Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/92619
標題: 素食食品攙動物性成分調查
Survey of Animal-Derived Ingredients Adulteration in Vegetarian Foods
作者: 呂俊賢
Chun-Hsien Lu
關鍵字: 素食食品;動物性成分;聚合酶連鎖反應;酵素連結免疫吸附法;vegetarian food;animal-derived;PCR;ELISA
引用: 參考文獻 行政院衛生福利部食品藥物管理署(2008)。包裝食品宣稱為素食之標示規定。97 年7月1日衛署食字第0970402575號公告。行政院衛生福利部食品藥物管理 署,台北市。 行政院衛生福利部食品藥物管理署(2013a)。食品中動物性成分檢驗方法-定性 篩選檢驗102年11月26日部授食字第1021951033 號公告修正。行政院衛生 福利部食品藥物管理署,台北市。 行政院衛生福利部食品藥物管理署(2013b)。食品中動物性成分檢驗方法-牛成 分之定性檢驗,102年11月27日部授食字第1021951074 號公告修正。行政院衛 生福利部食品藥物管理署,台北市。 行政院衛生福利部食品藥物管理署(2013c)。食品中動物性成分檢驗方法-雞成 分之定性檢驗,102年11月27日部授食字第1021951087 號公告修正。行政院衛 生福利部食品藥物管理署,台北市。 行政院衛生福利部食品藥物管理署(2013d)。食品中動物性成分檢驗方法-猪成 分之定性檢驗,102年11月27日部授食字第1021951081 號公告修正。行政院衛 生福利部食品藥物管理署,台北市。 行政院衛生福利部食品藥物管理署(2014)。公告資訊:食藥署以分子生物技術檢 驗讓素食攙葷無所遁形,發布日期:08-21。行政院衛生福利部食品藥物管理署, 台北市。取自 http://www.fda.gov.tw/TC/newsContent.aspx?id=11452&chk=3e3fc615-fe9d-4aa4-9cfb-0ff14e77e337#.VQvLJY6Uffi. 林書賢(2005)。市售組織化植物蛋白(Texturized Vegetable Protein ,TVP)物理特性與感官品質間之相關性及其最適品質判定之探討。國立臺灣海洋大學食品科學系碩士論文。基隆。 洪維新、李瑞玲、劉名榜(1998)。添加非肉蛋白質對重組豬排品質之影響。台糖畜產,3:59-65。 姚富洲(2010)。新編生物技術。合記圖書出版社。台北。 崔秀煒、張源鑫、簡子齡、林澤揚、林旭陽、闕麗卿(2011)。99年市售素食食品攙動物性成分調查。衛生福利部食品藥物食品藥物管理署。食品藥物研究年報. 2 : 214-222。 張勝善(1994)。蛋品加工學。華香園出版社。台北。 張育彰、黃郁珺、黃維生、陳瑜絢、李明鑫、陳惠芳、施養志、潘志寬、蔡 淑貞(2014)。102年度食品後市場監測結果與統計分析。衛生福利部食品藥物食品藥物管理署。食品藥物研究年報. 5 : 334-342。 鄭穎勵(2007)。含大豆蛋白蒟蒻製品之探討。中國文化大學生活應用科學研究所碩士論文。台北。 Alves R.C., Barroso M.F., González-García MB, Oliveira MB, and Delerue-Matos MC. (2015). New Trends in Food Allergens Detection: Towards Biosensing Strategies.Crit Rev Food Sci Nutr, 0. Abbott, M., Hayward, S., Ross, W., Godefroy, S. B., Ulberth, F., Van Hengel, A. J., et al. (2010). Validation procedures for quantitative food allergen ELISA methods: Community guidance and best practices. Journal of AOAC International, 93(2): 442–450. Calvo, J. H., Zaragoza, P., and Osta, R. (2001). Random amplified olymorphic DNA fingerprints for identification of species in poultry pate. Poult. Sci ,80: 522-524. Hirose, M., H. Oe and E. Doi. (1986). Thiol-dependent gelation of egg white. Agric. Biol. Chem , 50: 59-64. Parris, N. and Baginski, M. A. (1991). A rapid method for determination of whey protein denaturation. J. Dairy Sci , 74: 58-64.
摘要: 
素食中含有動物性成分在歷年調查中皆可發現,其中尤以散裝產品為多,由於傳統動物性成份使用PCR技術檢測,而PCR 檢測需要樣品中含有 DNA成份,才可進行成份鑑別。蛋白(粉)成份中沒有 DNA 成份故無法使用 PCR 技術檢測,成為檢測盲點,本研究除瞭解市售散裝素食產品動物性成分的添加比例亦擬了解以ELISA技術辨識素食添加蛋白粉之可能性。實驗於素食專賣店與傳統市場抽樣散裝素食產品63件,先以PCR技術測動物性成分比例,判定為全素者再以ELISA 辨識有無添加蛋白粉,結果發現,經PCR 檢測有46件(73%)產品符合素食規定。17件(27%)不符合規定產品,2件檢驗出豬成份,6件檢驗出魚成份,2件同時驗出雞及豬成份,2件同時驗出含有雞及魚成份,1件產品檢驗出含有魚及豬成份,4件產品檢驗出同時含有雞、魚及豬成份。將27件動物性成分零檢出產品進一步使用Egg White ELISA kit進行蛋白成分鑑別,發現有18件(67%)產品含有蛋白成份。研究結果顯示ELISA 技術可成功辨識蛋白(粉)成份,故在現行的素食食品動物性成份鑑別方法建議可增加 ELISA 檢測項目,使檢測結果可更全面。

From the recently survey found that vegetarian food contain animal-derived material, especially in unpackaged. The traditional assay is using PCR (Polymerase Chain Reaction) technology to determine the animal-derived material which only can detected the samples contained DNA. Therefore, some animal-derived material like egg white (powder) which doesn't contained any DNA will be limited by the PCR assay, become blind spot. This study aims to determine the animal-derived material added ratio in unpackaged vegetarian food sold in traditional market. Based on previous description, this study also going to estimate the possibility of using ELISA (Enzyme-linked immunosorbent assay) assay to detect the egg white (powder) added in the product. 63 unpackaged vegetarian products were sampled from traditional market and stores. The animal-derived material ratio by PCR assay was determined first, after that the vegan were then determined the ratio of egg white (powder) added product by Egg White ELISA kit. The results indicated that 46 products (73%) compliance the vegan rule.17 products (27%) contained animal-derived material. For the 17 products, there are 2 products contained pig derived material, 6 products contained fish derived material, 2 products contained chicken and pig derived material, 2 products contained chicken and fish derived material,1 product contained fish and pig derived material and 4 products contained pig,fish and chicken derived material. 27 products from 46 vegan foods were then determined the egg white (powder) by Egg White ELISA assay. The result found the egg white (powder) were found in 18 product (67%) of the 27 vegan foods. According to our results, it will be more complete to add ELISA assay into vegetarian food containing animal-derived material testing method system of the standard method of the goverment.
URI: http://hdl.handle.net/11455/92619
其他識別: U0005-1208201510553800
Rights: 同意授權瀏覽/列印電子全文服務,2017-08-13起公開。
Appears in Collections:生命科學院碩士在職專班

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