Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/90589
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dc.contributorJyh-Myng Zenen_US
dc.contributor曾志明zh_TW
dc.contributor.author彭崇文zh_TW
dc.contributor.authorSong-Man Pangen_US
dc.contributor.other化學系所zh_TW
dc.date2015zh_TW
dc.date.accessioned2015-12-10T02:47:55Z-
dc.identifier.citation1. A.W.Knight, Trends, Anal. Chem 1999, 18, 47. 2. L. Hu , Chem. Soc. Rev. 2010, 39, 3275-3304. 3. 邱梅欣,國立中興大學化學系博士論文,98年。 4. 廖振唯,國立中興大學化學系博士論文,100年。 5. 魏瑋琤,國立中興大學化學系博士論文,101年。zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/90589-
dc.description.abstractThe development and application in Electrogenerated chemiluminescence (ECL) of fluorescent carbon nanoparticles (CNPs) is focused on this study. Which is prepared by electrochemical exfoliation of carbon fiber in a suitable solution condition with narrow size distribution, and good long-term stability. ECL signal of CNPs was observed in cyclic voltammetry(CV) as potential scanning between 0.4 to 2.3 V vs Ag/AgCl reference electrode and the ECL mechanisms of CNPs and CNPs-Ru(bpy)32+ were proposed. The cyclic voltammetry-generated chemiluminescent (CV-ECL) reactions of a series of aminoglycoside antibiotics were investigated in a flow system using carbon nanoparticle(CNPs)and Indium tin oxide(ITO) electrodes. The carbon nanoparticle were found to participate as an electrocatalyst in an oxidative-reductive ECL mechanism with tris(2,2'-bipyridyl)ruthenium(II) (Ru(bpy)32+) and aminoglycoside antibiotics(e.g., gentamycin, streptomycin, dihydrostreptomycin, Lincomycin with secondary or Tertiary amine in molecule structure). After adding CNPs, the detection limit is 0.0347, 0.0350, 0.1187, 0.0169 μM (S/N = 3) for gentamycin, streptomycin, dihydrostreptomycin and lincomycin respectively, were obtained under the optimized conditions. Before adding CNPs the detection limit is 0.0732, 0.3186, 0.2308, 0.0668 μM (S/N = 3) for gentamycin, dihydrostreptomycin, streptomycin and lincomycin respectively. It represents CNPs can be a electrocatalyst in ECL mechanism with (Ru(bpy)32+) and aminoglycoside antibiotics. The developed method was successfully applied to the determination of gentamycin and Lincomycin in real samples with recoveries in the range of 98.25?103.50% (n = 3).en_US
dc.description.abstract本篇論文主要分成兩部份,首先是研究以電化學方式製備碳奈米材料(carbon nanoparticles, CNPs)的電化學發光(Electrogenerated chemiluminescence , ECL)性質,第二部份則為CNPs搭配在Ru(bpy)32+的ECL系統上的應用。 研究結果發現CNPs與大多數的奈米碳材(MWCNT﹑Graphene oxide)一樣擁有ECL性質,以循環伏安法在電位0.4至2.3V之間掃描,可觀察到有產生ECL訊號,參考文獻推測其ECL反應機構主要為碳材表面的羥基及醛基在高電位下被氧化產生自由基後,再經過自由基電子轉移生成激發態物種後放光所測得,其後將把CNPs加入在Ru(bpy)32+的ECL系統中,藉由CNPs在弱鹼溶液中所帶負電荷能將帶正電荷的Ru(bpy)32+吸附在其表面,再加上高氧化電位時CNPs表面羥基能產生自由基,所以能提高Ru(bpy)32+所產生出的ECL反應訊號。 在以CNPs- Ru(bpy)32+之ECL系統偵測抗生素應用上,結果顯示CNPs能使Ru(bpy)32+偵測Gentamycin、Streptomycin、Dihydrostreptomycin、Lincomycin四種抗生素的LOD值下降,並成功實際應用於抗生素藥品的偵測。zh_TW
dc.description.tableofcontents摘要 I Abstract II 目錄 IV 圖目錄 VI 表目錄 IX 第一章 緒論 1 第一節 文獻回顧 1 第二節 碳奈米材料(Carbon nanoparticles, CNPs)性質簡介 12 第三節 電化學分析法簡介 17 3-1 三電極系統 17 3-2 循環伏安法(cyclic voltammetry, CV) 19 3-3 安培法(Amperometry, i-t curve) 20 3-4 流動注入分析法(Flow injection analysis, FIA) 22 第二章 儀器與藥品 24 第一節 儀器設備 24 第二節 藥品目錄與配製方法 26 2-1 藥品目錄 26 2-2 藥品配製 28 第三節 實驗裝置 32 第三章 CNPs之ECL性質探討 34 第一節 CNPs的螢光與ECL性質探討 35 3-1-1 以UV-vis與螢光光譜觀察CNPs 35 3-1-2 CNPs的TEM粒徑觀察結果 39 3-1-3 CNPs的Ru(bpy)32+ECL反應催化效果探討 40 3-1-4 CNPs的ECL反應機制探討 48 第二節 結論 58 第四章 控制溫度預氧化網版印刷碳電極在Ru(bpy)32+電化學發光系統研究 59 第一節 控制溫度與預氧化時間的Ru(bpy)32+-ECL測試 60 第二節 結論 67 第五章 Ru(bpy)32+-CNPs的ECL系統在偵測抗生素藥品的應用 68 第一節 胺基配醣體抗生素的電化學行為與ECL反應機制探討 71 第二節 CNPs-Aminoglycoside- Ru(bpy)32+ECL系統最佳化 80 5-2-1 系統最佳pH值探討 80 5-2-2 CNPs稀釋比例與混合比例對偵測抗生素的效果探討 82 5-2-3 系統掃描速率及最佳流速探討 84 第三節 檢量線之建立與系統穩定性測試 86 5-3-1 CNPs-Aminoglycoside- Ru(bpy)32+系統建立抗生素檢量線 86 5-3-2 CNPs-Aminoglycoside- Ru(bpy)32+系統穩定性測試 90 第四節 抗生素藥物真實樣品測試 92 第五節 結論 97 第六章 未來展望 98 第一節 CNPs修飾奈米Cu粒子在偵測神經傳導物質的應用 98zh_TW
dc.language.isozh_TWzh_TW
dc.rights同意授權瀏覽/列印電子全文服務,2017-06-29起公開。zh_TW
dc.subjectCarbon nanoparticleen_US
dc.subjectScreen-printed carbon electrodeen_US
dc.subjectIndium tin oxideelectrodeen_US
dc.subjecttris(2,2'-bipyridyl)ruthenium(II)en_US
dc.subjectaminoglycoside antibioticsen_US
dc.subjectElectrogeneratedChemiluminescenceen_US
dc.subject奈米碳材zh_TW
dc.subject網版印刷碳電極zh_TW
dc.subject胺基配醣體抗生素zh_TW
dc.subject電化學發光法zh_TW
dc.titleThe development and application of carbon nanoparticle in Electrogenerated Chemiluminescence (ECL)en_US
dc.title電合成奈米碳材之電化學放光性質探討與應用zh_TW
dc.typeThesis and Dissertationen_US
dc.date.paperformatopenaccess2017-06-29zh_TW
dc.date.openaccess2017-06-29-
item.grantfulltextrestricted-
item.openairetypeThesis and Dissertation-
item.languageiso639-1zh_TW-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
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