Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/93075
標題: 家禽里奧病毒足底接種後之足底病理變化及炎症細胞的消長
Pathological Changes of Footpad and Fluctuation of Inflammatory Cells after Arthrotropic Avian Reovirus Footpad Inoculation
作者: Tsung-Ching Liu
劉宗璟
關鍵字: 家禽里奧病毒;S1133株;炎症反應;異嗜球;Avian reovirus;S1133 strain;inflammation;heterophil
引用: 全國法規資料庫。雞里奧病毒活毒疫苗檢驗標準。第七十四節。2013。(available online: http://law.moj.gov.tw/LawClass/LawAll.aspx?PCode=M0130018)。 吳典諺。實驗結果。脾臟摘除之雛雞對家禽里奧病毒2408接種後之肝臟病變評估。國立中興大學碩士論文,17-21,2013 沈品均。結果。家禽里奧病毒感染致病機制之探討。國立中興大學博士論文,27-29,2013。 李崇道。炎症。獸醫病理學。初版。台北,黎明文化事業股份有限公司,171-226,2006。 呂榮修。病毒性關節炎/里奧病毒性腱鞘炎。禽病診斷彩色圖譜。初版。台北,中華民國養雞協會,75-81,1995。 施文凱。結果。應用即時定量聚合酶鏈反應偵測雞隻感染家禽里奧病毒2408之細胞素變化。國立中興大學碩士論文,23-26,2008 Ackermann MR. Inflammation and healing. In: Zachary JF, McGavin MD, eds. Pathologic basis of veterinary disease. 5th ed. Elservier, St. Loius, 89-146, 2012. al Afaleq A, Jones RC. A comparison of single and repeated oral infection of chicks with two avian reoviruses. Res Vet Sci 57: 96-99, 1994. Al-Muffarej SI, Savage CE, Jones RC. Egg transmission of avian reoviruses in chickens: comparison of a trypsin-sensitive and a trypsin-resistant strain. Avian Pathol 25: 469-480, 1996. Andreasen CB. Staphylococcosis. In: Saif YM, Fadly AM, Glisson JR, McDougald LR, Nolan LK, Swayne DE, eds. Diseases of poultry. 12th ed. Blackwell, Iowa, 892-896, 2008. Andreasen CB, Latimer KS. Cytochemical staining characteristics of chicken heterophils and eosinophils. Vet Clin Pathol 19: 51-54, 1990. Andreasen JR, Andreasen CB, Anwer M, Sonn AE. Chicken heterophil chemotoxis using Staphylococcus-generated chemoattractants. Avian Dis 37: 835-838, 1993 Andreasen JR, Andreasen CB, Anwer M, Sonn AE. Heterophil chemotaxis in chickens with natural staphylococcal infections. Avian Dis 37: 284-289, 1993. Barker KA, Hampe A, Stoeckle MY, Hanafusa H. Transformation-associated cytokine 9E3/CEF4 is chemotactic for chicken peripheral blood mononuclear cells. J Virol 67: 3528-3533, 1993. Barnes HJ, Fletcher OJ, Abdul-Aziz T. Hemic system. In: Fletcher OJ, Abdul-Aziz T, eds. Avian histopathology. 3rd ed. American Association of Avian Pathologist, Florida, 1-16, 2008. Barta V, Springer WT, Millar DL. A comparison of avian and mammalian cell cultures for the propagation of avian reovirus WVU 2937. Avian Dis 28: 216-223, 1984. Benavente J, Martinez-Costas J. Avain reovrirus: structural and biology. Virus Res 123: 105-119, 2007. Bodelon G, Labrada L, Martinez-Costas J, Benavente J. The avian reovirus genome segment S1 is a functionally tricistronic gene that expresses one structural and two nonstructural proteins in infected cells. Virology 290: 181-191, 2001. Calnek BW. Avian encephalomyelitis. In: Saif YM, Fadly AM, Glisson JR, McDougald LR, Nolan LK, Swayne DE, eds. Diseases of poultry. 12th ed. Blackwell, Iowa, 430-438, 2008. Carlson CS, Weisbrode SE. Bones, joints, tendons, and ligaments. In: Zachary JF, McGavin MD, eds. Pathologic basis of veterinary disease. 5th ed. Elservier, St. Loius, 964-971, 2012. Cavanagh D, Gelb JJ. Infectious bronchitis. In: Saif YM, Fadly AM, Glisson JR, McDougald LR, Nolan LK, Swayne DE, eds. Diseases of poultry. 12th ed. Blackwell, Iowa, 117-130, 2008. Chen BY, Hosi S, Nunoya T, Itakura C. Histopathology and immunohistochemistry of renal lesions due to infectious bronchitis virus in chicks. Avian Pathol 25: 269-283, 1996. Chen BY, Itakura C. Cytopathology of chick renal epithelial cells experimentally infected with avian infectious bronchitis virus. Avian Pathol 25: 675-690, 1996. Chen PN, Liu HJ, Shein JH, Lee LH. Antibody responses against avian reovirus nonstructural protein sigmaNS in experimentally virus-infected chickens monitored by monoclonal antibody capture enzyme-linked immunosorbent assay. Res Vet Sci 76: 219-225, 2004. Clark FD, Ni Y, Collisson EW, Kemp MC. Characterization of avian reovirus strain-specific polymorphisms. Avian Dis 34: 304-314, 1990. Coltey N, Bucy RP, Chen CH, Cihak J, Lȍsch U, Char D, Le Douarin NM, Copper MD. Analysis of the first two waves of thymus homing stem cells and their T cell progeny in chick-quail chimeras. J Exp Med 170: 543-557, 1989. Dutta SK, Pomeroy BS. Isolation and characterization of an enterovirus from baby chicks having an enteric infection II. Physical and chemical characteristics and ultrastructure. Avian Dis 11: 9-14, 1967. Fahey JE, Crawley JF. Studies on chronic respiratory diseases of chickens II. Isolation of a virus. Can J Comp Med 18: 13-21, 1954. Fan YH, Lin CD, Chiou SH, Chow KC, Chi YS, Lee LH, Shien JH, Shieh HK. Differential expression of U2AF35 in the arthritic joint of avian reovirus-infected chicks. Vet Immunol Immunopathol 114: 49-60, 2006. Fletcher OJ, Abdul-Aziz T, Barnes HJ. Skeletal system. In: Fletcher OJ, Abdul-Aziz T, eds. Avian histopathology. 3rd ed. American Association of Avian Pathologist, Florida, 59-63, 2008. Giambrone JJ, Solano W. Serologic comparison of avian reovirus isolates using virus neutralization and an enzyme-linked immunosorbent assay. Avian Dis 32: 678-680, 1988. Glass SE, Naqi SA, Hall CF, Kerr KM. Isolation and characterization of a virus associated with arthritis of chickens. Avian Dis 17: 415-424, 1973. Glisson JR, Hofacre CL, Christensen JP. Fowl Cholera. In: Saif YM, Fadly AM, Glisson JR, McDougald LR, Nolan LK, Swayne DE, eds. Diseases of poultry. 12th ed. Blackwell, Iowa, 739-754, 2008. Golemboski KA, Whelan J, Shaw S, Kinsella JE, Dietert RR. Avian inflammatory macrophage function: shifts in arachidonic acid metabolism, respiratory burst, and cell-surface phenotype during the response to Sephadex. J Leukoc Biol 48: 495-501, 1990. Goodwin MA, Davis JF, McNulty MS, Brown J, Player EC. Enteritis (so-called runting stunting syndrome) in Georgia broiler chicks. Avian Dis 37: 451-458, 1993. Gough RE, Alexander DJ, Collins MS, Lister SA, Cox WJ. Routine virus isolation or detection in the diagnosis of diseases in birds. Avian Pathol 17: 893-907, 1988. Gouvea V, Schnitzer TJ. Pathogenicity of avian reoviruses: examination of six isolates and a vaccine strain. Infect Immun 38: 731-738, 1982. Gouvea V, Schnitzer TJ. Polymorphism of the genomic RNAs among the avian reoviruses. J Gen Virol 61: 87-91, 1982. Guneratne JR, Jones RC, Georgiou K. Some observations on the isolation and cultivation of avian reoviruses. Avian Pathol 11: 453-462, 1982. Guy JS, Garcia M. Laryngotracheitis. In: Saif YM, Fadly AM, Glisson JR, McDougald LR, Nolan LK, Swayne DE, eds. Diseases of poultry. 12th ed. Blackwell, Iowa, 137-146, 2008. Harmon BG. Avian heterophils in inflammation and disease resistance. Poul Sci 77: 972-977, 1998. Hou HS, Su YP, Shien HK, Lee LH. Monoclonal antibodies against different epitopes of nonstructural protein sigmaNS of avian reovirus S1133. Virology 30: 168-175, 2001. Hieronymus DR, Villegas P, Kleven SH. Identification and serological differentiation of several reovirus strains isolated from chickens with suspected malabsorption syndrome. Avian Dis 27: 246-251, 1983. Hlinak A, Muller T, Kramer M, Muhle RU, Liebherr H, Ziedler K. Serological survey of viral pathogens in bean and white-fronted geese from Germany. J Wildl Dis 34: 479-486, 1998. Huang HB, Liu YX, Hou Y, Wen L, Ge XH, Peng KM, Liu HZ. Distribution patterns of stromal eosinophil cells in chick thymus during postnatal development. Vet Immunol Immunopathol 153: 123-127, 2013. Ignjatovic J, Ashton DF, Reece R, Scott P, Hooper P. Pathogenicity of Australian strains of avian infectious bronchitis virus. J Comp Pathol 126: 115-123, 2002. Islam MR, Jones RC, Kelly DF. Pathogenesis of experimental reovirus tenosynovitis in chickens: influence of the route of infection. J Comp Pathol 98: 325-336, 1988. Johnson DC. Diagnosis, pathology, and etiology of tenosynovitis in broilers and broiler breeders. Avian Dis 16: 1067-1072, 1972. Jones RC. Reovirus infections. In: Saif YM, Fadly AM, Glisson JR, McDougald LR, Nolan LK, Swayne DE, eds. Diseases of poultry. 12th ed. Blackwell, Iowa, 309-328, 2008. Jones RC, Georgiou K. Reovirus-induced tenosynovitis in chickens the influence of age at infection. Avian Pathol 13: 441-457, 1984. Jones RC, Grorgiou K. The temporal distribution of an arthrotropic reovirus in the leg of the chicken after oral infection. Avian Pathol 14: 75-85, 1985. Jones RC, Islam MR, Kelly DF. Early pathogenesis of experiment reovirus infection in chickens. Avian Pathol 18: 239-253, 1989. Jones RC, Jordan TW, Lioupis S. Characteristics of reovirus isolated from rupture gastrocnemius tendons of chickens. Vet Res 96: 153-154, 1975. Jones RC, Kibenge FS. Reovirus-induced tenosynovitis in chickens: the effect of breed. Avian Pathol 13: 511-528, 1984. Kass L, Harrison GJ, Lindheimer C. A new stain for identification of avian leukocytes. Biotech Histochem 77: 201-206, 2002. Kibenge FS, Wilcox GE. Tenosynovitis in chickens. Vet Bull 53: 431-444, 1983. Ke GM, Cheng HL, Ke LY, Ji WT, Chulu JL, Liao MH, Liu HJ. Development of a quantitative Light Cycler real-time RT-PCR for detection of avian reovirus. J Virol Methods 133: 6-13, 2006. Kerr KM, Olson NO. Control of infectious synovitis. 14. The effect of age of chickens on the susceptibility to three agent. Avian Dis 8: 256-263, 1964. Kerr KM, Olson NO. Pathology of chickens experimentally inoculated or contact-infected with an arthritis-producing virus. Avian Dis 13: 729-745, 1969. Lee LH. Wang YH, Shien JH. Serological characterization of reoviruses isolated from avian species in Taiwan. J Chin Soc Vet Sci 18: 69-77, 1992. Levisohn S, Gur-Lavie A, Weisman J. Infectious synovitis in turkeys: Isolation of tenosynovitis virus-like agent. Avian Pathol 9: 1-4, 1980. Lin HY, Chuang ST, Chen YT, Shih WL, Chang CD, Liu HJ. Avian reovirus-induced apoptosis related to tissue injury. Avian Pathol 36: 155-159, 2007. Lin YL, Shen JH, Lee LH. A monoclonal antibody-based competitive enzyme-linked immunosorbent assay for detecting antibody production against reovirus protein sigmaA. J Virol Methods 136: 71-77, 2006. Liu HJ, Kuo LC, Liao MH, Lein YY. Development of an ELISA for detections of antibodies to avian reovirus in chickens. J Virol Methods 102: 129-138, 2002. Liu HJ, Lee LH, Hsu HW, Kuo LC, Liao MH. Molecular evolution of avian reovirus: evidence for genetic diversity and reassortment of the the S-class genome segments and multiple cocirculating lineages. Virology 314: 336-349, 2003. Liu HJ, Liao MH, Chang CD, Chen JH, Lin MY, Tung MC. Comparison of two molecular techniques for detection of avian reoviruses in formalin-fixed, paraffin embedded chicken tissues. J Virol Methods 80: 197-201, 1999. Malkinson M, Perk K, Weisman Y. Reovirus infection of young Muscovy ducks (Cairina moschata). Avian Pathol 10: 433-440, 1981. Mandelli G, Rampin T, Finazzi M. Experimental reovirus hepatitis in newborn chicks. Vet Pathol 15: 531-543, 1978. McFerran JB, Cannor TJ, McCracken RM. Isolation of adenoviruses and reoviruses from avian species other than domestic fowl. Avian Dis 20: 519-524, 1976. McFerran JB, McCracken RM, Connor TJ, Evans RT. Isolation of viruses from clinical outbreaks of inclusion body hepatitis. Avian Pathol 5: 315-324, 1976. Menendez NA, Calnek BW, Cowen BS. Experimental egg-transmission of avian reovirus. Avian diseases 19: 104-111, 1975. Montali RJ. Comparative pathology of inflammation in the higher vertebrates (reptiles, birds and mammals). J Comp Pathol 99: 1-26, 1988. Morin MJ, Warner A, Fields BN. Reovirus infection in rat lung as a model to study the pathogenesis of viral pneumonia. J Virol 70: 541-548, 1996. Morrison TE, Oko L, Montgomery SA, Whitmore AC, Lotstein AR, Gunn BM, Elmore SA, Heise MT. A mouse model of chikungunya virus-induced musculoskeletal inflammatory disease: evidence of arthritis, tenosynovitis, myositis, and persistence. Am J Pathol 178: 32-40, 2011. Myers RK, Zachary JF, McGavin MD. Cellular adaptions, injury, and death: morphologic biochemical, and genetic bases. In: Zachary JF, McGavin MD, eds. Pathologic basis of veterinary disease. 5th ed. Elservier, St. Loius, 13-26, 2012. Nersessian BN, Goodwin MA, Page RK, Kleven SH. Studies on orthoreoviruses isolated from young turkeys. II. Virus distribution in organs and serological response of poults inoculated orally. Avian Dis 29: 963-969, 1985. Ni Y, Kemp MC. A comparative study of avian reovirus pathogenicity: virus spread and replication and induction of lesions. Avian Dis 39: 554-566, 1995. Oder EM, Page RK, Fletcher OJ, Villegas P. Pale bird syndrome-clinical picture and etiology. J Am Vet Med Assoc 179: 273, 1981. Olson NO. Transmissible synovitis of poultry. Lab Invest 8: 1384-1393, 1959. Olson NO, Kerr KM. Some characteristics of an avian arthritis viral agent. Avian Dis 10: 470-476, 1966. Olson NO, Sahu SP. Avian viral arthritis: antigentic types and immune response. Am J Vet Res 36: 245-247, 1975. Olson NO, Shelton DC, Munro DA. Infectious synovitis control by medication; effect of strain differences and pleuropneumonia-like organisms. Am J Vet Res 18: 735-739, 1957. Page RK, Fletcher OJ, Rowland GN, Gaudry D, Villegas P. Malabsorption syndrome in broiler chickens. Avian Dis 26: 618-624, 1982. Pertile TL, Walser MM, Sharma JM, Shivers JL. Immunohistochemical detection of lymphocyte subpopulations in the tarsal joints of chickens with experimental viral arthritis. Vet Pathol 33: 303-310, 1996. Purcell DA. The ultrastructural changes produced by infectious laryngotracheitis virus in tracheal epithelium of the fowl. Res Vet Sci 12: 455-458, 1971. Rath, NC, Huff GR, Balog JM, Huff WE. Fluorescein isothiocynate staining and characterization of avian heterophils. Vet Immunol Immunopathol 64: 83-95, 1998. Reed LJ, Muench H. A simple method of estimating fifty percent endpoints. Am J Hyg 27: 493-497, 1938. Rhoades KR, Rimler RB. Toxicity and virulence of capsular serogroup D Pasteurella multocida strain isolated from turkey. J Am Med Assoc 192: 1790, 1988. Riddell C. Respiratory system. In: Avian histopathology. 2nd ed. American Association of Avian Pathologists, Pennsylvania, 37-45, 1987. Roessler DE, Rosenberger JK. In vitro and in vivo characterization of avian reoviruses. III. Host factors affecting virulence and persistence. Avian Dis 33: 555-565, 1989. Rosenberger JK, Sterner FJ, Botts S, Lee KP, Margolin A. In vitro and in vivo characterization of avian reoviruses. I. Pathogenicity and antigenic relatedness of several avian reovirus isolates. Avian Dis 33: 535-544, 1989. Schneider K, Klaas R, Kaspers B, Staeheli P. Chicken interleukin-6. cDNA structure and biological properties. Eur J Biochem 268: 4200-4206, 2001. Schnitzer TJ, Ramos T, Gouvea V. Avian reovirus polypeptides: analysis of intreacellular virus-specified products, virions, top component, and cores. J Virol 43: 1006-1014, 1982. Sekellick MJ, Ferrandino AF, Hopkins DA, Marcus PI. Chicken interferon gene: cloning, expression, and analysis. J Interferon Res 14: 71-79, 1994. Sevoian M, Levine PP. Effects of infectious bronchitis on the respiratory tracts, egg production, and egg quality of laying chickens. Avian Dis 1: 136-164, 1957. Shien JH, Yin HS, Lee LH. An enzyme-linked immunosorbent assay for the detection of antibody to avian reovirus by using protein sigma B as the coating antigen. Res Vet Sci 69: 107-112, 2000. Shivaprasad HL, Barrow PA. Pullorum disease and fowl typhoid. In: Saif YM, Fadly AM, Glisson JR, McDougald LR, Nolan LK, Swayne DE, eds. Diseases of poultry. 12th ed. Blackwell, Iowa, 620-631, 2008. Suganuma Y. Histopathology studies of serositis of pullorum disease. Jpn J Vet Sci 22: 175-182, 1960. Swaggerty CL, Kogut MH, Ferro PJ, Rothwell L, Pevzner IY, Kaiser P. Differential cytokine mRNA expression in heterophils isolated from Salmonella-resistant and -susceptible chickens. Immunology 113: 139-148, 2004. Tang KN, Fletcher OJ. Application of the avidin-biotin-peroxidase complex (ABC) technique for detection avian reovirus in chickens. Avian Dis 31: 591-596, 1987. Tang KN, Fletcher OJ, Villegas P. Comparative study of the pathogenicity of avian reoviruser. Avian Dis 31, 577-583, 1987. Takase K, Nonaka F, Yamamoto M, Yamada S. Serologic and pathogenetic studies on avian reoviruses isolated in Japan. Avian Dis 31: 464-469, 1987. Thompson K. Bones and joints. In: Maxie MG, ed. Jubb, Kennedy and Palmer's pathology of domestic animals. 5th ed. Elsevier Saunders, Philadelphia, 158-184, 2007. Tizard IR. Innate immunity: proinflammatory and antimicrobial mediators. Innate immunity: neutrophils and phagocytosis. In: Veterinary immunology. 9th ed. Elsevier, St. Louis, 21-40, 2013. Urbano P, Urbano FG. The Reoviridae family. Comp Immunol Microbiol Infect Dis 17: 151-161, 1994. van der Heide L. The history of avian reovirus. Avian Dis 44: 638-641, 2000. van der Heide L, Geissler J, Bryant ES. Infectious tenosynovitis: serologic and histopathologic response after experimental infection with a Connecticut isolate. Avian Dis 18: 289-296, 1974. van der Heide L, Kalbac M. Infectious tenosynovitis (viral arthritis): characterization of a Connecticut viral isolation as a reovirus and evidence of viral egg transmission by reovirus-infected broiler breeders. Avian Dis 19: 683-688, 1975. van der Heide L, Kalbac M, Hall WC. Infectious tenosynovitis (viral arthritis): influence of maternal antibodies on the development of tenosynovitis lesions after experimental infection by day-old chickens with tenosynovitis virus. Avian Dis 20: 641-648, 1976. van Loon AA, Koopman HC, Kosman W, Mumczur J, Szeleszczuk O, Karpinska E, Kosowska G, Lutticken D. Isolation of a new serotype of avian reovirus associated with malabsorption syndrome in chickens. Vet Q 23: 129-133, 2001. Varela R, Benavente J. Protein coding assignment of avian reovirus strain S1133. J Virol 68: 6775-6777, 1994. Walker ER, Friedman MH, Olson NO. Electron microscopic study of an avian reovirus that causes arthritis. J Ultrastruct Res 41: 67-79, 1972. Weining KC, Sick C, Kaspers B, Staeheli P. A chicken homolog of mammalian interleukin-1 beta: cDNA cloning and purification of active recombinant protein. Eur J Biochem 258: 994-1000, 1998. Wigley P, Hulme SD, Bumstead N, Barrow PA. In vivo and in vitro studies of genetic resistance to systemic salmonellosis in the chicken encoded by the SAL1 locus. Microbes Infect 4: 1111-1120, 2002. Wood GW, Nicholas RA, Hebert CN, Thornton DH. Serological comparisons of avian reoviruses. J Comp Pathol 90: 29-38, 1980. Wood GW, Thornton DH. Experimental infetection of broiler chickens with an avian reovirus. J Comp Pathol 91: 69-76, 1981. Wong RA, Tellez GI, Valladares J, Hargis BM. Pathogenicity of Salmonella Gallinarum on an experimental infection of one-day-old broiler chicks. Poultry Sci 75: 44, 1996. Xie Z, Fadl AA, Gishick T, Khan MI. Amplification of avian reovirus RNA using the reverse transcriptase-polymerase chain reaction. Avian Dis 41: 654-660, 1997. Yin HS, Lee LJ. Charaterization of avian reovirus non structural protein sigmaNS synthesized in Escherichia coli. Virus Res 67: 1-9, 2000. Young B, Lowe JS, Stevens A, Heath JW. Skeletal tissues. In: Wheater's functional histology: a text and colour atlas. Churchill Livingstone, Philadelphia, 202-206, 2006.
摘要: 
以往家禽里奧病毒所造成的病毒性關節炎在野外感染後所觀察的病變皆以跗關節為主。而學者們在實驗模式接種下(含足底)也依野外感染的方式觀察跗關節,卻少有人觀察足底接種後的足底病變。本實驗先將家禽里奧病毒S1133株進行病毒培養複製再計算力價後以2000 TCID50的劑量共10 μl接種左肢足底,並觀察足底的病理變化以及炎症細胞的消長情形,最後再以免疫組織化學染色去標定家禽里奧病毒的複製訊號以及FITC染色去標定異嗜球;以接種10 μl滅菌PBS當作控制組。結果可觀察到足底接種病毒後有潮紅腫脹的現象,但潮紅會在接種後第5至6天逐漸消退而只剩下腫脹。鏡檢下,充鬱血情形與肉眼病變結果相呼應。此外除了看到淺層滑膜及纖維滑膜細胞的活化後增生肥大外,也可在接種處及趾骨與趾關節交接處有炎症細胞浸潤,一開始以淋巴球浸潤為主,但接種後第2天則逐漸改以異嗜球浸潤,直到接種後第5天,轉呈現少量至中等量的淋巴球浸潤,此外另可在接種後第3天開始有淋巴濾泡於血管周圍形成,而接種處引起的炎症反應會比骨腱交接處嚴重,其原因可能與病毒量及血管分布的多寡有關。在使用FITC染色及免疫組織化學染色計算出異嗜球的絕對數量並進行病變指數評分,可見接種後1.5至3.5天異嗜球的數量達到最高而在接種後第6天開始只可見少數異嗜球;此外,異嗜球並非家禽里奧病毒複製場所,而足底的滑膜細胞才是其標的細胞。關節囊液會在接種後第1至6天觀察到大量異嗜球伴隨少量脫落的滑膜細胞,之後異嗜球大量消退,該現象與野外感染的跗關節內關節囊液所觀察到的結果相類似。為了釐清炎症反應及異嗜球的趨化非受到病毒培養之細胞質內容物及病毒顆粒本身所造成,另外進行左肢足底接種培養過細胞的培養液及使用紫外光不活化後的病毒液各10 μl,結果顯現除了在接種後第1至2天有觀察到輕微的局部炎症反應外,並無觀察到類似接種家禽里奧病毒等之滑膜細胞活化或異嗜球趨化等病變。故認為家禽里奧病毒接種後所引起的炎症反應是細胞受到病毒感染後所引起,且炎症細胞及滑膜細胞並不會特別受到細胞質內容物或是病毒顆粒本身而引致趨化及活化。然而要了解異嗜球在整個致病機轉上所扮演的角色,則有待後續研究進行。

In the past, the lesion observed in chicken infected by wild strand virus of avian reovirus was confirned to the tarsal joint. In experiment models, scholars observed tarsal joint leion rather than foot pad lesion even after footpad inoculation. The aim of this experiment is to understand the pathological changes of footpad and the fluctuation of inflammatory cells after arthrotropic ARV S1133 footpad inoculation. The footpad appeared red and swollen after day post-inoculation (DPI) 5-6 then only swelling was noticed. Hyperemia and congestion were evident microscopically wihich concur with redness of the gross lesion. In addition to synovial cells activation evident by hyperplasia and hypertrophy, there were inflammatory cells at the inoculation site and the area between digital bone and digital flexor tendon. At first, majority population of the cells were lymphocytes, then shifted to heterophils infiltration after DPI 2-5 and at last replaced with mild to moderate lymphocytic infiltration starting after DPI 6. Moreover, perivascular lymphoid nodules were formed after DPI 3. The lesion at the inoculation site was more severe than the area between digital bone and digital flexor tendon which might be related to virus quantity and vascular richness. The result of the FITC stain and immunohistochemical stain showed highest count of heterophils after DPI 1.5-3.5 and only few heterophils were seen after DPI 6. Furthermore, synovial cells were the target cells of ARV replication rather than heterophils. The synovial fluid consisted of heterophils and few exfoliated synovial cells after DPI 1-6 then followed by drastic decrease in count. Another experiment using cell medium (used for viral incubation) and inactivated viral fluid for inoculation was pursued in order to clarify whether the inflammation and heterophil chemotaxis were induced by cytoplasmic content/viral particles or viral infection. The result showed that there were no similar pathological changes except mild inflammation after DPI 1-2. Therefore, inflammation after inoculation was induced by ARV infection rather than cytoplasmic content or virus particles. However, the exact role of heterophils responsible for the pathogenesis of ARV infection needs further investigation.
URI: http://hdl.handle.net/11455/93075
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