Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/94029
標題: Honokiol confers immunogenicity by dictating calreticulin exposure, activating ER stress and inhibiting epithelial-to-mesenchymal transition
作者: Liu, Shing-Hwa
Lee, Wen-Jane
Lai, De-Wei
Wu, Sheng-Mao
Liu, Chia-Yu
Tien, Hsing-Ru
Chiu, Chien-Shan
Peng, Yen-Chun
Jan, Yee-Jee
Chao, Te-Hsin
Pan, Hung-Chuan
Sheu, Meei-Ling
關鍵字: Calreticulin;Carcinogenesis;Endoplasmic reticulum stress;Epithelial-to-mesenchymal transition;Immunogenic cell death;Adult;Animals;Biomarkers, Tumor;Biphenyl Compounds;Calpain;Calreticulin;Cell Death;Cell Line, Tumor;Down-Regulation;Endoplasmic Reticulum Stress;Epithelial-Mesenchymal Transition;Female;Gene Knockdown Techniques;Humans;Lignans;Macrophages;Male;Methylnitronitrosoguanidine;Mice, Inbred BALB C;Mice, Inbred C57BL;Middle Aged;Neoplasm Invasiveness;PPAR gamma;Phagocytosis;Promoter Regions, Genetic;Protein Binding;Stomach Neoplasms;Up-Regulation
Project: Molecular oncology, Volume 9, Issue 4, Page(s) 834-49.
摘要: 
Peritoneal dissemination is a major clinical obstacle in gastrointestinal cancer therapy, and it accounts for the majority of cancer-related mortality. Calreticulin (CRT) is over-expressed in gastric tumors and has been linked to poor prognosis. In this study, immunohistochemistry studies revealed that the up-regulation of CRT was associated with lymph node and distant metastasis in patients with gastric cancer specimens. CRT was significantly down-regulated in highly metastatic gastric cancer cell lines and metastatic animal by Honokiol-treated. Small RNA interference blocking CRT by siRNA-CRT was translocated to the cells in the early immunogenic response to Honokiol. Honokiol activated endoplasmic reticulum (ER) stress and down-regulated peroxisome proliferator-activated receptor-γ (PPARγ) activity resulting in PPARγ and CRT degradation through calpain-II activity, which could be reversed by siRNA-calpain-II. The Calpain-II/PPARγ/CRT axis and interaction evoked by Honokiol could be blocked by gene silencing or pharmacological agents. Both transforming growth factor (TGF)-β1 and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) induced cell migration, invasion and reciprocal down-regulation of epithelial marker E-cadherin, which could be abrogated by siRNA-CRT. Moreover, Honokiol significantly suppressed MNNG-induced gastrointestinal tumor growth and over-expression of CRT in mice. Knockdown CRT in gastric cancer cells was found to effectively reduce growth ability and metastasis in vivo. The present study provides insight into the specific biological behavior of CRT in epithelial-to-mesenchymal transition (EMT) and metastasis. Taken together, our results suggest that the therapeutic inhibition of CRT by Honokiol suppresses both gastric tumor growth and peritoneal dissemination by dictating early translocation of CRT in immunogenic cell death, activating ER stress, and blocking EMT.
URI: http://hdl.handle.net/11455/94029
DOI: 10.1016/j.molonc.2014.12.009
Appears in Collections:生物醫學研究所

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