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|標題:||T-cell factor-4 and MHC upregulation in pigs receiving a live attenuated classical swine fever virus (CSFV) vaccine strain with interferon-gamma adjuvant||作者:||Fan, Y-H
|關鍵字:||Classical swine fever virus;IgG2 isotype;Interferon-gamma;Major histocompatibility complex;Porcine;T-cell factor-4;Animals;Biomarkers;Classical Swine Fever;Classical swine fever virus;Genes, MHC Class I;Genes, MHC Class II;Immunologic Factors;Interferon-gamma;Swine;Transcription Factor 7-Like 2 Protein;Vaccines, Attenuated;Viral Vaccines||Project:||Veterinary journal (London, England : 1997), Volume 216, Page(s) 148-56.||摘要:||
The effect of co-administration of interferon (IFN)-γ in pigs undergoing vaccination with an attenuated strain (LPC) of classical swine fever virus (CSFV) was investigated. Unvaccinated pigs demonstrated pyrexia and died 7-9 days after challenge with virulent CSFV. Pigs receiving the attenuated vaccine remained healthy after virus challenge, except for mild, transient pyrexia, whereas pigs receiving IFN-γ simultaneously with the vaccine demonstrated normal body temperatures after virus challenge. Examination by nested RT-PCR revealed greater viral load in the spleens of the pigs vaccinated with the attenuated CSFV, compared with those that had additionally received IFN-γ. Expression of major histocompatibility complex (MHC) class I and MHC class II molecules was upregulated in the spleens of the IFN-γ treated vaccinated pigs, demonstrated by immunohistochemistry. Based on Western blot analysis, anti-CSFV IgG2 antibodies were elevated in vaccinated pigs by co-administration of IFN-γ (IFN-γ(Hi): P < 0.01; IFN-γ(Lo): P <0.05). By employing the suppression subtractive hybridization technique, RT-PCR, in situ hybridization, and immunohistochemistry, T-cell factor-4 (Tcf-4) mRNA and protein expression were found to be upregulated in the spleens of vaccinated pigs that had received IFN-γ. This study suggests involvement of Tcf-4 in IFN-γ-mediated immune regulation following CSFV vaccination.
|Appears in Collections:||微生物暨公共衛生學研究所|
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