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標題: Rapid Analysis and Quantification of Polyphenol Compounds Using Graphene-based Sponge
Rapid Analysis and Quantification of Polyphenol Compounds Using Graphene-based Sponge
作者: 賴柔伶
Jo-Lene Lai
關鍵字: 多酚類化合物;石墨烯海綿;快速分析;polyphenol compounds;MALDI;graphene-based sponge
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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been applied on wide range of biomolecules analysis based on its advantage of speed of analysis, sensitive, ease of use and data interpretation. However, MALDI contains limitation on matrix interference at low mass region and sweet spot searching, thus leading to the development of surface assisted laser desorption/ionization (SALDI). SALDI uses inorganic matrices which can reduce the matrix interference and capable in analyzing small molecules. Graphene is widely used in SALDI approaches and aid in analyzing small molecules such as fatty acids, amino acids and flavonoids. According to previous research, strong van der Waals interaction is formed between graphene nanosheet and 3M sponge thus created graphene-coated sponge. This graphene-coated sponge is used as a versatile and recyclable sorbent material. In this experiment, various tests such as graphene concentration optimization, evaluation of graphene flakes analysis on polyphenol compounds, dip coating solutions and time in the making of graphene-based sponges and others were proceeded to develop a rapid analyse and quantification polyphenol compounds platform. Results showed that graphene flakes are the best matrix in polyphenol compounds analysis when compare to LDI analysis and conventional methods using CHCA and DHB as matrices. Furthermore, graphene-based sponges made with graphene concentrations of 0.05 mg/mL coupled with 25s of coating time and coating solution of 50% ethanol yielded the highest sensitivity and similar results with the analysis using graphene flakes. By using this platform, the 3 stable polyphenol compounds (morin hydrate, quercitrin hydrate and quercetin-3-β-Dglucoside) were successfully quantified. In conclusion, the rapid analysis and quantification polyphenol compounds platform using graphene-based sponges was successfully developed.

基質輔助雷射脫附游離飛行時間式質譜儀 (Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF MS) 具快速和高靈敏度特性,常應用於分析蛋白質、胜肽和小分子等。然而,傳統分析方法中分析物與有機基質相互混合,易產生非均勻共結晶,進而導致訊號集中點 (sweet spot),並造成分子量小於 500 Da之基質訊號干擾,影響小分子分析。因此,為解決小分子分析困難,Sunner等人於1995年發展出表面輔助雷射脫附游離法 (Surface-assisted laser desorption/ionization, SALDI)。SALDI 利用無極基質進行分析,降低分子量500 Da以下訊號干擾,有利於小分子分析,改善MALDI缺點。前人研究顯示,石墨烯廣泛應用於脂肪酸、胺基酸和類黃酮等小分子分析,並可透過與海綿間的π-π相互作用,形成石墨烯海綿。因此,本篇研究利用石墨烯海綿建立快速分析及定量酚類化合物之平台。本次實驗首先測試石墨烯濃度對於酚類化合物分析之最佳化條件,接著利用石墨烯最佳化濃度 (0.05 mg/mL) 搭配不同濃度 (0%、50%、100%) 之乙醇及不同震盪時間 (5、10、15、20、25和30秒)以測試石墨烯海綿最佳化製作並建立快速分析及定量平台
。實驗結果顯示,利用含有石墨烯濃度0.05 mg/mL 之50% 乙醇搭配25秒震盪時間製作石墨烯海綿,成功分析10種酚類化合物,其結果趨近於單純利用石墨烯分析方法,並成功將3種酚類化合物 (morin hydrate, quercitrin hydrate, quercetin-3-β-D-glucoside) 進行定量分析,未來可作為酚類化合物產品之快速量化平台。
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