Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/96059
標題: Effect of camellia oil on improvement of ethanol-induced gastrointestinal mucosal injury and regulation of gut microbiota
苦茶油改善乙醇誘導腸胃黏膜損傷及調節腸道菌相之研究
作者: Wei-Ting Li
李瑋婷
關鍵字: 苦茶油;胃潰瘍;乙醇;腸道菌相;胃腸保健;Camellia oil;gastric ulcer;ethanol;gut microbiota;gastrointestinal health
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摘要: 
由於現代人飲食型態改變、生活壓力大以及酗酒等不良習慣,胃腸道功能紊亂成為現今常見的通病,如:消化性潰瘍、腸躁症等。苦茶油為台灣常見食用油品,於民間療法中被認為具有腸胃保健之功效。本研究室先前的研究指出,苦茶油具有預防酒精性潰瘍之功效,為進一步證實不同來源或製程之苦茶油是否有相同功效,以及改善腸道菌相組成之潛力。故本研究利用體外 (in vitro) 及體內 (in vivo) 試驗模式進行探討。在乙醇誘導胃黏膜損傷模式中,利用大鼠胃黏膜細胞 (RGM-1) 及 BALB/c 小鼠進行試驗。在評估腸道菌相模式中,則是透過 SD 大鼠進行試驗。結果顯示,RGM-1 細胞以不同苦茶油預處理後皆可減緩乙醇誘導之細胞毒性,最後選擇 BLCO 苦茶油進行動物試驗。。動物實驗結果顯示,乙醇 (5 mL/kg b.w.) 誘導一小時後會造成胃黏膜損傷。然而,餵食苦茶油 (1 及 2 mL/kg b.w.) 三週或 Lansoprazole (30 mg/kg b.w.) 一週後能有效改善乙醇誘導小鼠之胃損傷,提升黏膜黏液之分泌和抗氧化酵素活性,包括:觸酶 (Catalase)、麩胱甘肽-S-轉移酶 (GST)、麩胱甘肽過氧化酶 (GPx) 及麩胱甘肽還原酶 (GRd);與減緩酒精誘導胃組織發炎因子 COX-2 及 IL-1β 之蛋白表現。於腸道菌相部分,大鼠餵食苦茶油、橄欖油與大豆油三週後,糞便菌相組成以 16S rRNA 進行定序分析。結果顯示,餵食大鼠苦茶油與大豆油後,其糞便微生物群聚組成差異較大。此外,相較於橄欖油與大豆油組,攝食苦茶油有提升腸道微生物之 Firmicutes/Bacteroidetes 比率、α 多樣性、共生菌與益生菌 Bifidobacterium 豐富度,以及降低 Prevotella 之趨勢。綜合上述,苦茶油能透過降低乙醇所誘導的氧化傷害及發炎作用,以達到減緩乙醇所誘導的胃損傷,並且改善腸道微生物之組成與增加其多樣性。因此,本研究成果可作為未來開發苦茶油為胃腸相關機能性食品之參考,以提升本土農產品附加價值。

The gastrointestinal disorders such as peptic ulcer and irritable bowel syndrome become a common problem nowadays, which is due to the dietary habit changes, stressed lives and alcoholism. Camellia oil is commonly used in Taiwan and has been shown with healthy effects for gastrointestinal tract in folk medicine. Our previous study has been demonstrated that camellia oil could prevent the gastric mucosal damage caused by ethanol. This study was aimed to further confirm whether the effects of different sources or processes of camellia oils have the same effect. In addition, the influence of camellia oil on regulation of gut microbiota was also investigated by SD rats. In this study, the RGM-1 cells and BALB/c mice were used in ethanol-induced gastric mucosal damage model. The results indicated that RGM-1 cells pretreated with camellia oils could reduce the decrease of ethanol-induced cell viability and finally the BLCO camellia oil was chosen for further animal experiment. Animal experiments showed that ethanol (5 mL/kg b.w.) for an hour can cause gastric mucosal injury. However, pretreatment of mice with camellia oil (1 and 2 mL/kg b.w.) for 3 weeks and lansoprazole (30 mg/kg b.w.) for 1 week effectively improved ethanol-induced gastric injury, enhanced mucosal mucus secretion, antioxidant enzyme activities including catalase, GST, GPx and GRd, and alleviated inflammatory cytokines by suppressing the production of COX-2 and IL-1β induced by ethanol in gastric tissue. In the study of gut microbiota, rats were fed with camellia oil, olive oil or soybean oil for 3 weeks, and the fecal microbiota were analyzed using 16S rRNA gene sequencing. The results of gut microbiota showed significant clustering of feces in SD rats between pretreatment with camellia oil and soybean oil; however, the individual differences of olive oil group varied considerably. When compared with the soybean oil and olive oil groups, the intake of camellia oil could increase Firmicutes to Bacteroidetes ratio, the alpha diversity, commensal bacteria and Bifidobacterium abundance, and reduced the Prevotella of gut microbiota. In conclusion, the camellia oil could alleviate the ethanol-induced gastric injury by reducing oxidative damage and inflammation. Moreover, camellia oil in the diet could improve the compositions of gut microbiota and increase its diversity. Therefore, the results of this study can be applied to develop the gastrointestinal-related functional food in the future, and thereby enhance the adding values of indigenous agricultural products.
URI: http://hdl.handle.net/11455/96059
Rights: 同意授權瀏覽/列印電子全文服務,2020-08-28起公開。
Appears in Collections:食品暨應用生物科技學系

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