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Selection of Monoclonal Antibody Against Recombinant Grb2-SH2 Domain
|關鍵字:||乳癌;生長因子受體結合蛋白2;SH2 domains;Breast;Grb2;SH2 domains||引用:||1. Leistikow, B., Lung cancer rates as an index of tobacco smoke exposures: validation against black male approximate non-lung cancer death rates, 1969-2000. Prev Med, 2004. 38(5): 511-5. 2. Warnes, A.M., UK and western European late-age mortality: trends in cause-specific death rates, 1960-1990. Health Place, 1999. 5(1): 111-8. 3. Newbold, R.R., et al., Uterine carcinoma in mice treated neonatally with tamoxifen. Carcinogenesis, 1997. 18(12): 2293-8. 4. Boerner, J.L., et al., Grb2 regulation of the actin-based cytoskeleton is required for ligand-independent EGF receptor-mediated oncogenesis. Oncogene, 2003. 22(43): 6679-89. 5. Sommer, S. and S.A. Fuqua, Estrogen receptor and breast cancer. Semin Cancer Biol, 2001. 11(5): 339-52. 6. McPherson, K., C.M. Steel, and J.M. Dixon, ABC of breast diseases. Breast cancer-epidemiology, risk factors, and genetics. Bmj, 2000. 321(7261): 624-8. 7. van der Geer, P., T. Hunter, and R.A. 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Cancer was the leading cause of death in Taiwan. Breast cancer was the most prevalent cancer in female. Clinical breast cancer therapies include surgery, chemical and radial therapy. Several drugs were used for chemical therapy, however, these drug treatments have many side effects, even leading to some other cancers development. The drug with low side effect is needed to avoid these side effects. Antibody, from the immune system?has high specificity to a candidate specific cell or a molecul and becomes a potential drug for targeted therapy. Cancer development comes from abnormal growth mechanism from normal cells. According to previous study, Grb2 protein (Growth factor receptor-bound protein 2) with SH2 domain (Src-homology 2 domain) was connected to cancer cell proliferation. In this study, we tried to acquire a SH2 domin specific antibody to prevent cancer cell abnormal growth. MCF-7 cell was used and its genome was served as template to amplified Grb2 gene SH2 domain fragment to further insert this DNA fragment into expression plasmid. SH2 domain fragment of Grb2 was expressed in E.coli expression system. Expressed protein was gathered as antigen to immune the mouse. After elevated immune response, mice were screened and sacrifice to obtain antibody in sera. The B Cells were fused with NS1 myeloma cells to produce hybridoma. We got 2 limes of SH2 antibody producing hybridoma after the monoclonal selection. The 6H3 antibody was highly specific to SH2 protein and will be use to enginerring for scFv drug development in the future.
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