Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/92608
標題: 易中風型自發性高血壓大鼠的血液生化分析及組織病理特徵之研究
Characterization of Serum Biochemistry and Histopathology in Stroke-prone Spontaneous Hypertension Rat
作者: 李佳紋
Chia-wen Lee
關鍵字: 高血壓;中風;步態;易中風型自發性高血壓大鼠;hypertension;stroke;sciatic function index;stroke prone spontaneously hypertensive rats
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摘要: 
高血壓每年導致全球710 萬人死亡,占全部死亡人口13%。高血壓患
者因長期動脈壓持續性升高進而導致許多併發症,常見的有冠心病、糖尿
病、心力衰竭、高血脂、腎病、周圍動脈疾病、中風、心室肥厚等,這些
造成龐大的社會醫療資源負擔。在已開發和開發中國家中,出血性中風更
是造成長期殘疾或死亡主要原因之一,未來十年人口老化及生活習慣不佳
的情況下,此影響勢必越來越嚴重。本研究試驗使用動物模式為易中風型
自發性高血壓大鼠(SHRSP),其本身帶有高血壓相關併發症且容易因小血
管問題而發生腦中風之情況,進而造成全身性疾病問題,實驗以50-60 週
齡WKY 當作對照組;另外使用30、50、70 週齡之雄鼠;20-35、35-45、
50-65、80-95 週齡之雌鼠;已中風之SHRSP 大鼠,進行血液生化分析、臟
器重量分析、步態紀錄、腦部病理切片分析及腎臟組織病理型態變化分析
等。試驗結果發現,雄鼠於脂質相關檢測隨年齡增長有升高趨勢,顯示隨
著年齡增加,體內也堆積更多的脂質,而雌鼠於脂質相關檢測則無顯示相
同結果,反而在麩草醋酸轉胺酶(AST)及與血中尿素氮(BUN)有上升趨勢。
犧牲後臟器重量分析發現自發性易中風型高血壓公鼠之腦、腎臟、肝臟與
腎上腺重量佔比與WKY 公鼠相比有明顯增加。易中風型自發性高血壓雌
鼠方面,在腎臟與腎上腺重量佔比與WKY 雌鼠相比有明顯增加,由於易
中風型自發性高血壓大鼠主要病變區域發生在腦、心臟、及腎臟。由此臟
器重量佔比亦可發現其相關程度。在步態測試方面,中風之SHRSP 之步
態行為嚴重受到影響而與未中風之對照組有明顯之差異。而在大鼠腦部
TTC 染色可以看出,中風大鼠腦部出現缺血情況。組織病理H&E 染色可
明顯比較出已經中風之SHRSP 大鼠因長期處於高血壓狀態下而引發慢性
腎臟病變。期望藉由本實驗能更了解中風病理生理的複雜性變化,進一步
可應用於其他研究,找出更有效預防高血壓或發展新式藥物之治療方法。

Hypertension causes 7.1 million deaths worldwide every year, accounts
for 13% of all deaths. The patients with hypertension due to long-term arterial
pressure that leads to many complications, including the coronary heart
disease, diabetes, the heart failure, high cholesterol, kidney disease,
peripheral arterial disease, stroke and ventricular hypertrophy, which cause
huge waste of social medical resource. In the developed and developing
countries, hemorrhagic stroke was one of the main to cause long-term
disability and death. In the next decade, the aging population and poor living
habits are getting worse. In this study, stroke prone spontaneously
hypertensive rats with hypertension-related complications are imitated
cerebral apoplexy. That finally cause the body-wide diseases. The rats were
assigned to three group: (1) Normal control group (50 and 60 week old WKY
rats) (2) Cerebral apoplexy of male rats (30, 50 and 70 weeks) (3) Cerebral
apoplexy of female rats (20-35, 35-45, 50-65 and 80-95 weeks). The
biochemical analysis, the organs weights, the gait record, brain pathological
section and kidney histopathology analyses of the SHRSP rats with stroke
were recorded . The results showed that with the increasing of age, the male
rats increased the lipid accumulation, but no females showed the same results
in the lipid accumulation. In addition, the bran oxaloacetate transglutaminase
enzymes and blood urea nitrogen of female rats increased with the increasing
of age. After sacrificed, brain of male SHRSP rats, kidney, liver and adrenal
weight was significantly increased compared to the WKY male rats. Female
SHRSP significantly attenuates the kidney and adrenal weight compared with
WKY females gain, mainly due to the lesions of SHRSP rats occur in the brain,
heart, and kidneys. In gait testing, gait behavior of SHRSP with stroke was
obviously different with the control group. TTC staining in rat brain showed
that there is ischemic stroke cases in rat brain. H & E histopathological show
that the stroke of SHRSP rats in a long period of hypertension lead to chronic
kidney disease. In this study, we expect a better understanding of the
complexity of the pathophysiology of stroke changes, and in the further it can
be applied to other research to identify more effective in preventing the
development of hypertension or treatment of new medicine.
URI: http://hdl.handle.net/11455/92608
其他識別: U0005-0302201516114800
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