Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/96399
標題: 野生稻 Oryza nivara 的 Bph31(t)抗褐飛蝨 基因座之精細定位與抗性分析
Fine mapping and resistance characterization of Bph31(t), a brown planthopper resistance locus from wild rice Oryza nivara
作者: 宋怡萱
Yi-Hsuan Song
關鍵字: 褐飛蝨;BPH
引用: Du, B., Zhang, W., Liu, B., Hu, J., Wei, Z., Shi, Z., et al. 106 (2009) 'Identification and characterization of Bph14, a gene conferring resistance to brown planthopper in rice.' Proceedings of the National Academy of Sciences, pp. 22163-22168 52. Fraser, C. M., & Chapple, C. (2009) 'The phenylpropanoid pathway in arabidopsis'. The Arabidopsis Book. Hao, P., Liu, C., Wang, Y., Chen, R., Tang, M., Du, B., et al. 146 (2008) 'Herbivore-Induced Callose Deposition on the Sieve Plates of Rice: An Important Mechanism for Host Resistance'. Plant Physiology, pp. 1810-1820 4. Ji, H., Kim, S.-R., Kim, Y.-H., Shu, J.-P., Mi, H., Nese, S., et al. 6:34376 (2016) 'Map-based Cloning and Characterization of the BPH18 Gene from Wild Rice Conferring Resistance to Brown Planthopper (BPH) Insect Pest'. Scientific Reports. Ling, Y., & Zhang, W. 35 (2016) 'Genetic and biochemical mechanisms of rice resistance to planthopper'. Springer Berlin Heidelberg, pp. 1559-1572. Lorieux, M. 30 (2012) 'MapDisto: fast and efficient computation of genetic linkage maps'. Molecular Breeding, pp. 1231-1235. Navdeep, K., Sharma, I., Kirat, K., & Pati, P. K. 6 (2016) 'Detection of reactive oxygen species in oryza sativa L. (Rice)'. Bioprotocol. Preetinder, S., & Sanmallappa, J. 23 (2016) 'Antixenosis and tolerance of rice genotypes against brown planthopper'. Rice Science, pp. 96-103. R., W. R., Paul, M., & Vineetha, M. V. 3 (2013) 'Estimation of salicylic acid in eucalytpus leaves using spectrophotometric methods'. Genetics and Plant Physiology, pp. 90-97 1-2. Wang, H., Ye, S., & Mou, T. 9 (2016) 'Molecular Breeding of Rice Restorer Lines and Hybrids for Brown Planthopper (BPH) Resistance Using the Bph14 and Bph15 Genes'. springer open. 21 Wang, Y., Cao, L., Zhang, Y., Cao, C., Liu, F., Huang, F., et al. 66 (2015) 'Map-based cloning and characterization of BPH29, a B3 domain-containing recessive gene conferring brown planthopper resistance in rice'. Experimental Botany, pp. 6035–6045. War, A. R., Paulraj, M. G., Ahmad, T., Buhroo, A. A., Hussain, B., Ignacimuthu, S., et al. 7 (2012) 'Mechanisms of Plant Defense against Insect Herbivores'. Plant Signaling & Behavior, pp. 1306-1320 10. Yasumori, T., Hattori, M., Yoshioka, H., Yoshioka, M., Takahashui, A., Wu, J., et al. 4 (2014) 'Map-based Cloning and Characterization of a Brown Planthopper Resistance Gene BPH26 from Oryza sativa L. ssp. indica Cultivar ADR52 '. Scientific Reports, pp. 5872-5880. 林慶元 8 (2007) '植物保護圖鑑系列 ‐ 水稻保護'. 行政院農業委員會動植物防 疫檢疫局, pp. 75-83. 蕭榮福, 范國洋, 陳漢洋, 謝忠能, & 李麗娟 (1985) '台灣省水稻病蟲害發生預 測 (1966-1984)'. 台灣省政府農林廳編印 382. 謝尚潜, & 章元明 (2012) '偏分離群體遺傳圖矯正和 QTL 定位軟件包 DistortedMap 的研製 '. 中國科技論文在線. 鄭清煥 (1980) '可能影響水稻害蟲藥劑防治效果及收益之若干因素'. 農藥研究 專題討論會講稿集, pp. 89-118. 鄭清煥 (2002) '水稻害蟲綜合管理研究之回顧與展望'. 台灣作物病蟲害綜合管 理研討會專刊, pp. 9-38. 鄭清煥, & 黃守宏 (2007) '作物蟲害之非農藥防治技術'. 行政院農業委員會農業 試驗所, pp. 57-70. 陳隆澤, 黃守宏, & 鄭清煥 (2009) '水稻病蟲害抗性檢定工作回顧'. 台灣水稻保 護成果及新展望研討會專刊, pp. 83‐103.
摘要: 
褐飛蝨(Nilaparvata lugens Stål., BPH)在亞洲地區為具高傷害性的水稻單食 性昆蟲,褐飛蝨以刺吸式口器吸取水稻韌皮部汁液並可能間接傳播病毒,導致水 稻產量嚴重減少。台灣農業試驗所(TARI)將印度野生稻 O. nivara (IRGC 102165, W33)中的顯性褐飛蝨抗性基因 Bph31(t)導入品種「台農 71 號」(TNG71)中,最 後選育出品系 KN210 (TNG71-Bph31(t)),根據農試所的研究,Bph31(t)基因座位 於第 4 號染色體上,且對褐飛蝨的抗性變異解釋量(Proportions of Variance Explained, PVE)高達 35.2% (LOD:22.2),在褐飛蝨抗性秧苗期檢定法(Standard Seedbox Screening Test, SSST)和半成株期檢定法(Modified seedbox screening test, MSST)中,品系 KN210 對褐飛蝨的抗性反應為 3.6(中抗等級),而 TNG71 則高達 7(敏感等級)。在本實驗中,我們使用在 Bph31(t)基因座區間系為異質結合體基因 型的植株作為分離族群之親本,自交後取 937 棵分離後裔用於 Bph31(t)基因精細 定位分析(fine mapping),從 27 個自行設計的 Indel 或 SSR 多型性標誌,選擇其 中 3 個用於篩選重組品系,最後確認其中有 37 棵植株在分子標誌 Indel774-1 至 RM16685 的 1744 kb 區間內發生重組。鑑定這 37 棵 F2 植株與其 F2:3子代的褐飛 蝨抗性,期望能縮小 Bph31(t)在染色體上的可能區間。此外,利用 F2:3植株進行 兩部分實驗,一部分是褐飛蝨對抗感品系間的習性觀察,發現褐飛蝨對於抗感植 物具有選擇性,偏好選擇 KN188 感性植株,且會從 KN210 移動至 KN188,食用 抗性品系之褐飛蝨,生長速度較低且死亡率較高;另一是關於褐飛蝨刺吸式取食 後水稻產生的變化,在 KN188 上能觀察到比 KN210 有更多的取食的傷口、ROS 產量等,同時抗性植株會累積 callose,可能因而阻塞褐飛蝨吸食而造成抗性。
URI: http://hdl.handle.net/11455/96399
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