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標題: Functional Characterization of Cucumis metuliferus Proteinase Inhibitor Gene (CmSPI) in Potyviruses Resistance
作者: Lin, Chia-Wei
Su, Mei-Hsiu
Lin, Yu-Tsung
Chung, Chien-Hung
Ku, Hsin-Mei
關鍵字: Cucumis metuliferus;Papaya ringspot virus (PRSV);Potato virus Y (PVY);Proteinase inhibitors (PIs);RNA interference (RNAi);Base Sequence;Cucumis;Disease Resistance;Enzyme Inhibitors;Gene Expression Regulation, Plant;Molecular Sequence Data;Phylogeny;Plant Diseases;Plant Proteins;Potyvirus;Tobacco
Project: Viruses, Volume 7, Issue 7, Page(s) 3816-34.
Proteinase inhibitors are ubiquitous proteins that block the active center or interact allosterically with proteinases and are involved in plant physiological processes and defense responses to biotic and abiotic stresses. The CmSPI gene identified from Cucumis metuliferus encodes a serine type PI (8 kDa) that belongs to potato I type family. To evaluate the effect of silencing CmSPI gene on Papaya ringspot virus resistance, RNA interference (RNAi) with an inter-space hairpin RNA (ihpRNA) construct was introduced into a PRSV-resistant C. metuliferus line. CmSPI was down-regulated in CmSPI RNAi transgenic lines in which synchronously PRSV symptoms were evident at 21 day post inoculation. Alternatively, heterogeneous expression of CmSPI in Nicotiana benthamiana was also conducted and showed that CmSPI can provide resistance to Potato virus Y, another member of Potyvirus, in transgenic N. benthamiana lines. This study demonstrated that CmSPI plays an important role in resistant function against potyviruses in C. metuliferus and N. benthamiana.
DOI: 10.3390/v7072799
Appears in Collections:農藝學系

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