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標題: Functional Analysis of CdtABC Toxin from Avibacterium paragallinarum and Its Application 
to Vaccine Development
雞傳染性鼻炎菌 CdtABC 毒素之功能分析及其在疫苗研發之應用
作者: 林佳君
Chia-Chun Lin
關鍵字: 雞傳染性鼻炎菌;細胞致死腫脹毒素;Avibacterium paragallinarum;CdtABC
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Infectious coryza (IC), caused by Avibacterium paragallinarum, is an important respiratory disease of chicken that may lead to growth retardation and reduced egg production. The disease causes substantial economic losses in many countries of the world. Av. paragallinarum is a Gram-negative, facultatively anaerobic, rod shaped bacteria in the family of Pasteurellaceae. Cytolethal distending toxin (CDT) is a highly conserved exotoxin produced by a number of Gram-negative bacteria. CDT kills the host cells by inhibiting cell division and causing apoptosis. In this study, CDT-deficient mutants derived from strains TW94 and TW07 of Av. paragallinarum were constructed by using the TargeTron gene knockout system. The CDT-deficient mutants were characterized to investigate the role that CDT may play in the virulence of Av. paragallinarum. Further, recombinant CDT proteins were produced and used a vaccine antigen against Av. paragallinarum. The result showed that recombinant CDT proteins have potential to be used as vaccine antigens against Av. paragallinarum.

雞傳染性鼻炎菌 (Avibacterium paragallinarum, Av. paragallinarum) 為引起雞傳染性可利查 (Infectious coryza, IC) 之病原菌,此病又可稱作 雞傳染性鼻炎,為雞隻重要的上呼吸道感染疾病,可造成肉雞體重下降 淘汰率增加及蛋雞產蛋率下降,造成全球經濟嚴重的損失。雞傳染性鼻 炎菌為格蘭氏陰性菌細菌、兼性厭氧、桿狀型態的巴氏德桿菌家族,細 胞致死腫脹毒素 (CDT) 是許多革蘭氏陰性細菌的重要毒素蛋白,所產 生的高度保留外毒素因子,會影響宿主細胞分裂停滯進而走向凋亡。在 本研究中,在雞傳染性鼻炎菌的 TW07 及 TW94 菌株中使用商業套組 TargeTron 使 CdtB 基因不活化成為 CDT 基因缺損株,比較野生株與 突變株之差異,探討在雞傳染性鼻炎菌的毒力因子中扮演重要的角色, 另外利用表現重組蛋白技術表現出 CDT 重組蛋白,進而針對雞傳染性 鼻炎菌發展次單位疫苗。本研究結果發現 CDT 基因缺損株之毒素被破 壞後,對於細胞腫脹的程度明顯的減少,另一方面動物實驗結果發現, CDT 重組蛋白,可作為雞傳染性鼻炎菌之疫苗抗原。
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