Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/92623
標題: 評估基質輔助雷射脫附離子化-飛行時間質譜儀分型近平滑念珠菌群之可行性
Evaluation of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry for differentiation of Candida parapsilosis complex
作者: 曾耀嬬
Yao-Ru Tseng
關鍵字: 近平滑念珠菌群;基質輔助雷射脫附離子化-飛行時間質譜儀;外顯子間填裝內含子之聚合酶連鎖反應;Candida parapsilosis complex;Matrix-assisted laser desorption / ionization time-of-flight mass spectrometry;Exon-primed intron-crossing polymerase chain reaction
引用: 1. Almirante, B., D. Rodriguez, M. Cuenca-Estrella, M. Almela, F. Sanchez, J. Ayats, C. Alonso-Tarres, J. L. Rodriguez-Tudela and A. Pahissa (2006). Epidemiology, risk factors, and prognosis of Candida parapsilosis bloodstream infections: case-control population-based surveillance study of patients in Barcelona, Spain, from 2002 to 2003. J Clin Microbiol 44: 1681-1685. 2. Baillie, G. S. and L. J. Douglas (1999). Role of dimorphism in the development of Candida albicans biofilms. J Med Microbiol 48: 671-679. 3. Barbuddhe, S. B., T. Maier, G. Schwarz, M. Kostrzewa, H. Hof, E. Domann, T. Chakraborty and T. Hain (2008). Rapid identification and typing of listeria species by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Appl Environ Microbiol 74: 5402-5407. 4. Bertini, A., F. De Bernardis, L. A. Hensgens, S. Sandini, S. Senesi and A. Tavanti (2013).Comparison of Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis adhesive properties and pathogenicity. Int J Med Microbiol 303: 98-103. 5. Bonassoli, L. A., M. Bertoli and T. I. Svidzinski (2005).High frequency of Candida parapsilosis on the hands of healthy hosts. J Hosp Infect 59: 159-162. 6. Brockerhoff, H. (1974).Model of interaction of polar lipids, cholesterol, and proteins in biological membranes.Lipids 9: 645-650. 7. Cendejas-Bueno, E., A. Gomez-Lopez, E. Mellado, J. L. Rodriguez-Tudela and M. Cuenca-Estrella (2010). Identification of pathogenic rare yeast species in clinical samples: comparison between phenotypical and molecular methods. J Clin Microbiol 48: 1895-1899. 8. Chen, Y. C., Y. H. Lin, K. W. Chen, J. Lii, H. J. Teng and S. Y. Li (2010). Molecular epidemiology and antifungal susceptibility of Candida parapsilosis sensu stricto, Candida orthopsilosis, and Candida metapsilosis in Taiwan. Diagn Microbiol Infect Dis 68: 284-292. 9. Clark, T. A., S. A. Slavinski, J. Morgan, T. Lott, B. A. Arthington-Skaggs, M. E. Brandt, R. M. Webb, M. Currier, R. H. Flowers, S. K. Fridkin and R. A. Hajjeh (2004). Epidemiologic and molecular characterization of an outbreak of Candida parapsilosis bloodstream infections in a community hospital. J Clin Microbiol 42: 4468-4472. 10. Clerihew, L., T. L. Lamagni, P. Brocklehurst and W. McGuire (2007). Candida parapsilosis infection in very low birthweight infants. Arch Dis Child Fetal Neonatal Ed 92: F127-129. 11. Contreras, I., J. Ponton and G. Quindos (1994). Prevalence of Candida parapsilosis in the oral cavities of infants in Spain. Clin Infect Dis 18: 480-481. 12. Davey, K. G., P. M. Chant, C. S. Downer, C. K. Campbell and D. W. Warnock (1995).Evaluation of the AUXACOLOR system, a new method of clinical yeast identification. J Clin Pathol 48: 807-809. 13. Dhiman, N., L. Hall, S. L. Wohlfiel, S. P. Buckwalter and N. L. Wengenack (2011). Performance and cost analysis of matrix-assisted laser desorption ionization-time of flight mass spectrometry for routine identification of yeast. J Clin Microbiol 49: 1614-1616. 14. Fridkin, S. K., D. Kaufman, J. R. Edwards, S. Shetty and T. Horan (2006). Changing incidence of Candida bloodstream infections among NICU patients in the United States: 1995-2004. Pediatrics 117: 1680-1687. 15. Gomez-Lopez, A., A. Alastruey-Izquierdo, D. Rodriguez, B. Almirante, A. Pahissa, J. L. Rodriguez-Tudela and M. Cuenca-Estrella (2008). Prevalence and susceptibility profile of Candida metapsilosis and Candida orthopsilosis: results from population-based surveillance of candidemia in Spain. Antimicrob Agents Chemother 52: 1506-1509. 16. Goncalves, S. S., C. S. Amorim, M. Nucci, A. C. Padovan, M. R. Briones, A. S. Melo and A. L. Colombo (2010). Prevalence rates and antifungal susceptibility profiles of the Candida parapsilosis species complex: results from a nationwide surveillance of candidaemia in Brazil. Clin Microbiol Infect 16: 885-887. 17. Huang, Y. C., T. Y. Lin, H. S. Leu, H. L. Peng, J. H. Wu and H. Y. Chang (1999).Outbreak of Candida parapsilosis fungemia in neonatal intensive care units: clinical implications and genotyping analysis. Infection 27: 97-102. 18. Kantarcioglu, A. S. and A. Yucel (2002).Phospholipase and protease activities in clinical Candida isolates with reference to the sources of strains. Mycoses 45: 160-165. 19. Kim, S. K., K. El Bissati and C. Ben Mamoun (2006).Amino acids mediate colony and cell differentiation in the fungal pathogen Candida parapsilosis. Microbiology 152: 2885-2894. 20. Kuhn, D. M., J. Chandra, P. K. Mukherjee and M. A. Ghannoum (2002). Comparison of biofilms formed by Candida albicans and Candida parapsilosis on bioprosthetic surfaces. Infect Immun 70: 878-888. 21. Kuhn, D. M., T. George, J. Chandra, P. K. Mukherjee and M. A. Ghannoum (2002). Antifungal susceptibility of Candida biofilms: unique efficacy of amphotericin B lipid formulations and echinocandins. Antimicrob Agents Chemother 46: 1773-1780. 22. Laffey, S. F. and G. Butler (2005).Phenotype switching affects biofilm formation by Candida parapsilosis.Microbiology 151: 1073-1081. 23. Levy, I., L. G. Rubin, S. Vasishtha, V. Tucci and S. K. Sood (1998). Emergence of Candida parapsilosis as the predominant species causing candidemia in children. Clin Infect Dis 26: 1086-1088. 24. Lockhart, S. R., S. A. Messer, M. A. Pfaller and D. J. Diekema (2008). Lodderomyces elongisporus masquerading as Candida parapsilosis as a cause of bloodstream infections. J Clin Microbiol 46: 374-376. 25. Lupetti, A., A. Tavanti, P. Davini, E. Ghelardi, V. Corsini, I. Merusi, A. Boldrini, M. Campa and S. Senesi (2002).Horizontal transmission of Candida parapsilosis candidemia in a neonatal intensive care unit. J Clin Microbiol 40: 2363-2369. 26. Mellmann, A., J. Cloud, T. Maier, U. Keckevoet, I. Ramminger, P. Iwen, J. Dunn, G. Hall, D. Wilson, P. Lasala, M. Kostrzewa and D. Harmsen (2008). Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria. J Clin Microbiol 46: 1946-1954. 27. Orsi, C. F., B. Colombari and E. Blasi (2010).Candida metapsilosis as the least virulent member of the 'C. parapsilosis' complex. Med Mycol 48: 1024-1033. 28. Pfaller, M. A., L. Boyken, R. J. Hollis, J. Kroeger, S. A. Messer, S. Tendolkar and D. J. Diekema (2008).In vitro susceptibility of invasive isolates of Candida spp. to anidulafungin, caspofungin, and micafungin: six years of global surveillance. J Clin Microbiol 46: 150-156. 29. Pfaller, M. A., D. J. Diekema, D. L. Gibbs, V. A. Newell, J. F. Meis, I. M. Gould, W. Fu, A. L. Colombo and E. Rodriguez-Noriega (2007).Results from the ARTEMIS DISK Global Antifungal Surveillance study, 1997 to 2005: an 8.5-year analysis of susceptibilities of Candida species and other yeast species to fluconazole and voriconazole determined by CLSI standardized disk diffusion testing. J Clin Microbiol 45: 1735-1745. 30. Posteraro, B., A. Ruggeri, E. De Carolis, R. Torelli, A. Vella, F. De Maio, W. Ricciardi, P. Posteraro and M. Sanguinetti (2013).Comparative evaluation of BD Phoenix and vitek 2 systems for species identification of common and uncommon pathogenic yeasts. J Clin Microbiol 51: 3841-3845. 31. Qian, J., J. E. Cutler, R. B. Cole and Y. Cai (2008).MALDI-TOF mass signatures for differentiation of yeast species, strain grouping and monitoring of morphogenesis markers. Anal Bioanal Chem 392: 439-449. 32. Ramage, G., J. P. Martinez and J. L. Lopez-Ribot (2006).Candida biofilms on implanted biomaterials: a clinically significant problem. FEMS Yeast Res 6: 979-986. 33. Richard A.Calderone and Cornelius J.Clancy(2012).The Epidemiology of invasive Candidiasis.Candida and Candidasis,2nd Edition.p 449-480. 34. Roetzer, A., T. Gabaldon and C. Schuller (2011). rom Saccharomyces cerevisiae to Candida glabratain a few easy steps: important adaptations for an opportunistic pathogen.FEMS Microbiol Lett 314: 1-9. 35. Ruan, S. Y. and P. R. Hsueh (2009). Invasive candidiasis: an overview from Taiwan. J Formos Med Assoc 108: 443-451. 36. Ruchel, R., F. de Bernardis, T. L. Ray, P. A. Sullivan and G. T. Cole (1992). Candida acid proteinases. J Med Vet Mycol 30 Suppl 1: 123-132. 37. Saiman, L., E. Ludington, J. D. Dawson, J. E. Patterson, S. Rangel-Frausto, R. T. Wiblin, H. M. Blumberg, M. Pfaller, M. Rinaldi, J. E. Edwards, R. P. Wenzel and W. Jarvis (2001). Risk factors for Candida species colonization of neonatal intensive care unit patients. Pediatr Infect Dis J 20: 1119-1124. 38. Stevenson, L. G., S. K. Drake, Y. R. Shea, A. M. Zelazny and P. R. Murray (2010).Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of clinically important yeast species. J Clin Microbiol 48: 3482-3486. 39. Tavanti, A., L. A. Hensgens, S. Mogavero, L. Majoros, S. Senesi and M. Campa (2010).Genotypic and phenotypic properties of Candida parapsilosis sensu strictu strains isolated from different geographic regions and body sites. BMC Microbiol 10: 203. 40. Tay, S. T., S. L. Na and J. Chong (2009).Molecular differentiation and antifungal susceptibilities of Candida parapsilosis isolated from patients with bloodstream infections.J Med Microbiol 58: 185-191. 41. Trofa, D., A. Gacser and J. D. Nosanchuk (2008).Candida parapsilosis, an emerging fungal pathogen. Clin Microbiol Rev 21: 606-625. 42. Trofa, D., L. Soghier, C. Long, J. D. Nosanchuk, A. Gacser and D. L. Goldman (2011). A rat model of neonatal candidiasis demonstrates the importance of lipases as virulence factors for Candida albicans and Candida parapsilosis.Mycopathologia 172: 169-178. 43. van Asbeck, E. C., Y. C. Huang, A. N. Markham, K. V. Clemons and D. A. Stevens (2007). Candida parapsilosis fungemia in neonates: genotyping results suggest healthcare workers hands as source, and review of published studies. Mycopathologia 164: 287-293. 44. Weems, J. J., Jr., M. E. Chamberland, J. Ward, M. Willy, A. A. Padhye and S. L. Solomon (1987). Candida parapsilosis fungemia associated with parenteral nutrition and contaminated blood pressure transducers. J Clin Microbiol 25: 1029-1032.
摘要: 
念珠菌菌血症病例在世界各醫院日益增加,尤其是免疫功能有缺陷或是重症的病人,需要接受免疫抑制劑或化學治療,長期的血液透析,以及全靜脈營養注射或是中央靜脈導管置放等侵入性醫療裝置,近平滑念珠菌群(Candida parapsilosis complex)藉由移生而造成病人的感染,甚至爆發群聚事件,因之念珠菌的鑑定與研究益形重要。Lehmann等人(1992)年利用randomly amplified polymorphic DNA 分析,將C. parapsilosis complex分為groupⅠ、II、Ⅲ。Tavanti等人(2005)年發現groupⅠ與group II及Ⅲ的Internal transcribed spacer(ITS1)基因序列相似度小於90%,認定他們為亞種,所以將group II 稱為Candida orthopsilosis,, groupⅢ則稱為Candida metapsilosis,此兩者的侵入性及毒性也有所不同。有鑑於鑑定方法推陳出新,本研究自2009年6月起至2014年8月止,收集來自中國醫藥大學附設醫院血液檢體分離出的C. parapsilosis complex,共101株。所有菌株皆以API ID32C商業套組鑑定結果為C. parapsilosis;樣品經蟻酸萃取後以基質輔助雷射脫附離子化-飛行時間(MALDI-TOF)質譜儀鑑定並分型,其結果為C. parapsilosis 75株(75/101)佔74%,C. orthopsilosis 11株(11/101)佔11 %,C. metapsilosis 15株(11/101)佔15 %。最後再以外顯子間填裝內含子之聚合酶連鎖反應(EPIC -PCR)驗證MALDI-TOF的結果,發現MALDI-TOF的結果與EPIC- PCR的結果100%符合。綜合前述本研究利用MALDI-TOF進行C. parapsilosis complex分型,進而證實MALDI-TOF能快速且準確區分C. parapsilosis complex。

Candida bacteremia cases increasing in various hospitals of the world, especially in immunocompromised, prior immunosuppressive therapy, chemotherapy, hemodialysis, total parenteral nutrition, central venous catheter injection , and other invasive medical devices, Candida parapsilosis complex caused by colonization and infection of patients, even the outbreak of nosocomial infection. In 1992, Lehmann et al. suggested that C. parapsilosis isolates can be divided into groupⅠ,Ⅱ, and Ⅲ on the basis of randomly amplified polymorphic DNA (RAPD) results. However, Tavanti et al. (2005) found C. parapsilosis groupⅠcompared with groupⅡand Ⅲ of DNA sequences similarities were less than 90% in the ITS1 sequence, and formally designate C. parapsilosis groups II and III as the new species C. orthopsilosis and C. metapsilosis on the grounds. This study included 101 C. parapsilosis complex strains identified among blood sample isolates collected from June 2009 to August 2014 in China Medical University Hospital. Identification of the C . parapsilosis was tested using API ID 32C .Formic acid extraction sample was identified by Matrix-assisted laser desorption / ionization time-of-flight (MALDI-TOF) mass spectrometry, the result for C. parapsilosis 75 strains (75/101) 74%, C. orthopsilosis 11 strains (11/101) 11%, C. metapsilosis 15 strains (11/101) 15%. Exon-primed intron-crossing polymerase chain reaction (EPIC-PCR) was used to verify the results of MALDI-TOF, Both of the MALDI-TOF findings and EPIC-PCR results with 100% compliance. This study proved that MALDI-TOF mass spectrometry for differentiation of C. parapsilosis complex, will become a rapid and accurate method to distinguish among C. parapsilosis complex in clinical laboratory.
URI: http://hdl.handle.net/11455/92623
其他識別: U0005-1508201511500600
Rights: 同意授權瀏覽/列印電子全文服務,2016-08-21起公開。
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