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  2. 獸醫學院
  3. 獸醫學系所
Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/93053
標題: Expression of reduced folate carrier in normal canine tissues and lymphomas
還原葉酸載體在犬正常組織及淋巴瘤的表現
作者: Chun-Yuan Huang
黃俊元
關鍵字: 無;NO
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摘要: 
還原葉酸載體(reduced folate carrier, RFC)是細胞輸入葉酸及抗葉酸藥物的最主要運輸物，其在人和齧齒動物各種組織的基因表現已被建立，至今在犬卻少有研究 本研究的主要目的包含 (一) 建構能增幅犬部分 RFC 基因的聚合酶鏈鎖反。應(polymerase chain reaction, PCR)引子及 (二) 偵測犬正常和淋巴瘤組織的 RFC基因表現量。研究樣本包含 5 隻安死術犬而得的 35 種正常組織及 22 個犬淋巴瘤組織。由這些組織萃取 RNA 後反轉錄為 cDNA，再利用 PCR 增幅出 RFC 基因。將電泳下 RFC 條帶亮度與甘油醛-3-磷酸脱氢酶(glyceraldehyde 3-phosphatedehydrogenase, GAPDH)相除，獲得定量的 RFC 基因表現量。 研究結果顯示 RFC在犬正常組織間幾乎都有表現，但表現程度不一，於中樞神經系統及免疫系統可見比平均更高的表現量 犬淋巴瘤組織的 RFC 表現量則遠低於正常淋巴組織(P <。0.001)，且多中心型 B 細胞淋巴瘤、多中心型 T 細胞淋巴瘤及皮膚型 T 細胞淋巴瘤之表現量並無顯著差異。整體而言，RFC 於各種組織間的廣泛分佈支持其在體內葉酸平衡的重要性 而犬淋巴瘤組織的低表現量可能因複雜的機制應組織及，環境的不同做調整，尚須進一步證明。

Reduced folate carrier (RFC) is the main carrier which transports folates and antifolates into the cell. RFC gene expression in human and rodent tissues had been established, however, it has not been revealed in dogs yet. The aims of the present study were: (1) to construct specific PCR primers for amplification of canine RFC gene; (2) to assess RFC gene expressions in normal canine and lymphomas tissues. Thirty-five kinds of normal canine tissues were collected from five euthanized dogs and twenty-two lymphomas were enrolled. Total RNA were extracted from normal and lymphoma tissues, and were transcripted reversely to complementary DNA, and then were evaluated for RFC expressions using polymerase chain reaction. RFC expression levels were obtained from quantified bands of RFC divided by glyceraldehyde 3-phosphate dehydrogenase. It was shown that RFC transcripts were expressed ubiquitously but various levels. Higher expressions were observed in central nervous and immune system than the median of all tested tissues. Expressions of RFC in normal lymphoid tissues were significantly higher when compared to B- or T-cell lymphomas (P < 0.001). And there were no significant difference among multicentric B-cell lymphomas, multicentric T-cell lymphomas, and cutaneous T-cell lymphomas. We concluded that the ubiquitous distribution with various levels of RFC in 35 kinds of normal canine tissues and malignant tissues. Higher expressions were observed in central nervous, immune, and urinary systems than others. Stomach had the highest RFC expression, followed by cerebellum and brain stem, with very low levels in duodenum and cardiac muscle. And expressions of RFC were significantly lower in all cases of lymphomas when compared to normal lymphatic organs (thymus, palatine tonsil, lymph node), which composed mainly of lymphocytes.
URI: http://hdl.handle.net/11455/93053
其他識別: U0005-2105201514540500
Rights: 不同意授權瀏覽/列印電子全文服務
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