Please use this identifier to cite or link to this item:
|標題:||Thin-Film Transistor-Based Biosensors for Determining Stoichiometry of Biochemical Reactions||作者:||Wang, Yi-Wen
|關鍵字:||Biosensing Techniques;Electric Conductivity;Kinetics;NAD;Oxaloacetic Acid;Transistors, Electronic||Project:||PloS one, Volume 11, Issue 12, Page(s) e0169094.||摘要:||
The enzyme kinetic in a biochemical reaction is critical to scientific research and drug discovery but can hardly be determined experimentally from enzyme assays. In this work, a charge-current transducer (a transistor) is proposed to evaluate the status of biochemical reaction by monitoring the electrical charge changes. Using the malate-aspartate shuttle as an example, a thin-film transistor (TFT)-based biosensor with an extended gold pad is demonstrated to detect the biochemical reaction between NADH and NAD+. The drain current change indicates the status of chemical equilibrium and stoichiometry.
|Appears in Collections:||生醫工程研究所|
Show full item record
Files in This Item:
TAIR Related Article
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.