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標題: A Common Flanking Region in Promiscuous Plasmids Encoding blaNDM-1 in Klebsiella pneumoniae Isolated in Singapore
作者: Chen, Ying-Tsong
Siu, L Kristopher
Tsai, Yu-Kuo
Lin, Fu-Mei
Koh, Tse Hsien
Chen, Jiun-Han
關鍵字: Anti-Bacterial Agents;Aztreonam;Base Sequence;Ciprofloxacin;Conjugation, Genetic;Conserved Sequence;DNA, Bacterial;Drug Resistance, Multiple, Bacterial;Electrophoresis, Gel, Pulsed-Field;Gene Expression;Gentamicins;Humans;Klebsiella Infections;Klebsiella pneumoniae;Microbial Sensitivity Tests;Multilocus Sequence Typing;Plasmids;Singapore;Tetracycline;Trimethoprim, Sulfamethoxazole Drug Combination;beta-Lactamases
Project: Microbial drug resistance (Larchmont, N.Y.), Volume 22, Issue 2, Page(s) 109-14.
Bacteria encoding the New Delhi metallo-β-lactamase gene (blaNDM-1) are regarded as superbugs for their resistance to multiple antibiotics. Plasmids encoding blaNDM-1 have been observed to be spreading among gram-negative bacteria around the world. Previous studies have demonstrated that multiple modifications of blaNDM-1-harboring plasmids might contribute to the spread of the gene. In this study, we analyzed blaNDM-1-encoding plasmids from two Klebsiella pneumoniae isolates, DU7433 and DU1301, found to be unrelated by pulsed field gel electrophoresis and multilocus sequencing typing (DU7433: ST14 and DU1301: ST11), and compared them with previously published plasmids. Although strains DU1301, DU7433, and previously published strain DU43320 carried unrelated plasmids, their transconjugants exhibited similar antimicrobial resistance profiles. Transconjugants lacked the resistance to aztreonam, ciprofloxacin, gentamicin, tetracycline, and trimethoprim/sulfamethoxazole when compared with the corresponding clinical isolates. Plasmids pTR1 from DU1301 and pTR2 from DU7433 had completely different plasmid backbones except a short conserved region of blaNDM-1 and ble flanked with truncated or nontruncated ISAba125 and trpF. The presence of this common region among known blaNDM-1-carrying plasmids implies that the dissemination of blaNDM-1 may be facilitated by mobilization of this conserved immediate region among different plasmids. Control measures should be strictly enforced whenever increasing incidences of epidemiological unrelated strains were identified.
DOI: 10.1089/mdr.2015.0132
Appears in Collections:基因體暨生物資訊學研究所

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