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標題: Inhibition of Eimeria tenella invasion on epithelial cells by edible Asteraceae herb – in comparison with coccidiostat ionophore salinomycin
作者: Wei-Chun Lee
關鍵字: 雞球蟲症;艾美球蟲屬;菊科植物;抑制入侵;coccidia;Eimeria;Asteraceae;invasion inhibition
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雞球蟲為一種隸屬於頂端複合蟲門中艾美球蟲屬,其生活史寄生於腸道上皮內,為現今家禽產業中分布最廣且造成嚴重經濟損失的疾病,但鑒於歐盟禁用化學藥物於飼料添加及疫苗亦無法提供足夠的保護性,因此發展新型且安全的抗球蟲添加劑並避免抗藥性益顯重要。我們實驗室先前證明利用一可食性菊科植物 (Asteraceae plant) 於飼料添加可有效降低機盲腸型球蟲感染後卵囊的排放量及影響球蟲生活史,且經多次繼代證明球蟲不會對本植物產生抗藥性,因此推測本菊科植物並非以直接殺滅蟲體來達到防治球蟲症效果。我們利用單一孢子囊繼代於雞隻而得單一基因型球蟲株,再經弱陰離子交換樹脂純化出無菌孢子體,於生物體外系統測試菊科萃取物對其存活率及抑制入侵上皮細胞的影響。結果顯示生物體外系統試驗,經菊科萃取物處理過的孢子體組別其上皮細胞入侵率 (8%) 低於未處理組 (27%) 並具統計學上顯著差異。另外利用propidium iodide (P.I.) 染色證明菊科萃取物並不會直接造成蟲體死亡且存活率 (99%) 跟salinomycin藥物對照組 (55%) 具統計學上顯著差異。本篇研究揭示了菊科植物於防治球蟲症的機制,可提供未來發展菊科植物於家禽現場應用和防治其它頂端複合蟲門的原蟲性疾病。

Coccidiosis is a disease caused by parasites of the genus Eimeria belonging to the phylum Apicomplexa with a complex life cycle, affecting mainly the intestinal tract of many species of mammals and birds. Anticoccidial drugs are widely used for the control of coccidiosis in the fowl which has inevitably led to the development of drug resistance. Using phytomedicine as a supplementation in feed to treat or control the coccidiosis in chickens is taken. The anticoccidial effect of an Asteraceae plant (AP) contained feed has been demonstrated in our laboratory, for example, lower fecal E. tenella oocysts excretion of chickens. To further determine the anticoccidial mechanism of the AP in chick, we use single-sporocyst infection to produce a pure strain of E. tenella and purify sterile sporozoites by the ion exchange chromatography. Results showed that in vitro the AP extract-treated sporozoites have significant lower invasion rates 8% in comparison with control group 27%. Meanwhile, we demonstrated AP extract has low toxicity to sporozoites in contrast to the ionophore salinomycin. Survival rate of AP extract-treated sporozoites is 99% and salinomycin-treated group is 55% by propidium iodide (P.I.) staining. The results indicated that AP of a new anticoccidial mechanism has a great potential on field application without causing drug resistance and residue problems.
其他識別: U0005-2408201512183000
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