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標題: | Direct Electrochemical Sensing of Phenazine-1-carboxylic Acid Secreted by Pseudomonas chlororaphis subsp. aureofaciens BCRC 11057T Using Disposable Screen-printed Carbon Electrode | 作者: | Asif Hameed Hong-Wei Pi Shih-Yao Lin Wei-An Lai Li-Sen Young You-Cheng Liu Fo-Ting Shen Chiu-Chung Young 哈錫夫 |
關鍵字: | Phenazine;Cyclic voltammetry;Electropolymerization;Preanodization;Pseudomonas aeruginosa | Project: | Electroanalysis, Volume 28, Issue 4, pages 846–853, April 2016 | 摘要: | A novel electrochemical approach for direct recognition of antibiotic phenazine-1-carboxylic acid (PCA) was developed. PCA was electropolymerized on preanodized screen-printed carbon electrode (SPCE*-PCA) through repetitive cyclic voltammetry and characterized by XPS and FESEM. Electron transfer involved intermediate phenomenon of diffusion-controlled redox process and surface bound redox reaction. At pH 8 (optimum), SPCE*-PCA had a detection limit of 0.51±0.04 μM, a quantification limit of 1.7±0.13 μM, linearity of up to 50 µM, a repeatability of 15.5 % and a reproducibility of 1.7 %. PCA secreted by Pseudomonas chlororaphis subsp. aureofaciens BCRC 11057T was investigated successfully using present single run approach. |
URI: | http://hdl.handle.net/11455/94168 | DOI: | 10.1002/elan.201500278 |
Appears in Collections: | 土壤環境科學系 |
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