Direct Electrochemical Sensing of Phenazine-1-carboxylic Acid Secreted by Pseudomonas chlororaphis subsp. aureofaciens BCRC 11057T Using Disposable Screen-printed Carbon Electrode

Abstract

A novel electrochemical approach for direct recognition of antibiotic phenazine-1-carboxylic acid (PCA) was developed. PCA was electropolymerized on preanodized screen-printed carbon electrode (SPCE*-PCA) through repetitive cyclic voltammetry and characterized by XPS and FESEM. Electron transfer involved intermediate phenomenon of diffusion-controlled redox process and surface bound redox reaction. At pH 8 (optimum), SPCE*-PCA had a detection limit of 0.51±0.04 μM, a quantification limit of 1.7±0.13 μM, linearity of up to 50 µM, a repeatability of 15.5 % and a reproducibility of 1.7 %. PCA secreted by Pseudomonas chlororaphis subsp. aureofaciens BCRC 11057T was investigated successfully using present single run approach.