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標題: Develop an immunomagnetic bead based lateral flow assay for the rapid detection of a major peanut allergen – Ara h 2
建立一種免疫磁珠側流層析檢測方法以快速偵測花生主要過敏原Ara h 2
作者: Yang-Fan Fung
關鍵字: 花生過敏原;Ara h 2;磁珠;測流免疫層析;peanut allergen;Ara h 2;magnetic beads;lateral flow assay
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花生被廣泛的用於各種食品和食品加工,包括各類點心、餅乾、飲料、糖果等。然而花生含有會引發嚴重過敏反應的過敏原,嚴重者會造成過敏性休克。因此,建立一個快速檢測花生過敏原的檢測法是非常必要的。Ara h 2是花生一個主要的過敏原,相比比起Ara h,它十分穩定並且在食品加工過程中不容易被降解,所以本研究針對Ara h 2建立了一個磁珠側流免疫層析快篩檢測法。首先,本研究對兩株勝肽抗體和一株Anti-Ara h 2多住抗體進行配對,一個抗體用於修飾磁珠,另一個則作為檢量線塗佈的抗體;然後,對磁珠和免疫試紙片進行條件優化,確認條件為: NC膜型號AE99、檢量線抗體密度7.5 μg/cm、磁珠大小100 nm、修飾磁珠的抗體濃度400 μg/ml、NC膜Blocker為glycine、磁珠Blocker為Milk、磁珠樣品反應比例20:300、反應時間30分鐘;此外,本研究還對此法的存儲性和精準度進行了測試。本研究成功建立了該磁珠側流免疫層析檢測法的模型,它的檢測極限為0.5 μg/ml,標準曲線方程y = -0.1207x2 + 7.7389x + 2.0215,R² = 0.9997。最後,本法用於添加十種市售油品的檢測。

Peanut has been widely used in various kinds of food products, including snacks, biscuits, drinks and candies. However, peanut also induces severe allergic reactions, such as anaphylaxis shock. Thus, it is necessary to develop a rapid method for detecting peanut residues. Ara h2 is a major peanut allergen and its stability gets less impacted by food processing than another major peanut allergen- Ara h1. Therefore, in this study, we developed a magnetic bead labeled lateral flow assay to detect a major peanut allergen- Ara h2. Firstly, two anti-peptide antibodies and one anti-Ara h2 polyclonal antibody were used to determine an optimal pair of antibody: one antibody tagged on the surface of magnetic bead and the other immobilized on the test line of a lateral flow strip. Then, magnetic beads and NC membranes were optimizated. Following is the final condition, NC membrane type is AE99, testline antibody density is 7.5 μg/cmm magnetic beads size is 100 nm, modified antibody concentration is 400 μg/ml, NC membrane blocker is glycine, magnetic beads blocker is milk, ratio of magnetic beads and sample is 20:300, reaction time is 30 min. Furthermore, storability and inter/intra assay were tested. Therefore, a rapid magnetic bead labeled lateral flow assay was developed. LOD is 0.5 μg/ml. y = -0.1207x2 + 7.7389x + 2.0215, R² = 0.9997. Finally, this developed assay was used to detect Ara h 2 in oil product samples.
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Appears in Collections:食品暨應用生物科技學系

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