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標題: 無患子皂苷萃取物對非小細胞肺癌株A549的抗癌活性分析
Anticancer activity of the extracts of Sapindus saponaria against Non-small-cell lung carcinoma A549 cell line
作者: 賴蕙琪
Hui-Chi Lai
關鍵字: 無患子;皂苷;肺癌;sapindus saponaria;saponins;lung cancer
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無患子果實中含高濃度的皂苷成份,而皂苷具有抗菌抗癌的效果,因此本研究以無患子多種甲醇分層萃取物利用MTT assay進行不同種癌細胞的細胞毒性測試。結果和前之實驗相符,甲醇萃取物 (ESS1)對非小細胞肺癌細胞A549毒殺效果最佳。另外將ESS1使用ethyl acetate溶劑和水進行分層,分別獲得ESS2和ESS3。將其中水層ESS3使用KOH水解後再利用ethyl acetate萃取,獲得ESS4,再使用MTT assay進行不同無患子皂苷萃取物對A549細胞株的細胞毒性測試,確定ESS2效果最佳。藉由流式細胞儀分析,ESS2能使A549細胞株的細胞週期停留在G1期,進一步使用雙染方式,證實ESS2能誘導A549細胞株產生細胞壞死。另外將會誘導細胞壞死的ESS1各別與會誘導細胞壞死的玫瑰天竺葵精油或與會誘導細胞凋亡的奧勒岡精油混合使用,利用MTT assay對非小細胞肺癌細胞A549進行細胞毒性測試,再藉由Chou-Talalay公式計算其CI值,證明ESS1與這兩種精油無法產生協同作用。

The fruit of Sapindus saponaria contains high concentration of saponin with antibacterial and anticancer effects. The aim of this study is to apply the various separations of methanol extract of S.saponaria on different kinds of cancer cells to examine their anticancer activity with MTT assay. The result showed that non-small cell lung carcinoma A549 cell is the most susceptible one for the cytotoxicity of the methanol extract of S.saponaria (ESS1), same as that of the previous study. The ESS1 was then fractioned into ethyl acetate layer (ESS2) and water layer (ESS3). The water fraction, ESS3, of ESS1 was hydrolyzed with KOH followed by the re-extraction of ethyl acetate to obtain ESS4. MTT assay was used again to determine the cytotoxicity on A549 cells with different kinds of extraction layers of S. saponaria. The data appeared that ESS2 showed the best result for cytotoxicity test. Through the flow cytometry analysis, ESS2 can arrest A549 cell growth at G1 phase of the cell cycle. The data of double staining experiment showed that ESS2 induced A549 cell to cell death with the necrosis pathway, same as that of ESS1. ESS1was combined formulation with the essential oil extract of Pelargonium capitatum, induced cell necrosis, or with the essential oil extract of Origanum vulgare, induced cell apoptosis, to A549 cells for synergic effect of treatments. After the MTT assay performance and through the Chou-Talalay equation to obtain CI values, both of the combined formulations observe no synergy effects.
其他識別: U0005-2701201401360200
Rights: 不同意授權瀏覽/列印電子全文服務
Appears in Collections:生命科學院碩士在職專班

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