270.and Pederson, 2004) Wu, K., Jones, R., Dannaeberger, L. & Scolnik, P.A. (1994). Detection of microsatellite polymorphisms without cloning. Nucleic Acids Res 22: 3257–3258. Wu, T. L. & K. Larsen. (2000). Zingiberaceae. In: Wu, Z.-Y. and P. H. Raven (eds.), Flora of China 24: 333-346. Sci. Press, Beijing, China. Yang, J. J., & Wang, J. C. (1998). The systematic study of Taiwanese Alpinia (Zingiberaceae). In Proceedings of the cross-strait symposium on floristic diversity and conservation. National Museum of Natural Science, Taichung, Taiwan (pp. 183-197). Yin, X., Stam, P., Dourleijn, C. J., & Kropff, M. J. (1999). AFLP mapping of quantitative trait loci for yield-determining physiological characters in spring barley. Theoretical and Applied Genetics, 99(1-2), 244-253. Zietkiewicz, E., Rafalski, A., and Labuda D. (1994). Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20: 176-183.
摘要: 
NA

This study tested a hypothetical hybrid, Alpinia x ilanensis and its putative parents, A. japonica and A. pricei discovered in the Northeast coast of Taiwan. The hybrid and its putative parents were previously studies based on morphological and eco-geographical evidences. It was revealed that the hybrid has characteristics that are variable but wholly in the range of its putative parents with exception to its other qualitative characters, which differs from the parents but discerns the intermediate state of hybrid. However, the direction of hybridization in the hybrid and its putative parent was unknown and it was hypothesized as a natural hybrid between its parents. Based on this hypothesis, we tested the hybrid and its putative parents using Inter-Simple Sequence Repeat (ISSR) markers. Selection of the markers for this study was based on its advantages combining Simple Sequence Repeats (SSR) and Amplified Fragmented Length Polymorphism (AFLP). Also, because the advantage of available species-specific primers of the putative parents from previous studies.
To test the hypothesis 8 A. japonica, 12 A. pricei and 3 A. x ilanensis were collected near Jiaoxi Township, Ilan County. Genomic DNA of the taxa were extracted from leaves tissue using a modified CTAB method, quantified and amplified with nine ISSR primers (UBC 807, 808, 811, 812, 818, 824, 836 & 840) respectively. ISSR-PCR products were screened under UV-transilluminator for DNA bands. Photos of individual amplicons with their duplicates were taken computed for their binary and molecular weight data using PyElp v.1.3 software. The binary data were loaded into excel spread to generated a binary matrix, which was used to calculate the polymorphism and the genetic relationship using Jaccard similarity coefficient and unweight pair group method with arithmetic average (UPGMA).
We found ISSR primers UBC807, 808, 811, 836 and 840 to share similar band patterns between the hybrids and putative parents. However, the relationship can only be established between the hybrid and A. japonica but not between the hybrid and A. pricei although hybrid individuals clustered together in the phylogenetic analysis results based on the marker genotypes and distinct from the other 8 A. japonica. Introgression is unlikely due to the hybrid is unfertile and therefore cannot backcross with its parent. We assume the possibilities that the taxa were probably misidentified or the hybrid is an offspring of another Alpinia species. For this study, we used leaf morphological traits to derive our findings. We proposed further verification of the hypothetical hybrid and its putative parent using reproductive trait and other molecular markers.
URI: http://hdl.handle.net/11455/90348
Rights: 同意授權瀏覽/列印電子全文服務,2015-08-20起公開。
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