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標題: The glutathione peroxidase-mediated reactive oxygen species resistance, fungicide sensitivity and cell wall construction in the citrus fungal pathogen Alternaria alternata
作者: Yang, Siwy Ling
Yu, Pei-Ling
Chung, Kuang-Ren
關鍵字: Alternaria;Cell Wall;Chitin;Citrus;Dioxoles;Fungal Proteins;Fungicides, Industrial;Glutathione Peroxidase;Microbial Sensitivity Tests;Molecular Sequence Data;NADPH Oxidase;Oxazoles;Oxidative Stress;Pyrroles;Reactive Oxygen Species;Virulence
Project: Environmental microbiology, Volume 18, Issue 3, Page(s) 923-35.
The ability to detoxify reactive oxygen species (ROS) is critical for pathogenicity in the necrotrophic fungus Alternaria alternata. We report a glutathione peroxidase 3 (AaGPx3) involved in the complex signalling network that is essential for the detoxification of cellular stresses induced by ROS and for A. alternata pathogenesis in citrus. AaGPx3 deletion mutants displayed increased sensitivity to H2 O2 and many ROS-generating compounds. AaGPx3 is required for correct fungal development as the AaGPx3 mutant strains showed a severe reduction in conidiation. AaGPx3 mutants accumulated higher chitin content than the wild-type and were less sensitive to the cell wall-targeting compounds calcofluor white and Congo red, as well as the fungicides fludioxonil and vinclozolin, suggesting a role of the glutathione systems in fungal cell wall construction. Virulence assays revealed that AaGPx3 is required for full virulence. The expression of AaGPx3 was downregulated in fungal strains carrying defective NADPH oxidase (Nox) or the oxidative stress responsive regulators YAP1 and HOG1, all implicated in ROS resistance. These results further support the important role of ROS detoxification during A. alternata pathogenesis in citrus. Overall, our study provides genetic evidence to define the central role of AaGPx3 in the biological and pathological functions of A. alternata.
DOI: 10.1111/1462-2920.13125
Appears in Collections:植物病理學系

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