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標題: Ochratoxin A induces ER stress and apoptosis in mesangial cells via a NADPH oxidase-derived reactive oxygen species-mediated calpain activation pathway
作者: 許美鈴
Sheu, Meei-Ling
Shen, Chin-Chang
Chen, Yuan-Siao
Chiang, Chih-Kang
關鍵字: ER stress;NADPH oxidase;apoptosis;mesangial cells;ochratoxin A;Animals;Apoptosis;Calcium Channel Blockers;Calpain;Cell Proliferation;Cells, Cultured;Endoplasmic Reticulum Stress;Mesangial Cells;Mice;NADPH Oxidases;Ochratoxins;Oxidation-Reduction;Oxidative Stress;Rats;Reactive Oxygen Species;Signal Transduction
Project: Oncotarget, Volume 8, Issue 12, Page(s) 19376-19388.
Ochratoxin A (OTA) contaminated food increases reactive oxygen species (ROS) production in glomerulus and causes glomerulopathy. The molecular mechanisms still remain uncertain. In this study, we used mouse and rat glomerular mesangial cells and delineate the signaling pathway behind the OTA-triggered cell apoptosis. OTA dose-dependently induced expression of ER stress markers including phospho-PERK, phospho-eIF2α, GRP78, GRP94, and CHOP. Apoptosis events including cleavage of caspase-12, caspase-7, and PARP are also observed. OTA activated oxidative stress and increased NADPH oxidase activity. NADPH oxidase inhibitor, apocynin, significantly attenuated OTA-induced cell apoptosis. Moreover, OTA markedly increased the calpain activity which significantly inhibited by apocynin. Transfection of calpain-siRNA effectively inhibited the OTA-increased ER stress-related protein expression. These findings suggest that OTA activated NADPH oxidase and calpain, induced ER stress and ROS production, and caused glomerular mesangial cells apoptosis which leads to glomerulopathy.
DOI: 10.18632/oncotarget.14270
Appears in Collections:生物醫學研究所

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