Chryseobacterium echinoideorum sp. nov., isolated from sea urchins (Tripneustes gratilla) in Penghu Island (Taiwan)

Abstract

A Gram-stain-negative, aerobic, non-motile rods, flexirubin-producing bacterium, designated strain CC-CZW010T, was isolated from the edible sea urchin Tripneustes gratilla in Penghu Island. The isolate grew optimally at pH 7.0 and 30ºС in the presence of 2% (w/v) NaCl. The most closely related strains in terms of 16S rRNA gene sequence similarity were Chryseobacterium taihuense NBRC 108747T (97.6%) and Chryseobacterium aquaticum KCTC 12483T (96.7%). Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CZW010T with respect to other Chryseobacterium species. Strain CC-CZW010T was determined to possess C15:0 iso, C15:0 anteiso, C17:0 iso 3OH, summed feature 3, comprising C16:1 ω7c/C16:1 ω6c and summed featured 9, comprising C16:0 10-methyl/C17:1 iso w9c as predominant fatty acids. The major polar lipid profile consisted of phosphatidylethanolamine, two unidentified lipids and five aminolipids. The polyamine pattern contains the major compound sym-homospermidine. Menaquinone (MK-6) was the predominant respiratory quinine; the G+C content of the genomic DNA was 36.4 mol%;. According to distinct phylogenetic, phenotypic and chemotaxonomic features, strain CC-CZW010T was proposed to represent a novel species within the genus Chryseobacterium, for which the name Chryseobacterium echinoideorum sp. nov. was proposed. The type strain is CC-CZW010T (=BCRC 80786T =JCM 30470T).