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Column Elution Chromatography Coupled with Supercritical Carbon Dioxide Antisolvent Precipitation of Carotenoids-rich Particulates from Dunaliella salina
|關鍵字:||杜莎藻;反式-b-胡蘿蔔素;9-順-b -胡蘿蔔素;管柱純化;超臨界二氧化碳抗溶沉澱;應答曲面實驗設計;微米級顆粒;Dunaliella salina;trans-b-carotene;9-cis-b -carotene;Column elution chromatography;Supercritical anti solvent precipitation;Response surface method design;micronized precipitates||引用:||1.呂鋒洲, “杜莎藻的保健功效 富含天然綜合類葫蘿蔔素,” 立德文化有限公司 (2008) 2.行政院衛生署, 食品添加物使用範圍及限量暨規格標準- 8添加劑, 中華民國97年11月20日衛署食字第0970405591號令發布, 中華民國98年1月22日衛署食字第0980400197號令修正。 3.洪祥恩,“擬球藻養殖、類胡蘿蔔素萃取、及高含量玉米黃素奈米粉粒體製備,” 碩士論文, 中興大學化學工程學系 (2011) 4.Ahmad A.L., C.Y. Chan, S.R. Abd Shukor, M.D. Mashitah, “Optimization of carotene recovery from extracted oil of POME by adsorption chromatography using response surface methodology,” Separation and Purification Technology, 73, 279-285 (2010) 5.Ana R.Q., H.S. Costa, “Analysis of carotenoids in vegetable and plasma samples: A review,” Journal of Food Composition and Analysis, 19, 97–111 (2006) 6.Aviv S., A. Harari, L. Hananshvili, H. Cohena, R. Bitzur, T. Luvish, E. Ulmana, M. Golan, A. B. Amotz, D. Gavish, Z. Rotstein, D. 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This work investigated column elution chromatography of ultrasonic solvent extracts coupled with supercritical anti-solvent precipitation of carotenoids-containing particulates from microalgal Dunaliella Salina species. The extract contained carotenoids ranged from 61.3mg to 72.5mg using ultrasonic stirred ethyl ether or tetrahydrofuran (THF) extraction. When 6L ethyl alcohol was used in a reverse-phase column to elute 2g of the ethyl ether extract, the purity of trans-b-carotene attained 624.9mg/g, and the recovery was 84.6%. When 10L of ethyl alcohol employed for eluting the THF extract, the purity of trans-b-carotene increased to 823.6 mg/g with a recovery of 86.2%. Supercritical anti-solvent of ethyl ether solution did not enhance the purity of carotenoids, implied that carbon dioxide dissolves more carotenoids than chlorophylls into high-pressure solution. However, supercritical anti-solvent of THF solution at 160bar and 318K produced powdered particulates with purity of carotenoids above 90%. Subsequently, a center composited schemed response surface method designed supercritical anti-solvent precipitation of carotenoids-rich THF solution by changing pressure from 140bar to 160bar and time from 40min to 60min at feed flow rate of 0.2ml/min, CO2 flow rate of 15L/min and temperature of 318K was examined to find effects on the purity and recovery of trans-b-carotene. There experimental indeed produced micronized precipitates with mean particle size range from 3.5 micro meter to 19 micro meter and the purity of trans-b- carotene increased to 926.8mg/g with a recovery of 54%.
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