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http://hdl.handle.net/11455/92266
標題: | Study of rapid cell death and pathogenesis of Xanthomonas axonopodis pv. glycines Xanthomonas axonopodis pv. glycines 細胞凋亡機制與病原性基因之探討 |
作者: | 蔡宗翰 Chung-Han Tsai |
關鍵字: | 葉燒病;黑腐菌;大豆;快速死亡;black rot;Xanthomonas;soy bean;rapid cell death;RCD | 引用: | Baumler, A.J., Norris, T.L., Lasco, T., Voight, W., Reissbrodt, R., Rabsch, W., and Heffron, F. (1998). IroN, a novel outer membrane siderophore receptor characteristic of Salmonella enterica. J Bacteriol 180, 1446-1453. Davalos-Garcia, M., Conter, A., Toesca, I., Gutierrez, C., and Cam, K. (2001). Regulation of osmC gene expression by the two-component system rcsB-rcsC in Escherichia coli. J Bacteriol 183, 5870-5876. Engelberg-Kulka, H., Amitai, S., Kolodkin-Gal, I., and Hazan, R. (2006). Bacterial programmed cell death and multicellular behavior in bacteria. PLoS Genet 2, e135. Gautam, S., and Sharma, A. (2002a). Involvement of caspase-3-like protein in rapid cell death of Xanthomonas. Mol Microbiol 44, 393-401. Gautam, S., and Sharma, A. (2002b). Rapid cell death in Xanthomonas campestris pv. glycines. J Gen Appl Microbiol 48, 67-76. He, Y.W., Ng, A.Y., Xu, M., Lin, K., Wang, L.H., Dong, Y.H., and Zhang, L.H. (2007). Xanthomonas campestris cell-cell communication involves a putative nucleotide receptor protein Clp and a hierarchical signalling network. Mol Microbiol 64, 281-292. Hwang, I., Lim, S.M., and Shaw, P.D. (1992a). Use of detached soybean cotyledons for testing pathogenicity of Xanthomonas camprestris pv. glycines. Plant Dis 76, 182-183. Hwang, I.Y., Lim, S.M., and Shaw, P.D. (1992b). Use of Detached Soybean Cotyledons for Testing Pathogenicity of Xanthomonas-Campestris Pv Glycines. Plant Dis 76, 182-183. Iwase, T., Tajima, A., Sugimoto, S., Okuda, K., Hironaka, I., Kamata, Y., Takada, K., and Mizunoe, Y. (2013). A simple assay for measuring catalase activity: a visual approach. Sci Rep 3, 3081. Jeffrey, V., and Joachim, M. (1991). New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Gene 100, 189-194. Kawamukai, M., Kishimoto, J., Utsumi, R., Himeno, M., Komano, T., and Aiba, H. (1985). Negative regulation of adenylate cyclase gene (cya) expression by cyclic AMP-cyclic AMP receptor protein in Escherichia coli: studies with cya-lac protein and operon fusion plasmids. J Bacteriol 164, 872-877. Locher, K.P., Rees, B., Koebnik, R., Mitschler, A., Moulinier, L., Rosenbusch, J.P., and Moras, D. (1998). Transmembrane signaling across the ligand-gated FhuA receptor: crystal structures of free and ferrichrome-bound states reveal allosteric changes. Cell 95, 771-778. Lusetti, S.L., Wood, E.A., Fleming, C.D., Modica, M.J., Korth, J., Abbott, L., Dwyer, D.W., Roca, A.I., Inman, R.B., and Cox, M.M. (2003). C-terminal deletions of the Escherichia coli RecA protein. Characterization of in vivo and in vitro effects. J Biol Chem 278, 16372-16380. Mehdy, M.C. (1994). Active Oxygen Species in Plant Defense against Pathogens. Plant Physiol 105, 467-472. Pham, H.T., Riu, K.Z., Jang, K.M., Cho, S.K., and Cho, M. (2004). Bactericidal activity of glycinecin A, a bacteriocin derived from Xanthomonas campestris pv. glycines, on phytopathogenic Xanthomonas campestris pv. vesicatoria cells. Appl Environ Microbiol 70, 4486-4490. Qian, W., Jia, Y., Ren, S.X., He, Y.Q., Feng, J.X., Lu, L.F., Sun, Q., Ying, G., Tang, D.J., Tang, H., et al. (2005). Comparative and functional genomic analyses of the pathogenicity of phytopathogen Xanthomonas campestris pv. campestris. Genome Res 15, 757-767. Raju, K.K., Gautam, S., and Sharma, A. (2006). Molecules involved in the modulation of rapid cell death in Xanthomonas. J Bacteriol 188, 5408-5416. Raju, K.K., Misra, H.S., and Sharma, A. (2007). Xanthomonas caspase displays an inherent PARP-like activity. FEMS Microbiol Lett 272, 259-268. Rukayadi, Y., Suwanto, A., Tjahjono, B., and Harling, R. (2000). Survival and epiphytic fitness of a nonpathogenic mutant of Xanthomonas campestris pv. glycines. Appl Environ Microbiol 66, 1183-1189. Schweizer, H.D. (1993). Small broad-host-range gentamycin resistance gene cassettes for site-specific insertion and deletion mutagenesis. Biotechniques 15, 831-834. Soldani, C., Lazze, M.C., Bottone, M.G., Tognon, G., Biggiogera, M., Pellicciari, C.E., and Scovassi, A.I. (2001). Poly(ADP-ribose) polymerase cleavage during apoptosis: when and where? Exp Cell Res 269, 193-201. Thornberry, N.A., and Lazebnik, Y. (1998). Caspases: Enemies within. Science 281, 1312-1316. Vauterin, L., Hoste, B., Kersters, K., and Swings, J. (1995). Reclassification of xanthomonas. International Journal of Systematic Bacteriology 45, 472-489. Wadhawan, S., Gautam, S., and Sharma, A. (2010). Metabolic stress-induced programmed cell death in Xanthomonas. FEMS Microbiol Lett 312, 176-183. Watson, R.J., Millichap, P., Joyce, S.A., Reynolds, S., and Clarke, D.J. (2010). The role of iron uptake in pathogenicity and symbiosis in Photorhabdus luminescens TT01. BMC Microbiol 10, 177. Yang, B.Y., and Tseng, Y.H. (1988). Production of exopolysaccharide and levels of protease and pectinase activity in pathogenic and non-pathogenic strains of Xanthomonas campestris pv. campestris. Bot Bull Acad Sinica 29, 93-99. 張哲杰 (2014). Xanthomonas campestris pv. glycines 細胞快速死亡現象之探討. In Institute of Molecular Biology (National Chung Hsing University). 陳義元 (2011). Xanthomonas campestris pv. campestris 與 Stenotrophomonas maltophilia 的主要外膜蛋白 MopB 之特性探討 (National Chung Hsing University). | 摘要: | Many eukaryotic organisms practice programmed cell death (PCD) to eliminate damaged or abandoned cells. Recently, some studies suggest that some prokaryotes also possess PCD mechanism. For an example, Xanthomonas axonopodis pv. glycines (Xag) undergoes rapid cell death (RCD) when grown in LB medium for 48 hr but not in starch medium. In order to study the mechanism of RCD, Xag and the RCD-deficient Xanthomonas campestris pv. campestris (Xcc) were compared and no significant differences in growth and survival rates were observed. Using random transposon EZ-Tn5 for mutagenesis, three Xag mutants that initially showed less or no RCD were isolated. However, consistent RCD was no longer observed in further experiments. In LB medium, FeSO4 at low concentration was found to slow down RCD of Xcg, while the cell growth was inhibited at high concentrations. This is in agreement with the result of a previous study showing that FeSO4 can reduce RCD. To further study the Xcg RCD, several mutants were isolated based on the microarray data. Analysis on these mutants revealed that 1) XagcatB, a catalase mutant, has a catalase level which was 50% of that in wild type, but no significant change in RCD was observed, 2) growth of XagiroN, XagfhuA, Xc17iroN, and Xc17fhuA was not affected by iron deficiency in the medium, although it has been shown that FhuA and IroN play some roles in ferric uptake in Xag and Xc, 3) levels of extracellular enzymes were not altered in mutants catB, fhuA, and iroN,and 4) Xag mutants catB, mopB, and fhuA exhibited reduced virulence in causing pustule disease in soybean. 真核生物利用細胞計畫性死亡 (programmed cell death, PCD) 機制控制細胞自殺與消滅受損或不需要的細胞。近年來發現在原核生物中亦有 PCD 機制,例如 Xanthomonas axonopodis pv. glycines (Xag) 的 rapid cell death (RCD) 現象。先前研究發現將 Xag 培養在 LB medium 中48小時後會有 RCD 現象,而在 starch medium 中則否。為探討造成 Xanthomonas RCD 現象之機制,本研究中首先比較 Xag 與 Xanthomonas campestris pv. campestris (Xcc) 在不同培養時期之生長曲線與存活率。Xag 有出現RCD 現象,Xc17則無。然後利用 EZ-Tn5™ |
URI: | http://hdl.handle.net/11455/92266 | Rights: | 同意授權瀏覽/列印電子全文服務,2018-08-28起公開。 |
Appears in Collections: | 分子生物學研究所 |
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