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標題: Azospirillum agricola sp. nov., a nitrogen-fixing species isolated from cultivated soil
作者: Lin, Shih-Yao
Liu, You-Cheng
Hameed, Asif
Hsu, Yi-Han
Huang, Hsin-I
Lai, Wei-An
Young, Chiu-Chung
關鍵字: Azospirillum;Bacterial Typing Techniques;Base Composition;DNA, Bacterial;Fatty Acids;Nitrogen;Nucleic Acid Hybridization;Phospholipids;RNA, Ribosomal, 16S;Sequence Analysis, DNA;Taiwan;Ubiquinone;Nitrogen Fixation;Phylogeny;Soil Microbiology
Project: International journal of systematic and evolutionary microbiology, Volume 66, Issue 3, Page(s) 1453-8.
A polyphasic approach was used to characterize a novel nitrogen-fixing bacterial strain, designated CC-HIH038T, isolated from cultivated soil in Taiwan. Cells of strain CC-HIH038T were Gram-stain-negative, facultatively aerobic and spiral-shaped, with motility provided by a single polar flagellum. The 16S rRNA gene sequence analysis of strain CC-HIH038T showed highest sequence similarity to Azospirillum doebereinerae (98.0 %), Azospirillum thiophilum (97.5 %), Azospirillum rugosum (97.4 %) and Azospirillum zeae (97.2 %) and lower sequence similarity ( < 97.0 %) to all other species of the genus Azospirillum. According to DNA-DNA association, the relatedness values of strain CC-HIH038T with A. doebereinerae, A. thiophilum, A. rugosum and A. zeae were 51.8 %, 41.2 %, 56.5 % and 37.5 %, respectively. Strain CC-HIH038T was able to grow at 20-37 °C and pH 7.0-8.0. Strain CC-HIH038T gave positive amplification for dinitrogen reductase (nifH gene); the activity was recorded as 8.4 nmol ethylene h- 1. The predominant quinone system was ubiquinone Q-10 and the DNA G+C content was 68.8 mol%. The major fatty acids found in strain CC-HIH038T were C16 : 0, iso-C18 : 0, C16 : 0 3-OH, C14 : 0 3-OH/iso-C16 : 1 and C18 : 1ω7c/C18 : 1ω6c. Based on the distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence analysis, strain CC-HIH038T is considered to represent a novel species in the genus Azospirillum, for which the name Azospirillum agricola sp. nov. is proposed. The type strain is CC-HIH038T ( = BCRC 80909T = JCM 30827T).
DOI: 10.1099/ijsem.0.000904
Appears in Collections:土壤環境科學系

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