Please use this identifier to cite or link to this item:
|標題:||Ammoniphilus resinae sp. nov., an endospore-forming bacterium isolated from resin fragments||作者:||Lin, Shih-Yao
|關鍵字:||Bacillales;Bacterial Typing Techniques;Base Composition;DNA, Bacterial;Diaminopimelic Acid;Fatty Acids;Indonesia;Nucleic Acid Hybridization;Peptidoglycan;Phospholipids;RNA, Ribosomal, 16S;Sequence Analysis, DNA;Spermidine;Spores, Bacterial;Vitamin K 2;Phylogeny;Resins, Plant||Project:||International journal of systematic and evolutionary microbiology, Volume 66, Issue 8, Page(s) 3010-6.||摘要:||
A polyphasic approach was used to characterize an endospore-forming bacterium, designated strain CC-RT-ET, isolated from resin fragments. Strain CC-RT-ET was Gram-stain-variable and facultatively anaerobic, able to grow at 20-37 °C (optimal 30 °C) and pH6.0-9.0 (optimal pH 7.0). Ellipsoidal and terminal endospores were observed. The 16S rRNA gene sequence analysis of strain CC-RT-ET showed highest sequence similarity to Ammoniphilus oxalivorans RAOx-FST (97.8 %) and Ammoniphilus oxalaticus RAOx-1T (97.7 %). According to the DNA-DNA hybridization, the relatedness values of strain CC-RT-ET with Ammoniphilus oxalivorans RAOx-FST and Ammoniphilus oxalaticus RAOx-1T were 21 % and 29 %, respectively. The DNA G+C content was 39.2 mol% and the predominant quinone system was menaquinone-7 (MK-7). The major polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylmethylethanolamine. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The polyamine pattern showed predominance of sym-homospermidine and putrescine. The major fatty acids found in strain CC-RT-ET were C14 : 0, iso-C15 : 0, anteiso-C15 : 0 and C16 : 1ω7c/C16 : 1ω6c. Based on the distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence analysis, strain CC-RT-ET is considered to represent a novel species of the genus Ammoniphilus, for which the name Ammoniphilus resinaesp. nov. is proposed. The type strain is CC-RT-ET (=BCRC 80314T=DSM 24738T).
|Appears in Collections:||土壤環境科學系|
Show full item record
Files in This Item:
TAIR Related Article
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.