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標題: Biocompatibility assessment of nanomaterials for environmental safety screening
作者: Lee, Yi-Hui
Chuang, Show-Mei
Huang, Sheng-Chi
Tan, Xiaotong
Liang, Ruei-Yue
Yang, Gordon C C
Chueh, Pin Ju
關鍵字: cell-impedance measurement;cytotoxicity;in vitro assays;nanomaterials;A549 Cells;Animals;Apoptosis;Cell Proliferation;Cell Survival;Humans;Materials Testing;Metal Nanoparticles;Mice;NIH 3T3 Cells;Oxidative Stress;Particle Size
Project: Environmental toxicology, Volume 32, Issue 4, Page(s) 1170-1182.
In view of the extensive use of nanoparticles in countless applications, a fast and effective method for assessing their potential adverse effects on the environment and human health is extremely important. At present, in vitro cell-based assays are the standard approach for screening chemicals for cytotoxicity because of their relative simplicity, sensitivity, and cost-effectiveness compared with animal studies. Regrettably, such cell-based viability assays encounter limitations when applied to determining the biological toxicity of nanomaterials, which often interact with assay components and produce unreliable outcomes. We have established a cell-impedance-based, label-free, real-time cell-monitoring platform suitable for use in a variety of mammalian cell lines that displays results as cell index values. In addition to this real-time screening platform, other traditional cytotoxicity assays were employed to validate cytotoxicity assessments. We suggest that the cell impedance measurement approach is effective and better suited to determining the cytotoxicity of nanomaterials for environmental safety screening. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 1170-1182, 2017.
DOI: 10.1002/tox.22313
Appears in Collections:生醫工程研究所

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