Thalassocola ureilytica gen. nov. sp. nov., of the family Phyllobacteriaceae isolated from seawater of South China Sea

Abstract

A Gram-staining-negative, aerobic, rod-shaped, non-flagellated marine bacterium, designated strain LS-861T, was isolated from seawater of South China Sea (Taiwan). Strain LS-861T grew optimally at pH 7.0 and 30ºС in the presence of 3% (w/v) NaCl. The novel strain shared highest 16S rRNA gene sequence similarity (91.5% each) to Nitratireductor shengliensis and Hoeflea halophila and lower sequence similarity (<91.5%) to other species. Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain LS-861T within the clade that accommodated members of the family Phyllobacteriaceae. The major fatty acids were C16:0, C17:1 iso ω10c, C18:0 3OH and C18:1 ω7c / C18:1 ω6c. The polar lipid profile was relatively simple as compared to other representatives of Phyllobacteriaceae, by having major amounts of diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid, and moderate amounts of three unidentified phospholipids and an unidentified aminolipid. The DNA G+C content was 61.2 mol%. The predominant quinone system was ubiquinone-10 (Q10). The polyphasic data in general and phylogenetic and polar lipid data in particular clearly distinguished the novel strain from the related species at genus level. Thus, strain LS-861T is proposed to represent a novel genus and species of the family Phyllobacteriaceae, for which the name Thalassocola ureilytica gen. nov., sp. nov. is proposed. The type strain of the type species LS-861T (=BCRC 80818T =JCM 30682T).