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標題: 分析鹿谷鄉農會比賽茶中各兒茶素的組成
Analysis of catechin contents of Oolong tea contestants from Lugu Farmers' Association Tea Competition
作者: 柯元敦
Yuan - Dun Ke
關鍵字: 凍頂烏龍茶;比賽茶;兒茶素;Dong Ding Oolong Tea;tea competition;catechin
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茶在世界上是很重要的經濟作物,而烏龍茶在臺灣傳統上則是最具知名度的飲品。先前研究中,有很多證據顯示茶葉對身體健康有正面的影響,而兒茶素是主要的有效成分之一,同時也是影響茶葉風味的因素。鹿谷鄉農會比賽茶競賽是在 1976 年創立的,是最具代表性的競賽之一,因為分級制度明確,品質與風味優良,因此深具公信力。本研究之目的為利用鹿谷鄉農會比賽茶中不同獲獎等級之茶樣為材料,以分析其中兒茶素的組成與含量,並可作為凍頂烏龍茶的兒茶素含量之參考依據。實驗的茶樣品主要選自 2016 年鹿谷鄉農會比賽茶競賽的春茶與冬茶,利用不同的方式萃取茶湯,包括前人研究的萃取方式及依照比賽茶沖泡規則,再以高效能液相層析儀分析凍頂烏龍茶中的兒茶素組成與含量。實驗結果顯示,兩個萃取方式皆顯示表沒食子兒茶素與表沒食子兒茶素沒食子酸酯是凍頂烏龍茶主要的兒茶素類成分;依照比賽茶方式萃取茶湯,表沒食子兒茶素為含量最高的兒茶素。目前實驗分析結果,不同等級的鹿谷鄉農會比賽茶中各兒茶素含量沒有明顯的相關性,但此數據也能作為了解飲用凍頂烏龍茶的兒茶素攝入量之參考依據及茶品內容標示所用。另外還進行其他比賽茶競賽的茶樣品分析,包括文山包種茶、阿里山高山茶、東方美人茶與日月潭紅茶,藉以了解與比較臺灣茶產品中的兒茶素含量,結果顯示採用青心烏龍製作的輕發酵烏龍茶 (凍頂烏龍茶、文山包種茶、阿里山高山茶),其主要的兒茶素為表沒食子兒茶素與表沒食子兒茶素沒食子酸酯,日月潭紅茶主要的兒茶素為表兒茶素沒食子酸酯,東方美人茶總兒茶素含量則相對較少。

Tea is an important cash crop in the world. Traditionally, Oolong tea is the most famous drink in Taiwan. Previous studies have shown that tea may have advantageous health effects. Catechins are the major active components and the supposed flavor determinant factor. The Lugu Farmers' Association Tea Competition is established in 1976 and the most representative committee, which rank the tea contestants seriously for good quality and flavor to maintain their high credibility. The purpose of this study was to analyze the tea evaluation standards based on catechin compositions and ratios in contestant samples from Lugu Farmers' Association Tea Competition, in an attempt to create a catechin contents label for Dong Ding Oolong Tea. In this study, we selected tea samples with different rankings from the 2016 (spring and winter) Tea Competition for analysis. Catechins extractions were prepared according to previous studies or the standards of tea competition and subjected to analyses using HPLC for compositions and ratios. Results of both extraction methods showed that Epigallocatechin (EGC) and Epigallocatechin gallate (EGCG) are the main compounds of catechins in Dong Ding Oolong Tea. EGC is the richest component in tea infusion prepared following tea competition standards. Currently, there is no obvious correlation between catechin contents and the rankings in the Lugu Farmers' Association Tea Competition. Nevertheless, the current results can be used as a reference for catechins intake and also as the label of content for Dong Ding Oolong Tea. In addition, we selected tea samples from other tea competition (Wenshan Pouchong Tea、Ali Mountain Oolong、Sun Moon Lake Black Tea and Oriental Beauty Tea) for analysis and comparison. Results of both extraction methods showed that EGC and EGCG are the main compounds of catechins in the light fermented oolong tea (Dong Ding oolong tea, Wenshan Pouchong Tea and Ali Mountain Oolong). Epicatechin gallate (ECG) is the main compound of catechins in Sun Moon Lake Black Tea. In contrast with the other tea, Oriental Beauty Tea catechins content is relatively less in this study.
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